The Functional Relevance of Prepro-melanin Concentrating Hormone (pMCH) to Skin Color Change, Blind-side Malpigmentation and Feeding of Oliver Flounder Paralichthys olivaceus
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To assess the functional structure of prepro-melanin-concentrating hormone (pMCH), we isolated and cloned pMCH (of-pMCH) mRNA from the brain of the olive flounder, Paralichthys olivaceus, and compared its amino acid sequence with those from other animals. In addition, to examine whether activation of the brain of-pMCH gene is influenced by background color, density, and feeding, we compared pMCH mRNA activities against different background colors (bright and dark) and at different densities (100% PCA and 200% PCA). To examine whether the pMCH gene is related with malpigmentation of blind-side skin and appetite, we compared pMCH gene expression between ordinary and hypermelanic flounders, and between feeding and fasting flounders. The of-pMCH cDNA was 405 bp in the open reading frame [ORF] and encoded a protein of 135 amino acids; MCH was 51 bp in length and encoded a protein of 17 amino acids. An obvious single band of the expected size was obtained from the brain and pituitary by RT-PCR. In addition, of-pMCH gene activity was significantly higher in the bright background only at low density (< 100% PCA) making the ocular skin of fish whitening, and in ordinary fish. However, the gene activity was significantly decreased in dark background, at high density (>200% PCA), and in hypermelano fish. These results suggest that skin whitening camouflage of the flounder is induced by high MCH gene activity, and the density disturbs the function of background color in the physiological color change. Moreover, our data suggest that a low level of MCH gene activity may be related to malpigmentation of the blind-side skin. In feeding, although pMCH gene activity was significantly increased by feeding in the white background, the pMCH gene activity in the dark background was not influenced by feeding, indicating that the MCH gene activity increased by feeding can be offset by dark background color, or is unaffected by appetite. In conclusion, this study showed that MCH gene expression is related to ocular-skin whitening camouflage and blind-skin hypermelanosis, and is influenced by background color and density.
Abstract
To assess the functional structure of prepro-melanin-concentrating hormone (pMCH), we isolated and cloned pMCH (of-pMCH) mRNA from the brain of the olive flounder, Paralichthys olivaceus, and compared its amino acid sequence with those from other animals. In addition, to examine whether activation of the brain of-pMCH gene is influenced by background color, density, and feeding, we compared pMCH mRNA activities against different background colors (bright and dark) and at different densities (100% PCA and 200% PCA). To examine whether the pMCH gene is related with malpigmentation of blind-side skin and appetite, we compared pMCH gene expression between ordinary and hypermelanic flounders, and between feeding and fasting flounders. The of-pMCH cDNA was 405 bp in the open reading frame [ORF] and encoded a protein of 135 amino acids; MCH was 51 bp in length and encoded a protein of 17 amino acids. An obvious single band of the expected size was obtained from the brain and pituitary by RT-PCR. In addition, of-pMCH gene activity was significantly higher in the bright background only at low density (< 100% PCA) making the ocular skin of fish whitening, and in ordinary fish. However, the gene activity was significantly decreased in dark background, at high density (>200% PCA), and in hypermelano fish. These results suggest that skin whitening camouflage of the flounder is induced by high MCH gene activity, and the density disturbs the function of background color in the physiological color change. Moreover, our data suggest that a low level of MCH gene activity may be related to malpigmentation of the blind-side skin. In feeding, although pMCH gene activity was significantly increased by feeding in the white background, the pMCH gene activity in the dark background was not influenced by feeding, indicating that the MCH gene activity increased by feeding can be offset by dark background color, or is unaffected by appetite. In conclusion, this study showed that MCH gene expression is related to ocular-skin whitening camouflage and blind-skin hypermelanosis, and is influenced by background color and density.
참고문헌 (64)
- Abbott CR, "Identification of hypothalamic nuclei involved in the orexigenic effect of melanin-concentrating hormone", Endocrinology, 144, pp.3943~3949 (2003)
- Amano M, "Melanin-concentrating hormone: A neuropeptide hormone affecting the relationship between photic environment and fish with special reference to background color and food intake regulation", Peptides, 30, pp.1979~1984 (2009)
- Amano M, "Immunocytochemical localization and ontogenic development of melanin-concentrating hormone in the brain of a pleuronectiform fish, the barfin flounder", Cell Tiss Res, 311, pp.71~77 (2003)
- Amiya N, "Effects of tank color on melanin-concentrating hormone levels in the brain, pituitary gland, and plasma of the barfin flounder as revealed by a newly developed time-resolved fluoroimmunoassay", Gen Comp Endocrinol, 143, pp.251~256 (2005)
- Baker B, "Cloning and expression of melanin-concentrating hormone genes in the rainbow trout brain", Neuroendocrinology, 61, pp.67~76 (1995)
- Baker BI, "The role of melanin-concentrating hormone in color change", Annals New York Acad Sci, 680, pp.279~289 (1993)
- Berman JR, "Characterization of two melanin-concentrating hormone genes in zebrafish reveals evolutionary and physiological links with the mammalian MCH system", J Comp Neurol, 517, pp.695~710 (2009)
- Della-Zuana O, "Acute and chronic administration of melanin-concentrating hormone enhances food intake and body weight in Wistar and Sprague-Dawley rats", Internat J Obesity, 26, pp.1289~1295 (2002)
- Doolan BJ, "Effects of cage netting colour and density on the skin pigmentation and stress response of Australian snapper, Pagrus auratus (Bloch & Schneider, 1801)", Aquacult Res, 39, pp.1360~1368 (2008)
- Gomori A, "Chronic intracerebroventricular infusion of MCH causes obesity in mice", Am J Physiol-Endocrinol Metabol, 284, pp.E583~E588 (2003)
- Groneveld D, "Expression of tilapia prepro-melanin-concentrating hormone mRNA in hypothalamic and neurohypophysial cells", J Molec Endocrinol, 14, pp.199~207 (1995)
- Höglund E, "Behavioural and neuroendocrine effects of environmental background colour and social interaction in Arctic charr (Salvelinus alpinus)", J Exper Biol, 205, pp.2535~2543 (2002)
- Han D, "Effect of light intensity on growth, survival and skin color of juvenile Chinese longsnout catfish (Leiocassis longirostris Günther)", Aquaculture, 248, pp.299~306 (2005)
- Iwata N, "Effects of sandy substrate and light on hypermelanosis of the blind side in cultured Japanese flounder Paralichthys olivaceus", Environ Biol Fish, 52, pp.291~297 (1998)
- 정달상, "종묘방류에 따른 넙치, Paralichthys olivaceus 지역집단의 유전학적 구조", 한국어류학회지, 20(3), pp.156~162 (2008), 한국어류학회
- Kalinowski CT, "Effect of different carotenoid sources and their dietary levels on red porgy (Pagrus pagrus) growth and skin colour", Aquaculture, 244, pp.223~231 (2005)
- Kang DY, "Relevance of environmental factors and physiological pigment hormones to blind-side hypermelanosis in the cultured flounder, Paralichthys olivaceus", Aquaculture, 356, pp.14~21 (2012)
- Kang DY, "Functional characterization of two melanin- concentrating hormone genes in the color camouflage, hypermelanosis, and appetite of starry flounder", Gen Comp Endocrinol, 189, pp.74~83 (2013)
- 강덕영, "Effects of Stocking Density on the Blind-side Hypermelanosis of Cultured Olive Flounder Paralichthys olivaceus", Fisheries and Aquatic Sciences, 14(2), pp.123~129 (2011), 한국수산과학회
- Kawauchi H, "Structure and biosynthesis of melanin-concentrating hormone", Life Sci, 45, pp.1133~1140 (1989)
- Kawauchi H, "Functions of melanin-concentrating hormone in fish", J Exper Zool-part A: Comp Exper Biol, 305, pp.751~760 (2006)
- Kawauchi H, "Melanin-concentrating hormone signaling systems in fish", Peptides, 25, pp.1577~1584 (2004)
- Kawauchi H, "Characterization of melanin- concentrating hormone in chum salmon pituitaries", Nature, 305, pp.321~323 (1983)
- Kishida M, "The measurement of melanin-concentrating hormone in trout blood", Gen Comp Endocrinol, 74, pp.221~229 (1989)
- Kobayashi Y, "Transcription elements and functional expression of proopiomelanocortin genes in the pituitary gland of the barfin flounder", Gen Comp Endocrinol, 158, pp.259~267 (2008)
- Lin X, "Brain regulation of feeding behavior and food intake in fish. Comp Biochem Physiol- part A", Molec Integr Physiol, 126, pp.415~434 (2000)
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCT method", Methods, 25, pp.402~408 (2001)
- Ludwig DS, "Melanin-concentrating hormone: A functional melanocortin antagonist in the hypothalamus", Am J Physiol- Endocrinol Metabol, 274, pp.E627~E633 (1998)
- Matsuda K, "Regulation of feeding behavior by pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) in vertebrates", Peptides, 28, pp.1761~1766 (2007)
- Matsuda K, "Regulation of food intake by melanin-concentrating hormone in goldfish", Peptides, 30, pp.2060~2065 (2009)
- Matsuda K, "Central administration of melanin-concentrating hormone (MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus", Neurosci Lett, 399, pp.259~263 (2006)
- Matsuda K, "Feeding-induced changes of melanin-concentrating hormone (MCH)-like immunoreactivity in goldfish brain", Cell Tiss Res, 328, pp.375~382 (2007)
- Minth CD, "Two precursors of melanin-concentrating hormone: DNA sequence analysis and in situ and immunochemical localization", Proc Nat’ Acad Sci US Am, 86, pp.4292~4296 (1989)
- Mizusawa K, "Molecular cloning and expression of two melanin-concentrating hormone receptors in goldfish", Peptides, 30, pp.1990~1996 (2009)
- Nahon JL, "Identification of a single melanin-concentrating hormone messenger ribonucleic acid in coho salmon: Structural relatedness with 7SL ribonucleic acid", J Neuroendocrinol, 3, pp.173~183 (1991)
- Naito N, "Immunocytochemical identification of melanin-concentrating hormone in the brain and pituitary gland of the teleost fishes Oncorhynchus keta and Salmo gairdneri", Cell Tiss Res, 242, pp.41~48 (1985)
- Nery LEM, "Pigment cell signalling for physiological color change. Comp Biochem Physiol-part A", Physiol, 118, pp.1135~1144 (1997)
- Nilsson Sköld H, "Rapid color change in fish and amphibians-function, regulation, and emerging applications", Pigment Cell Melano Res, 26, pp.29~38 (2013)
- Oshima N, "Direct reception of light by chromatophores of lower vertebrates", Pigment Cell Res, 14, pp.312~319 (2001)
- Ottesen OH, "Growth, development, and skin abnormalities of halibut (Hippoglossus hippoglossus L.) juveniles kept on different bottom substrates", Aquaculture, 146, pp.17~25 (1996)
- Pérez Sirkin DI, "Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)", Gen Comp Endocrinol, 176, pp.102~111 (2012)
- Pandolfi M, "Melanin-concentrating hormone system in the brain and skin of the cichlid fish Cichlasoma dimerus: Anatomical localization ontogeny and distribution in comparison to α-melanocyte-stimulating hormone-expressing cells", Cell Tiss Res, 311, pp.61~69 (2003)
- Pissios P, "Expanding the scales: The multiple roles of MCH in regulating energy balance and other biological functions", Endoc Rev, 27, pp.606~620 (2006)
- Presse F, "Melanin-concentrating hormone is a potent anorectic peptide regulated by food-deprivation and glucopenia in the rat", Neuroscience, 71, pp.735~745 (1996)
- Pritchard LE, "Pro-opiomelanocortin processing in the hypothalamus: Impact on melanocortin signalling and obesity", J Endocrinol, 172, pp.411~421 (2002)
- Qu D, "A role for melanin-concentrating hormone in the central regulation of feeding behaviour", Nature, 380, pp.243~247 (1996)
- Ramachandran VS, "Rapid adaptive camouflage in tropical flounders", Nature, 379, pp.815~818 (1996)
- Rossi M, "Melanin-concentrating hormone acutely stimulates feeding, but chronic administration has no effect on body weight", Endocrinology, 138, pp.351~355 (1997)
- Seikai T, "Process of pigment cell differentiation in skin on the left and right side of the Japanese flounder, Paralichthys olivaceus, during metamorphosis", Jap J Ichthyol, 29, pp.85~92 (1992)
- Seikai T, "An association of melanophores appearing at metamorphosis as vehicles of asymmetric skin color formation with pigment anomalies developed under hatchery conditions in the Japanese flounder, Paralichthys olivaceus", Pigment cell research, 1, pp.143~151 (1987)
- Shi Y, "Beyond skin color: Emerging roles of melanin-concentrating hormone in energy homeostasis and other physiological functions", Peptides, 25, pp.1605~1611 (2004)
- Stricker-Krongrad A, "Central and peripheral dysregulation of melanin-concentrating hormone in obese Zucker rats", Mol Brain Res, 92, pp.43~48 (2001)
- Sugimoto M, "Morphological color changes in fish: Regulation of pigment cell density and morphology", Microscopy Res Tech, 58, pp.496~503 (2002)
- Suzuki M, "Influence of environmental colour and diurnal phase on MCH gene expression in the trout", J Neuroendocrinol, 7, pp.319~328 (1995)
- Tagawa M, "Effect of stocking density and addition of proteins on larval survival in Japanese flounder, Paralichthys olivaceus", Aquaculture, 230, pp.517~525 (2004)
- Takahashi A, "The melanin-concentrating hormone receptor 2 (MCH-R2) mediates the effect of MCH to control body color for background adaptation in the barfin flounder", Gen Comp Endocrinol, 151, pp.210~219 (2007)
- Takahashi A, "Possible involvement of melanin-concentrating hormone in food intake in a teleost fish, barfin flounder", Peptides, 25, pp.1613~1622 (2004)
- Takahashi Y, "Influence of stocking density and food at late phase of larval period on hypermelanosis on the blind body side in juvenile Japanese flounder", Nippon Suisan Gakkaishi, 60, pp.593~598 (1994)
- Takayama Y, "Structures of two genes coding for melanin-concentrating hormone of chum salmon", Gene, 80, pp.65~73 (1989)
- Tamura K, "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0", Molecular Biology and Evolution, 24, pp.1596~1599 (2007)
- Tuziak SM, "A preliminary investigation of the role of melanin-concentrating hormone (MCH) and its receptors in appetite regulation of winter flounder (Pseudopleuronectes americanus)", Molec Cell Endocrinol, 348, pp.281~296 (2012)
- Yamanome T, "White background reduces the occurrence of staining, activates melanin-concentrating hormone and promotes somatic growth in barfin flounder", Aquaculture, 244, pp.323~329 (2005)
- Yamanome T, "Melanocyte-stimulating hormone facilitates hypermelanosis on the non-eyed side of the barfin flounder, a pleuronectiform fish", Aquaculture, 270, pp.505~511 (2007)
- Yamanome T, "Hypermelanosis on the blind side of Japanese flounder Paralichthys olivaceus is diminished by rearing in a white tank: Short paper", Fish Sci, 73, pp.466~468 (2007)