서해산 일반 꽃게와 보라색 변이 꽃게의 계측형질 및 AFLP 분석에 의한 집단 구조
Population Structure of the Blue and Purple Types of the Blue Crab Portunus trituberculatus (Miers) from the West Sea of Korea Based on Morphometric Characteristics and AFLP Analysis
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In Korean waters, there are two color types (blue and purple) of the blue crab Portunus trituberculatus. The blue type is common, but the ratio of the purple type has increased in landings. To determine whether there were significant morphometric or genetic differences between the blue and purple types, crabs caught from the West Sea of Korea were examined. Based on covariance analysis, there were significant differences in 1 of 10 morphometric characteristics of males between the two types, in none of the ten characteristics for females. Using amplified fragment length polymorphism (AFLP) DNA fingerprinting, no specific AFLP marker was detected for each type. The heterozygosity and genetic diversity were very low. Analyses of pairwise distance, the Fst index, and genetic similarity revealed similar results, with very low genetic differentiation. Therefore, there is no significant difference between blue and purple types of the crab from the West Sea of Korea, and the two types in the West Sea can be managed as one stock.
Abstract
In Korean waters, there are two color types (blue and purple) of the blue crab Portunus trituberculatus. The blue type is common, but the ratio of the purple type has increased in landings. To determine whether there were significant morphometric or genetic differences between the blue and purple types, crabs caught from the West Sea of Korea were examined. Based on covariance analysis, there were significant differences in 1 of 10 morphometric characteristics of males between the two types, in none of the ten characteristics for females. Using amplified fragment length polymorphism (AFLP) DNA fingerprinting, no specific AFLP marker was detected for each type. The heterozygosity and genetic diversity were very low. Analyses of pairwise distance, the Fst index, and genetic similarity revealed similar results, with very low genetic differentiation. Therefore, there is no significant difference between blue and purple types of the crab from the West Sea of Korea, and the two types in the West Sea can be managed as one stock.
참고문헌 (35)
- Aarts, H.J., "High- resolution genotyping of Salmonella strains by AFLP-fingerprinting", Lett. Appl. Microbiol, 26, pp.131~135 (1998)
- Blears, M.J., "Amplified fragment length polymorphism (AFLP): a review of the procedure and its applications", J. Ind. Microbiol. Biotechnol, 21, pp.99~114 (1998)
- Bonin, A., "Statistical analysis of amplified fragment length polymorphism data: a toolbox for molecular ecologists and evolutionists", Mol. Ecol, 16, pp.3737~3758 (2007)
- Carlson,S.P, "The color changes in Uca pugilator", Zoology, 21, pp.549~551 (1935)
- Cushing,D.H, "Fisheries Biology, A Study in Population Dynamics", pp.1~200 (1968), University of Wisconsin Press, Madison, WI, USA
- Felder, D.L, "Genetic differentiation in trans-Floridian species complexes of Sesarma and Uca (Decapoda: Brachyura)", J. Crust. Biol, 14, pp.191~209 (1994)
- Huxley,J, "Problems of Relative Growth", pp.1~276 (1932), London, UK
- Jones, C.J., "Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories", Mol. Breed, 3, pp.381~390 (1997)
- Kim, H.S, "A Catalog of Anomura and Brachyura from Korea. Encyclopedia of Fauna & Flora of Korea", Anomura and Brachyura. Samwha Co, 14, pp.1~694 (1973), Seoul, Korea
- Kim, I.H, "Korean barnacles (Crustaces, Cirriprdia, Thoracia)", 박사, pp.1985
- Kim, Y.M, "On the result of morphological measurement of the red sea bream, Pagrus major in the southern and western coast of Korea, Rep", Fish. Res, 8, pp.103~111 (1970)
- Knorr, C., "Application of AFLP markers to genome mapping in poultry.Anim.Genet.,30,28-35", Anim. Genet, 30, pp.28~35 (1999)
- Li, Z., "The effects of selective breeding on the genetic structure of shrimp Fenneropenaeus chinensis populations", Aquaculture, 258, pp.278~282 (2006)
- Mackill, D.J., "Level of polymorphism and genetic mapping of AFLP markers in rice", Genome, 39, pp.969~977 (1996)
- Mariette, S., "Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite marker", Heredity, 86, pp.469~479 (2001)
- McGaw, I.J, "Salinity preference of the shore crab, Carcinus maenas in relation to coloration during intermoult and to prior acclimation", J. Exp. Mar. Biol. Ecol, 155, pp.145~159 (1992)
- Melville-Smith, R., "Factors affecting colour change in 'white' western rock lobsters, Panulirus cygnus", J. Exp. Mar. Biol. Ecol, 291, pp.111~129 (2003)
- "Tools for population genetic analysis (TFPGA) 1.3: a windows programme for the sanalysis of allozyme and molecular population genetic data"[URL]
- MOMAF, "Statistical Yearbook of Maritime Affair and Fisheries 2003-2207", Ministry of Maritime and Fisheries, Korea
- Nei, M, "Mathematical model for studing genetical variation in terms of restriction endonucleases", Proc. Natl. Acad. Sci, 74, pp.5267~5273 (1979), USA
- Park, C.S, "Morphormetric comparison of herring, Clupea pallasi Cuvier et Valenciennes, between western waters and eastern waters of Korea", Bull. Fish. Res. Dev. Agency, 27, pp.103~109 (1981)
- Reineke, A, "Simplified AFLP protocol: replacement of primer labeling by the incorporation of alpha-labeled nucleotides during PCR", Biotechniques, 28, pp.622~623 (2000)
- Roa, A.C., "AFLP analysis of relationships among cassava and other Manihot species", Theor. Appl. Genet, 95, pp.741~746 (1997)
- Rusell, J.R., "Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs, and RAPDs", Theor. Appl. Genet, 95, pp.714~722 (1997)
- Sakai,T, "Studies on the Crabs of Japan. IV. Brachygnatha. Brachyrhyncha. Sci. Rep. Tokyo Bunrika Daigaku, Sect. 2B", 365~374 (1939)
- Snedecar, G.W, "Statistical Method", pp.1~507 (1980), Iowa State University Press, Ames, IO, USA
- Stefan, S., "Arlequin ver. 2.000: a software for population genetics data analysis" (2000), Genetics and Biometry Laboratory, Uni- versity of Geneva, Switzerland
- 성용길, "대하 Fenneropenaeus chinensis 집단의 AFLP 지문에 의한 유전 다양성 및 변이", 한국양식학회지, 20(4), pp.255~259 (2007), 한국양식학회
- Vos, P., "AFLP,a new technique for DNA fingerprinting.Nucl.Acids Res.,23,4407-4414", Nucl. Acids Res, 23, pp.4407~4414 (1995)
- Wang, Z.Y., "Applications of AFLP technology in genetic and phylogenetic analysis of penaeid shrimp", Biochem. System. Ecol, 32, pp.399~407 (2003)
- Wright, S, "The genetic structure of populations", Ann. Eugen, 15, pp.313~354 (1951)
- Yeon, I.J, "Fishery biology of the Blue crab, Portunus trituberculatus (Miers) in the West Sea of Korea and the East China Sea", qkrtk, pp.1997
- Yeon, I.J., "Compara- tive morphometric characteristics and commensal barnacles of the blue crab, Portunus trituberculatus (Miers) in the western coast of Korea and the East China Sea", Bull. Natl. Fish. Res. Dev. Agency, 46, pp.53~68 (1992)
- Zar,J.H, "Biostatistical Analysis", pp.1~663 (1999), Prentice- Hall International, Inc., Upper Saddle River, NJ, USA
- Zhang, C.I, "Fisheries Resources Ecolog", pp.1~399 (1991), Wooseong Co., Seoul, Korea