Evaluation of concurrent vaccinations with recombinant canarypox equine influenza virus and inactivated equine herpesvirus vaccines
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Despite vaccination, equine influenza virus (EIV) and equine herpesvirus (EHV) infections still cause highly contagious respiratory diseases in horses. Recently, concurrent vaccination with EIV and EHV was suggested as a new approach; however, there have been no reports of concurrent vaccination with recombinant canarypox EIV and inactivated EHV vaccines. In this study, we aimed to compare the EIV-specific immune responses induced by concurrent administrations of a recombinant canarypox EIV vaccine and an inactivated bivalent EHV vaccine with those induced by a single recombinant canarypox EIV vaccine in experimental horse and mouse models. Serum and peripheral blood mononuclear cells (PBMCs) were collected from immunized animals after vaccination. EIV-specific serum antibody levels, serum hemagglutinin inhibition (HI) titers, and interferon-gamma (IFN-γ) levels were measured by enzyme-linked immunosorbent assay, HI assay, and quantitative polymerase chain reaction, respectively. Concurrent EIV and EHV vaccine administration significantly increased IFN-γ production, without compromising humoral responses. Our data demonstrate that concurrent vaccination with EIV and EHV vaccines can enhance EIV-specific cellular responses in horses.
Abstract
Despite vaccination, equine influenza virus (EIV) and equine herpesvirus (EHV) infections still cause highly contagious respiratory diseases in horses. Recently, concurrent vaccination with EIV and EHV was suggested as a new approach; however, there have been no reports of concurrent vaccination with recombinant canarypox EIV and inactivated EHV vaccines. In this study, we aimed to compare the EIV-specific immune responses induced by concurrent administrations of a recombinant canarypox EIV vaccine and an inactivated bivalent EHV vaccine with those induced by a single recombinant canarypox EIV vaccine in experimental horse and mouse models. Serum and peripheral blood mononuclear cells (PBMCs) were collected from immunized animals after vaccination. EIV-specific serum antibody levels, serum hemagglutinin inhibition (HI) titers, and interferon-gamma (IFN-γ) levels were measured by enzyme-linked immunosorbent assay, HI assay, and quantitative polymerase chain reaction, respectively. Concurrent EIV and EHV vaccine administration significantly increased IFN-γ production, without compromising humoral responses. Our data demonstrate that concurrent vaccination with EIV and EHV vaccines can enhance EIV-specific cellular responses in horses.
참고문헌 (23)
- Pavulraj S, "Immunogenicity of Calvenza-03 EIV", EHV® vaccine in horses : comparative in vivo study, 2021, pp.9, 166~
- Heldens JGM, "An updated equine influenza vaccine and an equine influenza-herpesvirus combination vaccine containing an immunostim adjuvant provoke equal antibody levels in young foals throughout the primary vaccination course", Vet J, 164, pp.288~291 (2002)[DOI]
- Pavulraj S, "Pathology of equine influenza virus(H3N8)in murine model", PLOS ONE, 10, pp.e0143094~ (2015)[DOI]
- OIE World Organisation for Animal Health, "Equine influenza (infection with equine influenza virus). In: OIE Terrestrial Manual 2019", OIE World Organisation for Animal Health, ~ (2019)
- Paillot R, "A systematic review of recent advances in equine influenza vaccination", Vaccines, 2, pp.797~831 (2014)[DOI]
- Salib FA, "Using indirect ELISA and PCR for the diagnosis of equine herpes virus-1(EHV-1)infection in Egypt", J Vet Med Res, 23, pp.117~124 (2016)[DOI]
- Allkofer A, "Primary vaccination in foals : a comparison of the serological response to equine influenza and equine herpesvirus vaccines administered concurrently or 2 weeks apart", Arch Virol, 166, pp.571~579 (2021)[DOI]
- 조민수, "2016년에서 2018년에 국내 말 인플루엔자 백신 접종 후 항체 양성률", 대한수의학회지, 59(3), pp.151~155 (2019), 대한수의학회
- Paillot R, "Antibody and IFN-γ responses induced by a recombinant canarypox vaccine and challenge infection with equine influenza virus", Vet Immunol Immunopathol, 112, pp.225~233 (2006)[DOI]
- 우인옥, "Antibody responses after vaccination against equine influenza virus in the Republic of Korea in 2019", 예방수의학회지, 44(3), pp.132~135 (2020), 한국예방수의학회[DOI]
- 하종철, "제주지역 말허피스바이러스, 말인플루엔자바이러스 및 선역균에 대한 혈청학적 조사", 한국동물위생학회지, 43(2), pp.59~65 (2020), 한국동물위생학회[DOI]
- Gildea S, "Concurrent vaccination against equine influenza and equine herpesvirus – a practical approach", Influenza Other Respir Viruses, 10, pp.433~437 (2016)[DOI]
- Ohta M, "Antibody responses against equine influenza virus induced by concurrent and by consecutive use of an inactivated equine influenza virus vaccine and a modified live equine herpesvirus type 1 vaccine in thoroughbred racehorses", J Equine Vet Sci, 94, pp.103221~ (2020)[DOI]
- Ko EJ, "Roles of aluminum hydroxide and monophosphoryl lipid A adjuvants in overcoming CD4+ T cell deficiency to induce isotypeswitched IgG antibody responses and protection by T-dependent influenza vaccine", J Immunol, 198, pp.279~291 (2017)[DOI]
- Beekman L, "Comparison of cytokine mRNA expression in the bronchoalveolar lavage fluid of horses with inflammatory airway disease and bronchoalveolar lavage mastocytosis or neutrophilia using REST software analysis", J Vet Intern Med, 26, pp.153~161 (2012)[DOI]
- Kumar R, "Pathological and immunological protection induced by inactivated reverse genetics-based H3N8 equine influenza vaccine candidate in murine model", Acta Virol, 64, pp.359~374 (2020)[DOI]
- Pavulraj S, "Immunogenicity and protective efficacy of inactivated equine influenza(H3N8)virus vaccine in murine model", Vet Microbiol, 210, pp.188~196 (2017)[DOI]
- Paillot R, "Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1", Vaccine, 23, pp.4541~4551 (2005)[DOI]
- Paillot R, "New assays to measure equine influenza virus-specific Type 1 immunity in horses", Vaccine, 25, pp.7385~7398 (2007)[DOI]
- Bryant NA, "Comparison of two modern vaccines and previous influenza infection against challenge with an equine influenza virus from the Australian 2007 outbreak", Vet Res, 41, pp.19~ (2010)[DOI]
- Gildea S, "Humoral response and antiviral cytokine expression following vaccination of thoroughbred weanlings—a blinded comparison of commercially available vaccines", Vaccine, 31, pp.5216~5222 (2013)[DOI]
- Adams AA, "Humoral and cell-mediated immune responses of old horses following recombinant canarypox virus vaccination and subsequent challenge infection", Vet Immunol Immunopathol, 139, pp.128~140 (2011)[DOI]
- Reemers S, "Determining equine influenza virus vaccine efficacy—the specific contribution of strain versus other vaccine attributes", Vaccines, 8, pp.501~ (2020)[DOI]
