본문 바로가기
KERD
논문KCI 등재

두시하태독법의 Th2 분화 조절을 통한 알레르기 비염 완화 효과

Reduction of Allergic Rhinitis by Controlling the Th2 Differentiation of Douchi Hataedock

안상현|김기봉|최준용
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2017.09|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제38권 제4호|pp. 468-478

1

조회

0

다운로드

3

인용

0

참고문헌

키워드

allergic rhinitisinflammationDouchiTh2 skewed conditionHataedock
주제분류한의학

초록

Objectives: This study investigated the effects of Douchi Hataedock on Th2-skewed conditions to control allergic rhinitis. Methods: NC/Nga mice were divided into three groups: 10 mice were assigned to the control group (CTRL; no treatment), 10 mice to allergic rhinitis-induced (ARE) without treatment group, and 10 mice to the allergic rhinitis-induced (FGT) after Douchi Hataedock treatment group. The 3-week-old mice of the FGT group were given one 10 mg/kg dose of Douchi Hataedock extract and resensitized to allergic antigens at weeks four, five, and six. Allergic rhinitis was induced primarily in mice nasal cavities for five days after one week of final sensitization. The second induction used the same method one week after the first induction was completed. After one week, the nasal mucosal tissues of each group were observed. Immunohistochemical staining for IL-4, STAT6, CD40, FcεRI, substance P, MMP-9, NF-κB p65, p-IkB, and iNOS in the nasal mucosa was also performed. Results: The FGT group had less respiratory epithelial damage and less mucin secretion in goblet cells than the ARE group and showed a 62% decrease in IL-4, 85% decrease in STAT6, 71% decrease in CD40, 69% decrease in FcɛRI, 43% decrease in substance P, 49% decrease in MMP-9, 43% decrease in NF-kB p65, 38% decrease in p-IkB, and 73% decrease in iNOS compared to the ARE group. Conclusions: Douchi Hataedock lessens inflammation in epithelial and goblet cells and reduces inflammatory mediator secretion in a mouse allergic rhinitis model.

Abstract

Objectives: This study investigated the effects of Douchi Hataedock on Th2-skewed conditions to control allergic rhinitis. Methods: NC/Nga mice were divided into three groups: 10 mice were assigned to the control group (CTRL; no treatment), 10 mice to allergic rhinitis-induced (ARE) without treatment group, and 10 mice to the allergic rhinitis-induced (FGT) after Douchi Hataedock treatment group. The 3-week-old mice of the FGT group were given one 10 mg/kg dose of Douchi Hataedock extract and resensitized to allergic antigens at weeks four, five, and six. Allergic rhinitis was induced primarily in mice nasal cavities for five days after one week of final sensitization. The second induction used the same method one week after the first induction was completed. After one week, the nasal mucosal tissues of each group were observed. Immunohistochemical staining for IL-4, STAT6, CD40, FcεRI, substance P, MMP-9, NF-κB p65, p-IkB, and iNOS in the nasal mucosa was also performed. Results: The FGT group had less respiratory epithelial damage and less mucin secretion in goblet cells than the ARE group and showed a 62% decrease in IL-4, 85% decrease in STAT6, 71% decrease in CD40, 69% decrease in FcɛRI, 43% decrease in substance P, 49% decrease in MMP-9, 43% decrease in NF-kB p65, 38% decrease in p-IkB, and 73% decrease in iNOS compared to the ARE group. Conclusions: Douchi Hataedock lessens inflammation in epithelial and goblet cells and reduces inflammatory mediator secretion in a mouse allergic rhinitis model.

참고문헌 (35)

  1. 고민정, "소아 알레르기비염 환자의 한방치료 효과에 대한 임상적 연구", 대한한방소아과학회지, 25(3), pp.12~26 (2011), 대한한방소아과학회
  2. David PS, "Allergic rhinits: definition, epidemiology, pathophysiology, detection, and diagnosis", J Allergy ClinImmunol, 108, pp.2~8 (2001)
  3. 김지은, "알레르기성 비염의 변증과 평가방법에 대한 고찰", 대한한방소아과학회지, 30(1), pp.32~39 (2016), 대한한방소아과학회
  4. "Allergic Rhinitis and its Impact on Asthma (ARIA) in collaboration with the World Health Organization, Global Allergy and Asthma European Network (GA2LEN)"[URL]
  5. 구은진, "알레르기성 비염의 한의학 및 보완대체의학적 치료에 대한 국내 임상 연구 동향", 대한한방소아과학회지, 29(4), pp.77~89 (2015), 대한한방소아과학회
  6. Jan L. Brożek, "Allergic Rhinitis and its Impact on Asthma (ARIA) guidelines—2016 revision", Journal of Allergy and Clinical Immunology, 140(4), pp.950~958 (2017), Elsevier BV[DOI]
  7. 김재숙, "소아 알레르기비염의 임상 특징", Allergy Asthma & Respiratory Diseases, 3(2), pp.116~123 (2015), 대한 소아알레르기 호흡기학회
  8. 우현수, "알레르기성 비염의 韓方치료에 대한 고찰", 대한한의학회지, 27(1), pp.155~164 (2006), 대한한의학회
  9. 김자혜, "알레르기 비염의 대체의학적 치료에 관한 최근 논문경향 분석 - Entrez Pubmed 위주", 한방안이비인후피부과학회지, 20(1), pp.239~249 (2007), 대한한방안이비인후피부과학회
  10. Leung DY, "Atopic dermatitis: new insights and opportunities for therapeutic intervention", J Allergy Clin Immunol, 105(5), pp.860~876 (2000)
  11. Mosmann TR, "Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins", J Immunol, 136(7), pp.2348~2357 (1986)
  12. Saini V, "Cytokines in recurrent pregnancy loss", Clinica Chimica Acta, 412(9-10), pp.702~708 (2011)
  13. Son JH, "Effects of Pal- Mul-Tang on Cytokines Production from Immune cells during the late stage of Pregnancy in BALB", c, 2000, pp.13(1), 342~375
  14. Jung AR, "Douchi (Glycine Semen Preparatum) alleviates atopic dermatitis-like skin lesions in NC", Nga mice by regulation of PKC and Th2 cytokines, 2016, pp.16, 416~
  15. 엄선호, "豆豉 추출물을 이용한 下胎毒法이 NC", Nga 생쥐에서 유발된 아토피 유도 피부염에 미치는 항염증 효과, 2016, pp.30(2), 1~9[DOI]
  16. 안상현, "두시 하태독법의 IL-4 활성 조절이 D. farinae 유도 아토피유사피부염 발병 조절에 미치는 효과", 대한한방소아과학회지, 31(1), pp.43~51 (2017), 대한한방소아과학회
  17. Kim JW, "Atopic dermatitis form allergy and immunological perspective", Kor J dermatology, 41(6), pp.687~689 (2003)
  18. 박용민, "아토피피부염 병태생리에 대한 최신 지견", Allergy Asthma & Respiratory Diseases, 16(3), pp.189~196 (2006), 대한 소아알레르기 호흡기학회
  19. Romagnani S, "Regulation of the development of type 2 T-helper cells in allergy", Curr Opin Immunol, 6(6), pp.838~846 (1994)
  20. Kidd P, "Th1", Th2 balance: the hypothesis, its limitations, and implications for health and disease, 2003, pp.8(3), 223~246
  21. Romagnani S, "T-cell subsets (Th1 versus Th2)", Ann Allergy Asthma Immunol, 85(1), pp.9~21 (2000)
  22. Akdis M, "IgE class switching and cellular memory", Nat Immunol, 13(4), pp.312~314 (2012)
  23. Leek JP, "Assignment of the STAT6 gene (STAT6) to human chromosome band 12q13 by in situ hybridization", Cytogenetics and Cell Genetics, 79(3-4), pp.208~209 (1997)
  24. Hou J, "An interleukin-4-induced transcription factor: IL-4 Stat", Science, 265(5179), pp.1701~1706 (1994)
  25. Amin K, "The role of mast cells in allergic inflammation", Respir Med, 106(1), pp.9~14 (2012)
  26. Metcalfe DD, "Mast cells", Physiol Rev, 77(4), pp.1033~1079 (1997)
  27. Stone KD, "IgE, Mast Cells, Basophils, and Eosinophils", J Allergy Clin Immunol, 125(2 Suppl 2), pp.S73~S80 (2010)
  28. Park SW, "Substance P is a promoter of adult neural progenitor cell proliferation under normal and ischemic conditions", J Neurosurg, 107(3), pp.593~599 (2007)
  29. Donkin JJ, "Substance P in traumatic brain injury", Prog Brain Res, 161, pp.97~109 (2007)
  30. Ebner K, "The role of substance P in stress and anxiety responses", Amino Acids, 31(3), pp.251~272 (2006)
  31. Yamaoka J, "Rapid changes in substance P signaling and neutral endopeptidase induced by skin-scratching stimulation in mice", J Dermatol Sci, 48(2), pp.123~132 (2007)
  32. Woessner JF Jr, "MMPs and TIMPs-an historical perspective", Mol Biotechnol, 22(1), pp.33~49 (2002)
  33. Burrage PS, "Matrix metalloproteinases ; role in arthritis", Front Biosci, 11, pp.529~543 (2006)
  34. Onodera S, "Macrophage migration inhibitory factor up-regulates expression of matrix metalloproteinases in synovial fibroblast of rheumatoid arthritis", J Biol Chem, 275(1), pp.444~450 (2000)
  35. Baeuerle PA, "NF-κB - Ten years after", Cell, 87, pp.13~20 (1996)

관련 논문AI추천

두시 하태독법이 시행된 비만 생쥐에서 Th2 분화조절을 통한 알러지성 비염 유발 감소 효과김기봉, 안상현대한한방소아과학회 · 대한한방소아과학회지2018 · 제32권 제2호알레르기성 비염에서 황련-감초 하태독법의 IL-4활성 조절을 통한 항염증효과정아람한의병리학회 · 동의생리병리학회지2019 · 제33권 제2호비만 유발 생쥐에서 Th2분화조절을 통한 황련-감초 하태독법의 알레르기성 비염 발현 억제효과김기봉, 안상현, 정아람대한한방소아과학회 · 대한한방소아과학회지2019 · 제33권 제2호두시 하태독법의 IL-4 활성 조절이 D. farinae 유도 아토피유사피부염 발병 조절에 미치는 효과김기봉, 김재규, 안상현, 천진홍대한한방소아과학회 · 대한한방소아과학회지2017 · 제31권 제1호알레르기 비염 유발 생쥐에 대한 荊芥連翹湯의 iNOS 생성 억제 효과박정훈, 홍승욱대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2012 · 제25권 제1호두시를 이용한 하태독법의 NC/Nga 생쥐에서 DNFB로 유발된 피부손상 완화 효과김기봉, 김호현, 박선영, 송지훈, 안상현, 천진홍한의병리학회 · 동의생리병리학회지2016 · 제30권 제2호

이 간행물의 다른 논문 (11건)

인체 유방암세포 MDA-MB-231에서 乾漆 추출물이 PI3K/AKT 신호경로를 통한 caspase 의존적 apoptosis 유발에 미치는 영향홍상훈, 박상은pp. 409-418본태성 떨림의 침치료 효과에 대한 메타분석손유진, 강기완, 정민정, 장인수, 정서윤pp. 419-432레이노병의 침치료에 대한 연구동향장인수, 전상우, 정민정, 김호pp. 433-442오령산의 고혈압 치료약물 가능성에 대한 개관선승호, 정민정, 윤지현, 최유민, 장인수, 김홍준, 강기완, 강자연pp. 443-454강작열감증후군 환자의 미각 이상과 脾氣虛證의 상관관계이정은, 박재우, 김진성pp. 455-467
원문 보기