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Effects of Dietary Supplementation of Spirulina and Quercetin on Growth, Innate Immune Responses, Disease Resistance Against Edwardsiella tarda, and Dietary Antioxidant Capacity in the Juvenile Olive Flounder Paralichthys olivaceus

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초록

A 10-week feeding trial was conducted to examine the effects of dietary spirulina and quercetin on growth, innate immunity, disease resistance and dietary antioxidant capacity in the juvenile olive flounder. Triplicate groups of fish (initial body weight, 2.9 ± 0.01 g) were fed one of isonitrogenous (48% crude protein) and isocaloric (17.4 MJ/kg DM) experimental diets containing 0% spirulina (as a control), 3.4% spirulina, or 6.8% spirulina with or without supplementation of 0.5% quercetin (designated as CON, SP3.4, SP6.8, and SP6.8 + Q, respectively) at a rate of 3% body mass twice daily. Higher dietary antioxidant capacity was found with spirulina supplementation, and the highest value (P < 0.05) was obtained with SP6.8 + Q diet. At the end of the feeding trial, no significant effects were observed on growth performance, body composition and disease resistance against Edwardsiella tarda. Lysozyme activity was significantly increased by spirulina supplementation (P < 0.05), and the highest value was observed in the group fed SP6.8 + Q diet. Also, significantly higher respiratory burst activity (P < 0.05) was found in SP3.4 group. According to the results of this study, dietary supplementation of 3.4% spirulina may enhance innate immunity of olive flounder.

Abstract

A 10-week feeding trial was conducted to examine the effects of dietary spirulina and quercetin on growth, innate immunity, disease resistance and dietary antioxidant capacity in the juvenile olive flounder. Triplicate groups of fish (initial body weight, 2.9 ± 0.01 g) were fed one of isonitrogenous (48% crude protein) and isocaloric (17.4 MJ/kg DM) experimental diets containing 0% spirulina (as a control), 3.4% spirulina, or 6.8% spirulina with or without supplementation of 0.5% quercetin (designated as CON, SP3.4, SP6.8, and SP6.8 + Q, respectively) at a rate of 3% body mass twice daily. Higher dietary antioxidant capacity was found with spirulina supplementation, and the highest value (P < 0.05) was obtained with SP6.8 + Q diet. At the end of the feeding trial, no significant effects were observed on growth performance, body composition and disease resistance against Edwardsiella tarda. Lysozyme activity was significantly increased by spirulina supplementation (P < 0.05), and the highest value was observed in the group fed SP6.8 + Q diet. Also, significantly higher respiratory burst activity (P < 0.05) was found in SP3.4 group. According to the results of this study, dietary supplementation of 3.4% spirulina may enhance innate immunity of olive flounder.

참고문헌 (54)

  1. Abdel-Tawwab M, "Live spirulina (Arthrospira platensis) as a growth and immunity promoter for Nile tilapia, Oreochromis niloticus (L.), challenged with pathogenic Aeromonas hydrophila", Aquac Res, 40, pp.1037~1046 (2009)[DOI]
  2. Abdel-Tawwab M, "Compensatory growth, feed utilization, whole-body composition and hematological changes in starved juvenile Nile tilapia, Oreochromis niloticus (L.)", J Appl Aquac, 18, pp.17~36 (2006)[DOI]
  3. Anderson DP, "Basic haematology and serology for fish health programs, In Diseases in Asian Aquaculture II", pp.185~202 (1995), Fish Health Section, Asian Fisheries Society
  4. Andrews SR, "Yeast extract, brewer’s yeast and spirulina in diets for Labeo rohita fingerlings affect haemato-immunological responses and survival following Aeromonas hydrophila challenge", Res Vet Sci, 91, pp.103~109 (2011)[DOI]
  5. Association of Official Analytical Chemists, "Official Methods Analysis. 16th", Association of Official Analytical Chemists, ~ (1995)
  6. Bermejo P, "Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis", Food Chem, 110, pp.436~445 (2008)[DOI]
  7. Bhat VB, "C-phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro", Biochem Biophys Res Commun, 275, pp.20~25 (2000)[DOI]
  8. Brand-Williams W, "Use of a free radical method to evaluate antioxidant activity", LWT Food Sci Technol, 28, pp.25~30 (1995)[DOI]
  9. Brown BA, "Hematology: Principles and Procedures", pp.71~112 (1980), Lea and Febiger
  10. Castaño-Sánchez C, "A second generation genetic linkage map of Japanese flounder (Paralichthys olivaceus)", BMC Genomics, 11, pp.554~ (2010)[DOI]
  11. Cohen Z, "Fatty acid composition of Spirulina and Spirulina-like cyanobacteria in relation to their chemotaxonomy", Phytochemistry, 30, pp.205~206 (1991)[DOI]
  12. El-Sayed AFM, "Evaluation of soybean meal, spirulina meal and chicken offal meal as protein sources for silver seabream (Rhabdosargus sarba) fingerlings", Aquaculture, 127, pp.169~176 (1994)[DOI]
  13. Fauconneau B, "The measurements of whole body protein synthesis in larval and juvenile carp (Cyprinus carpio)", Comp Biochem Physiol B, 78, pp.845~850 (1984)[DOI]
  14. The State of World Fisheries and Aquaculture. Fisheries and Aquaculture Department, "Food and Agriculture Organization of the United Nations", Food and Agriculture Organization of the United Nations, ~ (2010)
  15. Galindo-Villegas J, "Effect of dietary immunostimulants on some innate immune responses and disease resistance against Edwardsiella tarda infection in Japanese flounder (Paralichthys olivaceus)", Suisan Zoshoku, 54, pp.153~162 (2006)
  16. Güroy D, "Effect of dietary Ulva and Spirulina on weight loss and body composition of rainbow trout, Oncorhynchus mykiss (Walbaum), during a starvation period", J Anim Pysiol Anim Nutr (Berl), 95, pp.320~327 (2011)[DOI]
  17. Responsible Use of Antibiotics in Aquaculture, "Hernández Serrano P", FAO, ~, pp.FAO Fisheries Technical Paper. No. 469 (2005)
  18. Kang JH, "Genetic linkage map of olive flounder, Paralichthys olivaceus", Int J Biol Sci, 4, pp.143~149 (2008)
  19. Khan Z, "Nutritional and therapeutic potential of Spirulina", Curr Pharm Biotechnol, 6, pp.373~379 (2005)[DOI]
  20. Lee DJ, "The response of rainbow trout to varying protein", energy ratios in a test diet, 1973, pp.103, 916~922
  21. Lu J, "Ingestion and assimilation of three species of freshwater algae by larval tilapia Oreochromis niloticus", Aquaculture, 238, pp.437~449 (2004)[DOI]
  22. Mahajan G, "γ-Linolenic acid production from Spirulina platensis", Appl Microbiol Biotechnol, 43, pp.466~469 (1995)[DOI]
  23. Matty A, "Evaluation of a yeast, a bacterium and an alga as a protein source for rainbow trout. I. Effect of protein level on growth, gross conversion efficiency and protein conversion efficiency", Aquaculture, 14, pp.235~246 (1978)[DOI]
  24. Meng-Umphan K, "Growth performance, sex hormone levels and maturation ability of Pla Pho (Pangasius bocourti) fed with Spirulina supplementary pellet and hormone application", Int J Agric Biol, 11, pp.458~462 (2009)
  25. Miranda MS, "Antioxidant activity of the microalga Spirulina maxima", Braz J Med Biol Res, 31, pp.1075~1079 (1998)[DOI]
  26. Mustafa MG, "A review: dietary benefits of algae as an additive in fish feed", Isr J Aquac-Bamidgeh, 47, pp.155~162 (1995)
  27. Mustafa MG, "The effect of Spirulina feeding on muscle protein deposition in red sea bream, Pagrus major", J Appl Ichthyol, 10, pp.141~145 (1994)[DOI]
  28. Mustafa MG, "Effects of algae meal as feed additive on growth, feed efficiency, and body composition in red sea bream", Fish Sci, 61, pp.25~28 (1995)
  29. Mustafa MG, "Limited synergistic effect of dietary Spirulina on vitamin C nutrition of red sea bream Pagrus major", J Mar Biotechnol, 5, pp.129~132 (1997)
  30. Nakagawa H, "Algae, In Dietary Supplements for the Health and Quality of Cultured Fish", pp.133~168 (2007), CABI International
  31. Nakazoe J, "Effect of supplementation of alga to the diets on the growth and body composition of nibbler, Girella punctata Grey", Bull Tokai Reg Fish Res Lab, 120, pp.43~51 (1986)
  32. Nandeesha MC, "Effect of feeding Spirulina platensis on the growth, proximate composition and organoleptic quality of common carp, Cyprinus carpio L", Aquac Res, 29, pp.305~312 (1998)[DOI]
  33. Nandeesha MC, "Growth performance of two Indian major carps, catla (Catla catla) and rohu (Labeo rohita) fed diets containing different levels of Spirulina platensis", Bioresour Technol, 80, pp.117~120 (2001)[DOI]
  34. Nieto S, "Flavonoids as stabilizers of fish oil: an alternative to synthetic antioxidants", J Am Oil Chem Soc, 70, pp.773~778 (1993)[DOI]
  35. Ötleş S, "Fatty acid composition of Chlorella and Spirulina microalgae species", J AOAC Int, 84, pp.1708~1714 (2001)
  36. Palmegiano GB, "Spirulina as a nutrient source in diets for growing sturgeon (Acipenser baeri)", Aquac Res, 36, pp.188~195 (2005)[DOI]
  37. Palmegiano GB, "Effects of Spirulina and plant oil on the growth and lipid traits of white sturgeon (Acipenser transmontanus) fingerlings", Aquac Res, 39, pp.587~595 (2008)[DOI]
  38. Peiretti PG, "Effects of diets with increasing levels of Spirulina platensis on the carcass characteristics, meat quality and fatty acid composition of growing rabbits", Livest Sci, 140, pp.218~224 (2011)[DOI]
  39. Promkunthong W, "Results of Spirulina alga on growth and antibody levels in mixed breed Catfish (Clarias macrocephalus × Clarias gariepinus (Burchell)", Songkhlanakarin Technol Sci J, 27, pp.115~132 (2005)
  40. Promya J, "The effects of Spirulina platensis and Cladophora algae on the growth performance, meat quality and immunity stimulating capacity of the African Sharptooth Catfish (Clarias gariepinus)", Int J Agric Biol, 13, pp.77~82 (2011)
  41. Qureshi MA, "Spirulina platensis exposure enhances macrophage phagocytic function in cats", Immunopharmacol Immunotoxicol, 18, pp.457~463 (1996)[DOI]
  42. Reddy CM, "Selective inhibition of cyclooxygenase-2 by C-phycocyanin, a biliprotein from Spirulina platensis", Biochem Biophys Res Commun, 277, pp.599~603 (2000)[DOI]
  43. Rupasinghe HPV, "The role of polyphenols in quality, postharvest handling, and processing of fruits, In Postharvest Biology and Technology of Fruits, Vegetables and Flowers", pp.260~281 (2008), Wiley-Blackwell Publishing
  44. Smith MAK, "Estimation of growth potential by measurement of tissue protein synthetic rates in feeding and fasting rainbow trout, Salmo gairdneri Richardson", J Fish Biol, 19, pp.213~220 (1981)[DOI]
  45. Soivio A, "Fatty acid composition of Coregonus muksun Pallas: changes during incubation, hatching, feeding and starvation", Aquaculture, 79, pp.163~168 (1989)[DOI]
  46. Swain P, "Non-specific immune parameters of brood Indian major carp Labeo rohita and their seasonal variations", Fish Shellfish Immunol, 22, pp.38~43 (2007)[DOI]
  47. Takeuchi T, "Effect on the growth and body composition of juvenile tilapia Oreochromis niloticus fed raw Spirulina", Fish Sci, 68, pp.34~40 (2002)[DOI]
  48. Tongsiri S, "Effect of replacing fishmeal with Spirulina on growth, carcass composition and pigment of the Mekong giant catfish", Asian J Agric Sci, 2, pp.106~110 (2010)
  49. Ungsethaphand T, "Effect of feeding Spirulina platensis on growth and carcass composition of hybrid red tilapia (Oreochromis mossambicus × O. niloticus)", Maejo Int J Sci Technol, 4, pp.331~336 (2010)
  50. Venkataraman LV, "Spirulina platensis (Arthospira): physiology, cell biology and biotechnologym", J Appl Phycol, 9, pp.295~296 (1997)[DOI]
  51. Wang L, "Antioxidant activity of Spirulina platensis extracts by supercritical carbon dioxide extraction", Food Chem, 105, pp.36~41 (2007)[DOI]
  52. Watanabe T, "Effect of dietary Spirulina supplementation on growth performance and flesh lipids of cultured striped jack", J Tokyo Univ Fish, 77, pp.231~239 (1990)
  53. Watanuki H, "Immunostimulant effects of dietary Spirulina platensis on carp, Cyprinus carpio", Aquaculture, 258, pp.157~163 (2006)[DOI]
  54. Zhou J, "Effects of dietary supplementation of A3α-peptidoglycan on innate immune responses and defense activity of Japanese flounder (Paralichthys oilvaceus)", Aquaculture, 251, pp.172~181 (2006)

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