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두시 하태독법의 IL-4 활성 조절이 D. farinae 유도 아토피유사피부염 발병 조절에 미치는 효과

The Effect of Douchi Hataedock Treatment for Dermatophagoides Farinae-Induced Atopic Dermatitis-like Skin Lesions by Controlling IL-4 Activity

김기봉|천진홍|김재규|안상현
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2017.02|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제31권 제1호|pp. 43-51

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참고문헌

키워드

Atopic dermatitisIL-4DouchiFc ε recetorSubstance PHataedockNF-k
주제분류한의학

초록

Objectives Hataedock method is a Korean medical therapy which removes fetal toxin by orally administering herbal decoction to neonates. This study was to observe skin damage and anti-inflammatory effect via regulating IL-4 activity in NC/Nga mice which were induced atopic dermatitis (AD)-like skin lesion by Dermatophagoides (D.) farinae after applying Douchi Hataedock method. Methods NC/Nga mice with 3 weeks of gestational age were used. Each 10 mice were allocated to the control group (Ctrl), the AD-induced group (AE), and the group which induced AD after administering Douchi extract (GT). After 4 weeks from administering Douchi extract to the mice, the primary AD was induced by applying D. farinae extract 6 times per week for 3 weeks and then the secondary AD was induced by the same method after 1 week from the primary AD induction. To identify the skin damage and anti-inflammatory effect, we observed LxR, IL-4, Fc ε receptor, substance P, and NF-κB. Results The GT group showed alleviation of skin injury and decrease in capillary angiogenesis. Stratum corneum damage, epithelial cell hyperplasia, lymphocyte infiltration, and capillary distribution relatively decreased in the GT group. LxR-positive reaction in the GT group were increased by 53% than that of the AE group. IL-4 production, Fc ε receptor activity, and substance P-positive reaction in the GT group were decreased by 82%, 42%, and 82% respectively compare to those of the AE group. NF-κB-positive reaction in the GT group were decreased by 15% compare to that of the AE group. Conclusions Hataedock method with Douchi extract alleviated AD via reducing inflammatory cytokines secreted at the early stage of AD. Thus, Douchi Hataedock method has a beneficial effect for the prevention and treatment of AD.

Abstract

Objectives Hataedock method is a Korean medical therapy which removes fetal toxin by orally administering herbal decoction to neonates. This study was to observe skin damage and anti-inflammatory effect via regulating IL-4 activity in NC/Nga mice which were induced atopic dermatitis (AD)-like skin lesion by Dermatophagoides (D.) farinae after applying Douchi Hataedock method. Methods NC/Nga mice with 3 weeks of gestational age were used. Each 10 mice were allocated to the control group (Ctrl), the AD-induced group (AE), and the group which induced AD after administering Douchi extract (GT). After 4 weeks from administering Douchi extract to the mice, the primary AD was induced by applying D. farinae extract 6 times per week for 3 weeks and then the secondary AD was induced by the same method after 1 week from the primary AD induction. To identify the skin damage and anti-inflammatory effect, we observed LxR, IL-4, Fc ε receptor, substance P, and NF-κB. Results The GT group showed alleviation of skin injury and decrease in capillary angiogenesis. Stratum corneum damage, epithelial cell hyperplasia, lymphocyte infiltration, and capillary distribution relatively decreased in the GT group. LxR-positive reaction in the GT group were increased by 53% than that of the AE group. IL-4 production, Fc ε receptor activity, and substance P-positive reaction in the GT group were decreased by 82%, 42%, and 82% respectively compare to those of the AE group. NF-κB-positive reaction in the GT group were decreased by 15% compare to that of the AE group. Conclusions Hataedock method with Douchi extract alleviated AD via reducing inflammatory cytokines secreted at the early stage of AD. Thus, Douchi Hataedock method has a beneficial effect for the prevention and treatment of AD.

참고문헌 (18)

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두시를 이용한 하태독법의 NC/Nga 생쥐에서 DNFB로 유발된 피부손상 완화 효과김기봉, 김호현, 박선영, 송지훈, 안상현, 천진홍한의병리학회 · 동의생리병리학회지2016 · 제30권 제2호豆豉 추출물을 이용한 下胎毒法이 NC/Nga 생쥐에서 유발된 아토피 유도 피부염에 미치는 항염증 효과김기봉, 박선영, 안상현, 엄선호, 천진홍대한한방소아과학회 · 대한한방소아과학회지2016 · 제30권 제2호PKC 활성 조절을 통한 두시 하태독법의 항염증작용이 Mite 항원 유도 아토피유사피부염 발병 조절에 미치는 효과김기봉, 안상현대한한방소아과학회 · 대한한방소아과학회지2016 · 제30권 제4호하태독법 중 황련감초법이 DNFB로 유발된 NC/Nga 생쥐의 아토피 피부염에 미치는 영향김기봉, 박선영, 안상현, 정아람, 차호열, 천진홍대한한방소아과학회 · 대한한방소아과학회지2015 · 제29권 제4호Hataedock Treatments for Dermatophagoides Farinae-induced Atopic Dermatitis in NC/Nga Mice Treated with High-fat Diet김기봉, 김희연, 안상현, 양인준, 정한솔한의병리학회 · 동의생리병리학회지2018 · 제32권 제6호황련-감초 추출물을 이용한 하태독법이 NC/Nga 생쥐에서 유발된 아토피 유사 피부염에 미치는 항염증 효과김기봉, 박선영, 안상현, 정아람, 차호열, 천진홍, 최준용대한한방내과학회 · 대한한방내과학회지2015 · 제36권 제4호

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