붕장어 (Conger myriaster) 의 뇌로부터 Vasopressin-related Peptide, [Arg8]-vasotocin의 정제
Purification of Vasopressin-related Peptide, [Arg8]-vasotocin, from the Brain of Conger Eel (Conger myriaster)
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Abstract
Vasopressin (VP)-related peptide was purified from the brain extract of conger eel (Conger myriaster) by reverse-phase, ion-exchange high performance liquid chromatography (HPLC). This peptide with a molecular weight of 1,051.2Da was determined as H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Arg-Gly-NH2, whose Cys residues made an intramolecular disulfide bridge by the automated amino acid sequence analysis, MALDI-TOF mass spectrometry. It's sequence was confirmed by identity of the elution position with the synthetic peptide in HPLC system. As a result of homology investigation, the primary structure of this peptide was the same as that of VP-superfamily member, [Arg8]-vasotocin. The synthetic peptide showed a contractile activity at a minimal effective concentration of 10-10M on the intestinal smooth muscle of goldfish.
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