EST-based Identification of Genes Expressed in the Brain of the Olive Flounder Paralichthys olivaceus
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
We have constructed a cDNA library using brain samples of olive flounder Paralichthys olivaceus. Here, we described the study on gene identification by screening 356 clones from the brain cDNA library of olive flounder. Here, we screened 356 clones from the library to identify genes. Of these, 176 (49.5%) were identified as orthologs of known genes from olive flounder and other organisms. Among the 176 EST clones, 33 (18.7%) represented 11 unique genes that are identical to expressed sequence tags (ESTs) reported for olive flounder, and 120 (68.2%) represented 102 unique genes known from other organisms. The percentage of unknown genes (50.5%) is higher than in other olive flounder cDNA libraries (Lee et al., 2003, 2006, 2007), reflecting the high complexity of brain tissue. Further studies of expression characterization and developmental behavior related to these genes should provide useful insight into the physiological functions of the brain in olive flounder.
Abstract
We have constructed a cDNA library using brain samples of olive flounder Paralichthys olivaceus. Here, we described the study on gene identification by screening 356 clones from the brain cDNA library of olive flounder. Here, we screened 356 clones from the library to identify genes. Of these, 176 (49.5%) were identified as orthologs of known genes from olive flounder and other organisms. Among the 176 EST clones, 33 (18.7%) represented 11 unique genes that are identical to expressed sequence tags (ESTs) reported for olive flounder, and 120 (68.2%) represented 102 unique genes known from other organisms. The percentage of unknown genes (50.5%) is higher than in other olive flounder cDNA libraries (Lee et al., 2003, 2006, 2007), reflecting the high complexity of brain tissue. Further studies of expression characterization and developmental behavior related to these genes should provide useful insight into the physiological functions of the brain in olive flounder.
참고문헌 (27)
- Adams MD, "Complementary DNA se-uenceing: expressed sequence tags and human genome project", Science, 252, pp.1651~1656 (1991)
- Aoki T, "Sequences of 596 cDNA (565,977 bp) clones of Japanese flounder (Paralichthys olivaceus) leukocytes infected with hirame rhabdovirus", Mar Biotechnol, 1, pp.477~488 (1999)
- Chen SL, "Analysis of immunerelevant genes expressed in red sea bream (Chrysophrys major) spleen", Aquaculture, 240, pp.115~130 (2004)
- Chomczynski P, "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction", Analyt Biochem, 162, pp.156~159 (1987)
- Davey GC, "A survey of genes in Atlantic salmon (Salmo salar) as identified by expressed sequence tags", Gene, 263, pp.121~130 (2001)
- Douglas SE, "Winter flounder expressed sequence tags: establishment of an EST database and identification of novel fish genes", Mar Biotechnol, 1, pp.458~464 (1999)
- Gawantka V, "Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterning", Mech Dev, 77, pp.95~141 (1998)
- Ju Z, "Transcriptome analysis of channel catfish (Ictalurus punctatus): genes and expression profile from the brain", Gene, 261, pp.373~382 (2000)
- Karsi A, "Trans-riptional activities in the pituitaries of channel catfish before and after induced ovulation by injection of carp pituitary extract as revealed by expressed se-uence tag analysis", J Mol Endocrinol, 21, pp.121~129 (1998)
- Karsi A, "Transcriptome analysis of channel catfish (Ictalurus punctatus): initial analysis of gene expression and microsatellite-containing cDNAs in the skin", Gene, 285, pp.157~168 (2002)
- Kim S, "The skeletal muscle alpha-actin gene of channel catfish (Ictalurus punctatus) and its association with piscine specific SINE elements", Gene, 252, pp.173~181 (2000)
- Kimura T, "Large-scale isolation of ESTs from medaka embryos and its application to medaka developmental genetics", Mech. Dev., 121, pp.915~932 (2004)
- Kocabas MA, "Expression profile of the channel catfish spleen: analysis of genes involved in immune functions", Mar Biotechnol, 4, pp.526~536 (2002)
- Kono K, "The analysis of expressed genes in head kidney of common carp Cyprinus carpio L. stimulated with peptidoglycan", Aquaculture, 235, pp.37~52 (2004)
- Jeong-HoLee, "Expressed Sequence Tag Analysis of Olive Flounder (Paralichthys olivaceus): Genes and Expression Profile from the Liver", Fisheries and Aquatic Sciences, 6(3), pp.110~115 (2003), 한국수산과학회
- 이정호, "넙치 (Paralichthys olivaceus) 신장에서 생성된 ESTs (Expressed Sequence Tags)로부터 면역 관련 유전자의 탐색", 한국어류학회지, 18(4), pp.283~292 (2006), 한국어류학회
- 이정호, "EST (Expressed sequence tag)를 통한 넙치 (Paralichthys olivaceus) 정소의 유전자 발현 패턴 분석", 한국어류학회지, 19(4), pp.257~265 (2007), 한국어류학회
- Martin SA, "EST-based identification of genes expressed in the liver of adult Atlantic salmon (Salmo salar)", Biochem Biophys Res Commun, 293, pp.578~585 (2002)
- Matoba R, "Correlation between gene functions and developmental expression patterns in the mouse cerebellum", Eur J Neurosci, 12, pp.1357~1371 (2000)
- Nam BH, "A survey of expressed genes in the leukocytes of Japanese flounder, Paralichthys olivaceus, infected with hirame rhabdovirus", Dev Comp Immunol, 24, pp.13~24 (2000)
- Nam BH, "Bulk isolation of immune response-related genes by expressed sequen-ed tags of Japanese flounder Paralichthys olivaceus leucocytes stimulated with Con A", PMA, 2003, pp.14, 467~476
- Parsons JD, "Improved tools for DNA comparison and clustering", Comp Appl Biosci, 11, pp.603~613 (1995)
- Sambrook J, "Frisch EF and Maniatis T. 1989. Molecular Cloning, A Laboratory Manual" (1989), Cold Spring Harbor Laboratory Press
- Sarropoulou E, "Comparative analysis and charac-erization of expressed sequence tags in gilthead sea bream (Sparus aurata) liver and embryos", Aqua-ulture, 243, pp.69~81 (2005)
- Shiue YL, "EST based identification of genes expressed in the hypothalamus of adult tilapia, Oreochromis mossambicus", Biochem Biophys Res Commun, 316, pp.523~527 (2004)
- Ton C, "Identifi-ation, characterization and mapping of expressed sequence tags from an embryonic zebrafish heart cDNA library", Genome Res, 10, pp.1915~1927 (2000)
- Wen X, "Large-scale tem-oral gene expression mapping of central nervous system development", Proc Natl Acad Sci USA, 95, pp.334~339 (1998)