Response of body color change rearing under different light intensity conditions in farmed red spotted grouper, Epinephelus akaara
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Background: Fish body color is one of the major factors that determine the commercial value of farmed fish, to understand for coloration mechanisms. The expression of melanin-related genes is according to the developmental stage and light intensity in the red spotted grouper, Epinephelus akaara. Methods: To investigate changes in melanin formation and melanin-related genes in the larval development stage, fish larvae were reared from fertilized egg stage to 50 days after hatching (DAH). Experiment of body color changes was performed under each different light intensity conditions. Melanin-related genes expression was analyzed by real-time qPCR, and body coloration difference was represented by RGB (red, green, blue) code value. Results: Expression levels of melanin-concentrating hormone (MCH) mRNA, pro-opiomelanocortin (POMC) mRNA, and melanocortin 1 receptor (MC1R) mRNA were at their highest 5 DAH (days after hatching). Expression levels of agoutisignaling protein (ASIP) mRNA were at their highest 10 DAH. Results of body color changes according to changes in light intensity conditions showed that the expression level of MCH mRNA in the 2000 lx group was the highest at 6 weeks. The expression levels of POMC mRNA and MC1R mRNA in the 1000 lx group were at their lowest at 9 weeks. RGB color code value under different light intensities were the brightest under 1000 lx and the darkest under 0 lx. Conclusion: Our research suggests that POMC mRNA and MC1R mRNA in the red spotted grouper are involved in melanin synthesis, and these genes are thought to be controlled by light intensity. To better understand the molecular mechanism of coloration in the red spotted grouper, further studies are needed to clarify the relationship between melanophore development and melanin-related genes.
Abstract
Background: Fish body color is one of the major factors that determine the commercial value of farmed fish, to understand for coloration mechanisms. The expression of melanin-related genes is according to the developmental stage and light intensity in the red spotted grouper, Epinephelus akaara. Methods: To investigate changes in melanin formation and melanin-related genes in the larval development stage, fish larvae were reared from fertilized egg stage to 50 days after hatching (DAH). Experiment of body color changes was performed under each different light intensity conditions. Melanin-related genes expression was analyzed by real-time qPCR, and body coloration difference was represented by RGB (red, green, blue) code value. Results: Expression levels of melanin-concentrating hormone (MCH) mRNA, pro-opiomelanocortin (POMC) mRNA, and melanocortin 1 receptor (MC1R) mRNA were at their highest 5 DAH (days after hatching). Expression levels of agoutisignaling protein (ASIP) mRNA were at their highest 10 DAH. Results of body color changes according to changes in light intensity conditions showed that the expression level of MCH mRNA in the 2000 lx group was the highest at 6 weeks. The expression levels of POMC mRNA and MC1R mRNA in the 1000 lx group were at their lowest at 9 weeks. RGB color code value under different light intensities were the brightest under 1000 lx and the darkest under 0 lx. Conclusion: Our research suggests that POMC mRNA and MC1R mRNA in the red spotted grouper are involved in melanin synthesis, and these genes are thought to be controlled by light intensity. To better understand the molecular mechanism of coloration in the red spotted grouper, further studies are needed to clarify the relationship between melanophore development and melanin-related genes.
참고문헌 (62)
- Adachi K, "The histological analysis, colorimetric evaluation, and chemical quantification of melanin content in ‘suntanned’fish", Pigment Cell Res, 18, pp.465~468 (2005)
- Amiya N, "Interaction of orexin", hypocretin-like immunoreactive neurons with melaninconcentrating hormone and α-melanocyte-stimulating hormone neurons in brain of a pleuronectiform fish, barfin flounder, 2008, pp.74, 1040~1046
- Appelbaum S, "Survival, growth, metabolism and behaviour of Clarias gariepinus (Burchell 1822) early stages under different light conditions", Aquacult Eng., 22, pp.269~287 (2000)
- Baker B, "Cloning and expression of melaninconcentrating hormone genes in the rainbow trout brain", Neuroendocrinology, 61, pp.67~76 (1995)
- Batty RS, "Light intensity and the feeding behavior of herring, Clupea harengus", Mar Biol, 107, pp.383~388 (1990)
- Boeuf G, "Does light have an influence on fish growth?", Aquaculture, 177, pp.129~152 (1999)
- Booth MA, "Effects of dietary astaxanthin source and light manipulation on the skin colour of Australian snapper Pagrus auratus (Bloch & Schneider, 1801)", Aquac Res, 35, pp.458~464 (2004)
- Borski RJ, "Fish research and the institutional animal care and use committee", Ilar J, 44, pp.286~294 (2003)
- Cal L, "The International Symposium on Genetics in Aquaculture XII", pp.122~ (2015), Santiago de Compostela
- Cal L, "Fish pigmentation and the melanocortin system", Comp Biochem Phys A, 211, pp.26~33 (2017)
- Cerdá-Reverter JM, "Fish melanocortin system", Eur J Pharmacol, 660, pp.53~60 (2011)
- Cerdá-Reverter JM, "Gene structure of the goldfish agouti-signaling protein : a putative role in the dorsal-ventral pigment pattern of fish", Endocrinology, 146, pp.1597~1610 (2005)
- Chauvet C, "Etude de la croissance du mérou Epinephelus guaza(Linné, 1758)des côtes tunisiennes", Aquat Living Resour, 1, pp.277~288 (1988)
- 조현철, "Effects of Water Temperature Change on the Hematological Responses and Plasma Cortisol Levels in Growing of Red Spotted Grouper, Epinephelus akaara", 발생과 생식, 19(1), pp.19~24 (2015), 한국발생생물학회
- 정인영, "Characterization of Melanin-concentrating Hormone from Olive Flounder (Paralichthys olivaceus)", 생명과학회지, 28(3), pp.284~292 (2018), 한국생명과학회[DOI]
- Cone RD, "Studies on the physiological functions of the melanocortin system", Endocr Rev, 27, pp.736~749 (2006)
- Costin GE, "Human skin pigmentation: melanocytes modulate skin color in response to stress", Faseb J, 21, pp.976~994 (2007)
- Diniz GB, "The melanin-concentrating hormone (MCH) system: a tale of two peptides", Front Neurosci-Switz, 13, pp.1280~ (2019)
- Ducrest AL, "Pleiotropy in the melanocortin system, coloration and behavioural syndromes", Trends Ecol Evol, 23, pp.502~510 (2008)
- Duncan DB, "Multiple range and multiple F tests", Biometrics, 11, pp.1~42 (1955)
- Fujii R, "The physiology of fishes", pp.535~562 (1993), CRC Press
- Fujii R, "The regulation of motile activity in fish chromatophores", Pigm Cell Res, 13, pp.300~319 (2000)
- Fujimoto M, "The background adaptation of the flatfish, Paralichthys olivaceus", Physiol Behav, 50, pp.185~188 (1991)
- Han D, "Effect of light intensity on growth, survival and skin color of juvenile Chinese longsnout catfish (Leiocassis longirostris Günther)", Aquaculture, 248, pp.299~306 (2005)
- Hanlon R, "Cephalopod dynamic camouflage", Curr Biol, 17, pp.400~404 (2007)
- Hur SW, "Characterization of cholecystokinin-producing cells and mucus-secreting goblet cells in the blacktip grouper, Epinephelus fasciatus", Tissue Cell, 45, pp.153~157 (2013)
- Ju SH, "Understanding melanocortin-4 receptor control of neuronal circuits: toward novel therapeutics for obesity syndrome", Pharmacol Res, 129, pp.10~19 (2018)
- Kalinowski CT, "Effect of different carotenoid sources and their dietary levels on red porgy (Pagrus pagrus) growth and skin colour", Aquaculture, 244, pp.223~231 (2005)
- Kasagi S, "The effects of chromatic lights on body color and gene expressions of melanin-concentrating hormone and proopiomelanocortin in goldfish (Carassius auratus)", Gen Comp Endocr, 285, pp.113266~ (2020)
- Kim SH, "Ultrastructure of late spermatids and spermatozoa during spermiogenesis in longtooth grouper Epinephelus bruneus from Jeju, Korea", Tissue Cell, 45, pp.261~268 (2013)
- Kittilsen S, "Melanin-based skin spots reflect stress responsiveness in salmonid fish", Horm Behav, 56, pp.292~298 (2009)
- "Get average color of image" (2018)[URL]
- Kobayashi T, "Tyrosinase related protein 1 (TRP1) functions as a DHICA oxidase in melanin biosynthesis", Embo J, 13, pp.5818~5825 (1994)
- Kohno H, "Morphological development of larval and juvenile grouper, Epinephelus fuscoguttatus", Jpn J Ichthyol, 40, pp.307~316 (1993)
- Kondo T, "Update on the regulation of mammalian melanocyte function and skin pigmentation", Expert Rev Dermatol, 6, pp.97~108 (2011)
- Korner A, "Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin", Science, 217, pp.1163~1165 (1982)
- Lee CH, "Induced maturation and fertilized egg production of the red-spotted grouper, Epinephelus akaara, using adaptive physiology of photoperiod and water temperature", Aquac Res, 00, pp.1~7 (2020)
- 이치훈, "Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone", 발생과 생식, 18(3), pp.127~131 (2014), 한국발생생물학회
- Lee JS, "Effect of light intensity and feed density on feeding behaviour, growth and survival of larval sablefish Anoplopoma fimbria", Aquac Res, 48, pp.4438~4448 (2017)
- Liu Y, "The effect of light intensity on the growth of Brachymystax lenok(Pallas, 1773)", Aquac Res, 43, pp.1838~1844 (2012)
- Luchiari AC, "Light intensity preference of juvenile pikeperch Sander lucioperca (L.)", Aquac Res, 37, pp.1572~1577 (2006)
- Matsuda K, "Recent advances in the regulation of feeding behavior by neuropeptides in fish", Ann Ny Acad Sci, 1163, pp.241~250 (2009)
- Mizusawa K, "Expression of genes for melanotropic peptides and their receptors for morphological color change in goldfish Carassius auratus", Gen Comp Endocr, 264, pp.138~150 (2018)
- Odiorne JM, "The physiology of fishes, vol. 2", pp.387~401 (1957), Academic press
- Paripatananont T, "Effect of astaxanthin on the pigmentation of goldfish Carassius auratus", J World Aquacult Soc, 30, pp.454~460 (1999)
- 박재민, "강담돔 (Oplegnathus punctatus)의 난 발생 및 자치어 형태발달", 한국어류학회지, 27(2), pp.71~77 (2015), 한국어류학회
- Pavlidis M, "Environmental control of skin colour in the red porgy, Pagrus pagrus", Aquac Res, 39, pp.837~849 (2008)
- Petrell RJ, "Effects of pellet contrast and light intensity on salmonid feeding behaviours", Aquacult Eng, 25, pp.175~186 (2001)
- Reichard M, "The effect of light intensity on the drift of young-of-the-year cyprinid fishes", J Fish Biol, 61, pp.1063~1066 (2002)
- Richmond HE, "Light intensity, prey detection and foraging mechanisms of age 0 year yellow perch", J Fish Biol, 65, pp.195~205 (2004)
- Rimmer MA, "A review of grouper (Family Serranidae : Subfamily Epinephelinae) aquaculture from a sustainability science perspective", Rev Aquacult, 11, pp.58~87 (2019)
- Seiji M, "Subcellular localization of melanin biosynthesis", Ann Ny Acad Sci, 100, pp.497~533 (1963)
- Sköld HN, "Rapid color change in fish and amphibians–function, regulation, and emerging applications", Pigm Cell Melanoma R, 26, pp.29~38 (2013)
- Song YB, "Larval development and rearing of longtooth grouper Epinephelus bruneus in Jeju Island, Korea", J World Aquacult Soc, 36, pp.209~216 (2005)
- Sulaimon SS, "The biology of melanocytes", Vet Dermatol, 14, pp.57~65 (2003)
- Suzuki H, "Evolutionary and phylogeographic views on Mc1r and Asip variation in mammals", Genes Genet Syst, 88, pp.155~164 (2013)
- Suzuki I, "Participation of the melanocortin-1 receptor in the UV control of pigmentation", J Invest Derm Sym P., 4, pp.29~34 (1994)
- Takahashi A, "Possible involvement of melanin-concentrating hormone in food intake in a teleost fish, barfin flounder", Peptides, 25, pp.1613~1622 (2004)
- Wang YJ, "Effects of dietary supplementation of carotenoids on survival, growth, pigmentation, and antioxidant capacity of characins, Hyphessobrycon callistus", Aquaculture, 261, pp.641~648 (2006)
- Yamanome T, "White background reduces the occurrence of staining, activates melanin-concentrating hormone and promotes somatic growth in barfin flounder", Aquaculture, 244, pp.323~329 (2005)
- Yamashita YT, "Early growth and development of reciprocal hybrids of the starry flounder Platichthys stellatus and stone flounder Kareius bicoloratus", J Fish Biol, 84, pp.1503~1518 (2014)
- Yasir I, "Effect of light intensity on color performance of false clownfish, Amphiprion ocellaris Cuvier", J World Aquacult Soc, 40, pp.337~350 (2009)
