射干麻黃湯 및 <石室秘錄＞逆醫法方이 호흡기 점액의 분비에 미치는 영향
The Effects of Saganmahwang-tang and prescription C on airway mucin secretion
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Abstract
Objectives : This study was done to investigate whether two oriental prescriptions, saganmahwang-tang (SMT) and prescription C (P-C) significantly affect mucin release from cultured hamster tracheal surface epithelial (HTSE) cells. Methods : Confluent HTSE cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of SMT or P-C to assess the effect of each agent on 3H-mucin release. Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase (LDH) release. Also, the effects of SMT and P-C on contractility of isolated tracheal smooth muscle were investigated. Results : SMT significantly inhibited mucin release from cultured HTSE cells, without cytotoxicity. P-C significantly increased mucin release from cultured HTSE cells, with significant cytotoxicity. SMT inhibited Ach-induced contraction of isolated tracheal smooth muscle. P-C did not affect Ach-induced contraction of isolated tracheal smooth muscle. Conclusion : Results suggest that SMT and P-C have regulating effects on mucin secretion from airway goblet cells. Further investigation is needed, because of the value in finding novel agents to this purpose, and these oriental medical prescription have potential for such a role.
참고문헌 (20)
- "Respiratory tract defense mechanism In textbook of pulmonary disease", pp.2~47, 3rd ed Little Brown and Company
- "Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma", 56, pp.345~53 (1981)
- "Drug actions", pp.410~411 (1995), CRC press Inc
- "金匱要略方論本義", pp.226~227 (2000), 남형문화(주)
- "喘息의 原因에 關한 東西醫學的考察", 4, pp.89~ (2000), 韓醫學硏究所 東義韓醫硏
- "Secretions from primary hamster tracheal surface epithelial cells in culture", pp.299~314 (1989), Opaskar Hincman H
- "Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture", pp.4021~7 (1985)
- "Gel contraction causes mucin release in primary hamster tracheal epithelial cells growing on a collagen gel" (1987)
- "Possible requirement of collagen gel substratum for production of mucinlike glycoproteins by primary rabbit tracheal epithelial cells in culture", 21, pp.617~21 (1985)
- "Continuous multiplication of rabbit tracheal epithelial cells in a defined hormone-supplemented medium", 18, pp.800~12 (1982)
- "Expression of tracheal differentiated function in serum-free hormone-supplemented medium", pp.167~81 (1985)
- "Inhibition of mucin release from airway goblet cells by polycationic peptides", 277(21), pp.811~815 (1999)
- "Specificity in the inhibition of mucin release from airway goblet cells by polycationic peptides", 9(3), pp.218~223 (2001)
- "설치류 기관 mucin유리 억제에서의 폴리양이온성 작용기전" (1997), 서울대학교 대학원
- "Quantitation of radiolabeled mucous glycoproteins secreted by tracheal explants", pp.301~6 (1981)
- "Involvement of a signal transduction mechanism in ATP-induced mucin release from cultured airway goblet cells", 8, pp.121~5 (1993)
- "생쥐의 B 세포에서 IgE의 분비와 Cytokine 생산에 대한 小靑龍湯의 效果", 24(2), pp.249~259 (2003)
- "國譯 石室秘錄", pp.34~36 (2000), 書苑堂
- "Measurement of viability and cytotoxicity In Culture of animal cells" (1994), Willey-Liss Inc
- "Physiologic modulation of bronchial epithelial cell barrier function by polycationic exposure"