원산지별 홍삼투여에 따른 백서의 자연살해세포 활성도 변화에 대한 연구
Comparative Study of Natural Killer Cell Activity after Red Ginseng Medication on Rat
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Objectives : The purpose of this experiment is comparing the difference on natural killer cell activity through Korean red ginseng and Chinese red ginseng by 51Cr release assay. Methods : Thirty rats were equally divided into a Korean red ginseng group, a Chinese red ginseng group and a control group. Korean and Chinese red ginseng were administrated to the rats at 200mg daily for a weak, while 0.9% normal saline was given to the control. Percent specific lysis (PSL) and lytic units (LU) were calculated from spleen cells by 51Cr release assay. Results : Percent specific lysis of the Korean red ginseng group was significantly higher than that of the control in the ratio of 100:1, effector cell:target cell (p<0.05). Percent specific lysis of Korean red ginseng group was also significantly higher than that of the Chinese red ginseng group in the ratio of 25:1, effector cell:target cell (p<0.05). Chinese red ginseng showed no effect on NK cell activity. Conclusions : These findings suggest that Korean red ginseng improves immunologic function and shows superior effects than Chinese red ginseng.
Abstract
Objectives : The purpose of this experiment is comparing the difference on natural killer cell activity through Korean red ginseng and Chinese red ginseng by 51Cr release assay. Methods : Thirty rats were equally divided into a Korean red ginseng group, a Chinese red ginseng group and a control group. Korean and Chinese red ginseng were administrated to the rats at 200mg daily for a weak, while 0.9% normal saline was given to the control. Percent specific lysis (PSL) and lytic units (LU) were calculated from spleen cells by 51Cr release assay. Results : Percent specific lysis of the Korean red ginseng group was significantly higher than that of the control in the ratio of 100:1, effector cell:target cell (p<0.05). Percent specific lysis of Korean red ginseng group was also significantly higher than that of the Chinese red ginseng group in the ratio of 25:1, effector cell:target cell (p<0.05). Chinese red ginseng showed no effect on NK cell activity. Conclusions : These findings suggest that Korean red ginseng improves immunologic function and shows superior effects than Chinese red ginseng.
참고문헌 (22)
- 이현아, "IL-12 유전자 치료: Systemic immune monitoring", 대한암학회 학술대회, pp.218~ (1999)
- 타다 토미오, "면역의 의미론", pp.189~205 (2000), 도서출판 한울
- Herberman RB, "Natural Killer Cells", Prog Clin Biol Res, 58, pp.33~ (1981)
- Takasugi M, "Reactivity of Lymphocyties from Nomal Person on Cultured Tumor Cells", Cancer Res, 33, pp.2898~ (1973)
- Park, JD., "Recent Studies on the Chemical Cunstituents of Korean ginseng (Panax ginseng C. A. Meyer)", Korean J Ginseng Sci, 20, pp.389~ (1996)
- Kim YS, "Anticancer Activities of Red Ginseng Acidic Polysaccharide by Activation of Macrophages and Natural Killer Cells", 약학회지, 46(2), pp.113~119 (2002)
- Zarling JJ, "Monoclonal antibodies which distinguish between human NK cells and cytotoxic T lymphocytes", Nature, 288, pp.394~396 (1980)
- Herberman RB, "Natural killer cells: Their role in defences against disease", Science, 214, pp.24~30 (1981)
- 대한내과학회, "Harrison's 내과학", pp.2085~2086 (2006), 도서출판MIP
- Henkart MP, "Lymphocyte mediated cytolysis as a secretory phenomenon", Adv Exp Med Biol, 146, pp.227~247 (1982)
- Bradley TP, "Mechamism of cell mediated cytotoxicity at the single cell level", J Immunol, 129, pp.2260~2267 (1982)
- Ulberg M, "Recycling and target binding capacity of human natural killer cells", J Exp Med, 153, pp.615~628 (1981)
- Gidlund M, "cell activity in mice injected with interferon and interferon inducers", Nature, 273, pp.759~768 (1978)
- Omsrud M, "Influence of in vivo hydrocrocortison on some human blood lymphocyte populations", Scand J Immunol, 13, pp.573~577 (1971)
- Friberg DD, "Measurements of Natural Killer (NK) Activity and NK-Cell Quantification", Methods, 9(2), pp.316~326 (1996)
- Motzer SA, "Natural killer cell cytotoxicity: a methods analysis of 51chromium release versus flow cytometry", Biol Res Nurs, 5(2), pp.145~152 (2003)
- Pross HF, "Cytotoxic lymphocytes from normal donors: a functional marker of human non-T lymphocytes", Clin Exp Immunol, 21(2), pp.226~235 (1975)
- 江蘇新醫學院, "中藥大辭典(上卷)", pp.29~32 (1982), 성보출판사
- 지형준, "(대한약전 및 대한약전외)한약규격주해", pp.681~682 (1998), 한국메디칼인덱스사
- 이영철, "보기약류의 면역약리학적 고찰", 대전대학교 한의학연구소 한의학논문집, 9(2), pp.159~171 (2001)
- Kenarova B, "Immunomodulating activity of ginsenoside Rg1 from Panax ginseng", Jpn J Pharmacol, 54(4), pp.447~454 (1990)
- Yun YS, "Effect of red ginseng on natural killer cell activity in mice with lung adenoma induced by urethan and benzo(a)pyrene", Cancer Detect Prev Suppl, 1, pp.301~309 (1987)