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四君子湯이 TNBS로 誘發된 생쥐의 炎症性 腸疾患에 미치는 影響

The effect of Sagunja-tang on TNBS-induced Inflammatory Bowel Disease in Mouse

윤성우|김진성|류봉하|홍상선
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2010.12|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제31권 제4호|pp. 731-751

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참고문헌

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42Sagunja-tangInflammatory Bowel Disease6-trinitrobenzene sulfonic acid(TNBS)
주제분류한의학

초록

Objectives : The present study aimed to find out the effect of Sagunja-tang on the prevention and treatment of inflammatory bowel disease using mice with TNBS-induced inflammatory bowel disease. Methods : Mice with TNBS-induced inflammatory bowel disease were medicated with Sagunja-tang, and the weight changes, colon length, lipid peroxidation, and myeloperoxidase activity were observed. Levels of the inflammatory markers interleukin (IL)-1β and cyclooxygenase-2 (COX-2), its transcription factor activation, phospho-NF-κB (pp65), in the colon by enzyme-linked immunosorbent assay and immunoblot analysis were also measured. Finally, the activation of fecal bacterial enzyme, β-glucuronidase and degradation activation of fecal glycosaminoglycan (GAG) and hyaluronic acid were observed. Results : We found that oral administration of Sagunja-tang inhibited TNBS-induced colon shortening and also inhibited myeloperoxidase activity in the colon of mice as well as IL-1β and COX-2 expression. Sagunja-tang also inhibited TNBS-induced lipid peroxidation and pp65 activation in the colon of mice. In addition, Sagunja-tang inhibited β-glucuronidase activation and fecal hyaluronic acid degradation activation. Conclusions : It is supposed that Sagunja-tang has a potential therapeutic effect on inflammatory bowel disease through the inhibition of both NF-κB activation and lipid peroxidation, and the improvement of intestinal conditions.

Abstract

Objectives : The present study aimed to find out the effect of Sagunja-tang on the prevention and treatment of inflammatory bowel disease using mice with TNBS-induced inflammatory bowel disease. Methods : Mice with TNBS-induced inflammatory bowel disease were medicated with Sagunja-tang, and the weight changes, colon length, lipid peroxidation, and myeloperoxidase activity were observed. Levels of the inflammatory markers interleukin (IL)-1β and cyclooxygenase-2 (COX-2), its transcription factor activation, phospho-NF-κB (pp65), in the colon by enzyme-linked immunosorbent assay and immunoblot analysis were also measured. Finally, the activation of fecal bacterial enzyme, β-glucuronidase and degradation activation of fecal glycosaminoglycan (GAG) and hyaluronic acid were observed. Results : We found that oral administration of Sagunja-tang inhibited TNBS-induced colon shortening and also inhibited myeloperoxidase activity in the colon of mice as well as IL-1β and COX-2 expression. Sagunja-tang also inhibited TNBS-induced lipid peroxidation and pp65 activation in the colon of mice. In addition, Sagunja-tang inhibited β-glucuronidase activation and fecal hyaluronic acid degradation activation. Conclusions : It is supposed that Sagunja-tang has a potential therapeutic effect on inflammatory bowel disease through the inhibition of both NF-κB activation and lipid peroxidation, and the improvement of intestinal conditions.

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