Is Koi Herpesvirus (KHV) Related to the Mass Mortality Occurring among Cultured Carp, Cyprinus carpio, in Korea?
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Since 1998, a new viral disease with high mortality has been consistently recorded in Korea in cultured carp, Cyprinus carpio. In this study, we investigated an epizootic of the disease that caused high mortality rates in carp obtained from 11 farms in Korea between 1999 and 2007. Assessment of koi herpesvirus (KHV) levels in diseased carp was carried out to determine if this virus was the etiologic agent of disease in this instance. High mortality rates in carp were recorded mainly in the spring and autumn at water temperatures between 19°C and 24°C. Diseased fish typically showed surface discoloration, with a thick opaque mucus covering the body and gills. Protozoan parasites and bacteria were recovered from 7/29 (24%) and 2/26 (8%) of fish, respectively. Evidence of viral infection was marked; cytopathic effects (CPEs), characterized by cell rounding and an extended cytoplasm in fathead minnow (FHM) cells, were detected in 40/41 fish (98%). A high mortality rate (80%) resulted when supernatants of cell cultures showing CPEs were applied to previously healthy fish. KHV was detected by polymerase chain reaction in 6/41 fish (15%), but was not detected in supernatants obtained from cell cultures showing CPEs. These results suggest that KHV may not be the etiologic agent of the high mortality occurring among cultured carp in Korea; therefore, some other—as yet unidentified—infective agent must be responsible.
Abstract
Since 1998, a new viral disease with high mortality has been consistently recorded in Korea in cultured carp, Cyprinus carpio. In this study, we investigated an epizootic of the disease that caused high mortality rates in carp obtained from 11 farms in Korea between 1999 and 2007. Assessment of koi herpesvirus (KHV) levels in diseased carp was carried out to determine if this virus was the etiologic agent of disease in this instance. High mortality rates in carp were recorded mainly in the spring and autumn at water temperatures between 19°C and 24°C. Diseased fish typically showed surface discoloration, with a thick opaque mucus covering the body and gills. Protozoan parasites and bacteria were recovered from 7/29 (24%) and 2/26 (8%) of fish, respectively. Evidence of viral infection was marked; cytopathic effects (CPEs), characterized by cell rounding and an extended cytoplasm in fathead minnow (FHM) cells, were detected in 40/41 fish (98%). A high mortality rate (80%) resulted when supernatants of cell cultures showing CPEs were applied to previously healthy fish. KHV was detected by polymerase chain reaction in 6/41 fish (15%), but was not detected in supernatants obtained from cell cultures showing CPEs. These results suggest that KHV may not be the etiologic agent of the high mortality occurring among cultured carp in Korea; therefore, some other—as yet unidentified—infective agent must be responsible.
참고문헌 (10)
- Gilad O, "Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, Cyprinus carpio koi", Dis Aquat Org, 48, pp.101~108 (2002)
- Haenen O, "The emergence of koi herpesvirus and its significance to European aquaculture", Bull Eur Assoc Fish Pathol, 24, pp.293~307 (2004)
- Hedrick RP, "A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carp", J Aquat Anim Health, 12, pp.44~57 (2000)
- Hutoran M, "Description of an as yet unclassified DNA virus from diseased Cyprinus carpio species", J Virol, 79, pp.1983~1991 (2005)
- Miyazaki T, "Histopathological and ultra-structural features of koi herpesvirus (KHV)-infected carp Cyprinus carpio, and the morphology and morphogenesis of KHV", Dis Aquat Org, 80, pp.1~11 (2008)
- Oh MJ, "A viral disease occurring in cultured carp Cyprinus carpio in Korea", Fish Pathol, 36, pp.147~151 (2001)
- Pokorova D, "Current knowledge on koi her-pesvirus (KHV): a review", Vet Med-Czech, 50, pp.139~147 (2005)
- Sano M, "First detection of koi herpesvirus in cultured common carp Cyprinus carpio in Japan", Fish Pathol, 39, pp.165~167 (2004)
- Seo JW, "Histopathology of unidentified viral disease of cultured carp, Cyprinus carpio", J Fish Pathol sub-mitted (2010)
- Waltzek TB, "Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae", J Gen Virol, 86, pp.1659~1667 (2005)