Isolation of a Novel Tenacibaculum sp. JS-1 and Characterization of Its β-Agarase
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This study reports the isolation of a bacterium capable of degrading agar and the characterization of its agarase. An agar-degrading marine bacterium JS-1 was isolated using Marine agar 2216 media from seawater collected from the seashore of Angolpo, Changwon, Gyeongnam Province, Republic of Korea. An agardegrading bacterium was named as Tenacibaculum sp. JS-1 by phylogenetic analysis based on 16S rRNA gene sequence. The extracellular crude agarase was prepared from the culture media of Tenacibaculum sp. JS-1 and used for characterization. Relative activities at 20, 30, 40, 50, and 60℃ were 39, 73, 100, 74, and 53%, respectively. Relative activities at pH 5, 6, 7, and 8 were 46%, 67%, 100%, and 49%, respectively. Its extracellular agarase showed maximum activity (164 U/l) at pH 7.0 and 40℃ in a 20 mM GTA buffer. The residual activities after heat treatment at 20, 30, and 50℃ for 30 min were 84, 73, and 26% or more, respectively. After 2 h heat treatment at 20, 30, 40, and 50℃, the residual activities were 80, 64, 52 and 21%, respectively. Thin layer chromatography analysis suggested that Tenacibaculum sp. JS-1 produces extracellular β-agarases that hydrolyze agarose to produce neoagarooligosaccharides, including neoagarohexaose (12.3%), neoagarotetraose (65.1%), and neoagarobiose (22.6%) at 6 h. Tenacibaculum sp. JS-1 and its β-agarase could be valuable for producing neoagarooligosaccharides with a variety of functional properties. These properties include inhibiting bacterial growth, slowing down starch degradation, and whitening, which are of interest for pharmaceuticals, food, cosmeceuticals, and nutraceuticals.
Abstract
This study reports the isolation of a bacterium capable of degrading agar and the characterization of its agarase. An agar-degrading marine bacterium JS-1 was isolated using Marine agar 2216 media from seawater collected from the seashore of Angolpo, Changwon, Gyeongnam Province, Republic of Korea. An agardegrading bacterium was named as Tenacibaculum sp. JS-1 by phylogenetic analysis based on 16S rRNA gene sequence. The extracellular crude agarase was prepared from the culture media of Tenacibaculum sp. JS-1 and used for characterization. Relative activities at 20, 30, 40, 50, and 60℃ were 39, 73, 100, 74, and 53%, respectively. Relative activities at pH 5, 6, 7, and 8 were 46%, 67%, 100%, and 49%, respectively. Its extracellular agarase showed maximum activity (164 U/l) at pH 7.0 and 40℃ in a 20 mM GTA buffer. The residual activities after heat treatment at 20, 30, and 50℃ for 30 min were 84, 73, and 26% or more, respectively. After 2 h heat treatment at 20, 30, 40, and 50℃, the residual activities were 80, 64, 52 and 21%, respectively. Thin layer chromatography analysis suggested that Tenacibaculum sp. JS-1 produces extracellular β-agarases that hydrolyze agarose to produce neoagarooligosaccharides, including neoagarohexaose (12.3%), neoagarotetraose (65.1%), and neoagarobiose (22.6%) at 6 h. Tenacibaculum sp. JS-1 and its β-agarase could be valuable for producing neoagarooligosaccharides with a variety of functional properties. These properties include inhibiting bacterial growth, slowing down starch degradation, and whitening, which are of interest for pharmaceuticals, food, cosmeceuticals, and nutraceuticals.
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