Response of broiler chickens to diets containing different levels of sodium with or without microbial phytase supplementation
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초록
Phytate induced excessive mineral excretion through poultry litter leads to poor performance and environmental pollution. Exogenous microbial phytase supplementation to poultry diets reduce the environmental excretion of nutrient and improve bird’s performance. However, excessive dietary sodium (Na) level may hinder the phytase-mediated phytate hydrolysis and negate the beneficial effects of phytase. Therefore, this experiment was conducted to investigate the effects of different concentration dietary Na on phytase activity and subsequent impact on broiler performance, bone mineralisation and nutrient utilisation. In this study, six experimental diets, consisting of three different levels of Na (1.5, 2.5, or 3.5 g/kg) and two levels of microbial phytase (0 or 500 U/kg) were formulated by using 3 × 2 factorial design. The six experimental diets were offered to 360 day-old Ross 306 male chicks for 35 days, where, each experimental diet consisted of 6 replicates groups with 10 birds. Along with growth performance, nutrient utilization, intestinal enzyme activity, dry matter (DM) content of litter and mineral status in bone were analysed. Dietary Na and phytase had no effect on bode weight gain and feed intake. Birds on the low Na diet showed higher (p < 0.05) feed conversion ratio (FCR) than the mid-Na diets. High dietary Na adversely affected (p < 0.001) excreta DM content. Phytase supplementation to the high-Na diet increased (p < 0.01) the litter ammonia content. High dietary Na with phytase supplementation improved (Na × phytase, p < 0.05) the AME value and ileal digestibility of Ca and Mg. The total tract retention of Ca, P, and Mg was reduced with high Na diet, which was counteracted by phytase supplementation (Na × phytase, p < 0.001). The diets containing mid-level of Na improved (p < 0.001) the function of Na-KATPase and Mg-ATPase in the jejunum. The overall results indicate that high dietary Na did not affect phytase activity but influenced the nutrient utilization of birds, which was not reflected in bird overall performance.
Abstract
Phytate induced excessive mineral excretion through poultry litter leads to poor performance and environmental pollution. Exogenous microbial phytase supplementation to poultry diets reduce the environmental excretion of nutrient and improve bird’s performance. However, excessive dietary sodium (Na) level may hinder the phytase-mediated phytate hydrolysis and negate the beneficial effects of phytase. Therefore, this experiment was conducted to investigate the effects of different concentration dietary Na on phytase activity and subsequent impact on broiler performance, bone mineralisation and nutrient utilisation. In this study, six experimental diets, consisting of three different levels of Na (1.5, 2.5, or 3.5 g/kg) and two levels of microbial phytase (0 or 500 U/kg) were formulated by using 3 × 2 factorial design. The six experimental diets were offered to 360 day-old Ross 306 male chicks for 35 days, where, each experimental diet consisted of 6 replicates groups with 10 birds. Along with growth performance, nutrient utilization, intestinal enzyme activity, dry matter (DM) content of litter and mineral status in bone were analysed. Dietary Na and phytase had no effect on bode weight gain and feed intake. Birds on the low Na diet showed higher (p < 0.05) feed conversion ratio (FCR) than the mid-Na diets. High dietary Na adversely affected (p < 0.001) excreta DM content. Phytase supplementation to the high-Na diet increased (p < 0.01) the litter ammonia content. High dietary Na with phytase supplementation improved (Na × phytase, p < 0.05) the AME value and ileal digestibility of Ca and Mg. The total tract retention of Ca, P, and Mg was reduced with high Na diet, which was counteracted by phytase supplementation (Na × phytase, p < 0.001). The diets containing mid-level of Na improved (p < 0.001) the function of Na-KATPase and Mg-ATPase in the jejunum. The overall results indicate that high dietary Na did not affect phytase activity but influenced the nutrient utilization of birds, which was not reflected in bird overall performance.
참고문헌 (32)
- Borges SA, "Dietary electrolyte balance for broiler chickens exposed to thermoneutral or heat-stress environments", Poult Sci, 82, pp.428~435 (2003)
- Mongin P., "Recent advances in dietary anion-cation balance : applications in poultry", Proc Nutr Soc, 40, pp.285~294 (1981)
- Gal-Garber O, "Nutrient trans-port in the small intestine : Na+K+-ATPase expression and activity in the small intestine of the chicken as influenced by dietary sodium", Poult Sci, 82, pp.1127~1133 (2003)
- Selle PH, "Protein-phytate interactions in pig and poultry nutrition : a reappraisal", Nutr Res Rev, 25, pp.1~17 (2012)
- Vieira SL, "Sodium requirements for the first seven days in broiler chicks", J Appl Poult Res, 12, pp.362~370 (2003)
- National Research Council, "Nutrient requirements of poultry" (1994), National Academy Press
- Selle PH, "Microbial phytase in poultry nutrition", Anim Feed Sci Technol, 135, pp.1~41 (2007)
- Akter M, "Interactions between phytase and different dietary minerals in in vitro systems", J Food Agric Environ, 13, pp.38~44 (2015)
- Cowieson AJ, "The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens", Br Poult Sci, 45, pp.101~108 (2004)
- Ravindran V, "Influence of an Escherichia coli-derived phytase on nutrient utilization in broiler starters fed diets containing varying concentrations of phytic acid", Poult Sci, 85, pp.82~89 (2006)
- Adeola O, "Board-invited review : opportunities and challenges in using exogenous enzymes to improve nonruminant animal production", J Anim Sci, 89, pp.3189~3218 (2011)
- Ravindran V, "Influence of dietary electrolyte balance and microbial phytase on growth performance, nutrient utilization, and excreta quality of broiler chickens", Poult Sci, 87, pp.677~688 (2008)
- Pos J, "Effect of phytase and dietary calcium level on litter quality and broiler performance", Proceeding of the 14th European Symposium on Poultry Nutrition (2003)
- Akter M, "Response of broiler chickens to different levels of calcium, non-phytate phosphorus and phytase", Br Poult Sci, 57, pp.799~809 (2016)
- "Ross 308 broiler: nutrition specification" (2014)[URL]
- Akter MM, "Influence of different levels of calcium, non-phytate phosphorus and phytase on apparent metabolizable energy, nutrient utilization, plasma mineral concentration and digestive enzyme activities of broiler chickens", J Appl Anim Res, 46, pp.278~286 (2018)
- Kageyama N., "A direct colorimetric determination of uric acid in serum and urine with uricase-catalase system", Clin Chim Acta, 31, pp.421~426 (1971)
- Oviedo-Rondon EO, "Sodium and chloride requirements of young broiler chickens fed corn-soybean diets(one to twenty-one days of age)", Poult Sci, 80, pp.592~598 (2001)
- Mirza Muhammad Haroon Mushtaq, "Carcass and body organ characteristics of broilers supplemented with dietary sodium and sodium salts under a phase feeding system", 한국축산학회지, 56(1), pp.1~7 (2014), 한국축산학회
- Goodgame SD, "Effect of phytase on the sodium requirement of starting broilers 2. Sodium chloride as sodium source", Int J Poult Sci, 10, pp.766~773 (2011)
- Bedford MR, "Assessing measurements in feed enzyme research : phytase evaluations in broilers", J Appl Poult Res, 25, pp.305~314 (2016)
- Hernandez F, "Effect of dietary protein level on retention of nutrients, growth performance, litter composition and NH3 emission using a multi-phase feeding programme in broilers", Span J Agric Res, 11, pp.736~746 (2013)
- Pereira Goncalves R., "Influence of dietary electrolyte balance on phytase efficacy in poultry", MRes, pp.2014
- Cowieson AJ, "Phytic acid and phytase : implications for protein utilization by poultry", Poult Sci, 85, pp.878~885 (2006)
- Selle PH, "Beneficial effects of xylanase and", or phytase inclusions on ileal amino acid digestibility, energy utilisation, mineral retention and growth performance in wheat-based broiler diets, 2009, pp.153, 303~313
- Truong HH, "Standard phytase inclusion in maize-based broiler diets enhances digestibility coefficients of starch, amino acids and sodium in four small intestinal segments and digestive dynamics of starch and protein", Anim Feed Sci Technol, 209, pp.240~248 (2015)
- Truong HH, "Phytase supplementation of maize-, sorghumand wheat-based broiler diets with identified starch pasting properties influences phytate(IP6)and sodium jejunal and ileal digestibility", Anim Feed Sci Technol, 198, pp.248~256 (2014)
- Bye JW, "Dual effects of sodium phytate on the structural stability and solubility of proteins", J Agric Food Chem, 61, pp.290~295 (2013)
- Liu N, "Effect of diet containing phytate and phytase on the activity and messenger ribonucleic acid expression of carbohydrase and transporter in chickens", J Anim Sci, 86, pp.3432~3439 (2008)
- Therein AG, "Mechanisms of sodium pump regulation", Am J Physiol Cell Physio, 279, pp.C541~C566 (2000)
- Sklan D, "Hydrolysis and absorption in the small intestines of posthatch chicks", Poult Sci, 79, pp.1306~1310 (2000)
- Dersjant-Li Y, "Phytase in non-ruminant animal nutrition : a critical review on phytase activities in the gastrointestinal tract and influencing factors", J Sci Food Agric, 95, pp.878~896 (2015)