The Effects of Different Wavelengths of Light-Emitting Diodes on the Expression of Reproduction-Related Genes in Goldfish Carassius auratus
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
We investigated the differences in the expression of the neurohormones kisspeptin (Kiss) and gonadotropin-inhibitory hormone (GnIH) and cytochrome P450 aromatase (P450arom), gonadotropin hormones (GTHs), and sex steroids in the goldfish Carassius auratus exposed to light-emitting diodes (LEDs). The expression levels of Kiss1, Kiss2, G-protein-coupled receptor 54 (GPR54), GTHs, GnIH, and P450arom were compared between the control (white light) and LED-treated goldfish. Furthermore, we measured the plasma levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The levels of Kiss1 mRNA and protein; Kiss2, GPR54, and GTHα protein; GTH mRNA; and plasma FSH and LH in the hypothalamus and cultured hypothalamus cells were significantly higher in the green and purple LED treatment groups than in the other groups. These results suggested that red LEDs inhibit the sex maturation hormones, Kiss, GPR54, GTHs, and P450arom, and that GnIH plays a role in the negative regulation of reproductive function in goldfish.
Abstract
We investigated the differences in the expression of the neurohormones kisspeptin (Kiss) and gonadotropin-inhibitory hormone (GnIH) and cytochrome P450 aromatase (P450arom), gonadotropin hormones (GTHs), and sex steroids in the goldfish Carassius auratus exposed to light-emitting diodes (LEDs). The expression levels of Kiss1, Kiss2, G-protein-coupled receptor 54 (GPR54), GTHs, GnIH, and P450arom were compared between the control (white light) and LED-treated goldfish. Furthermore, we measured the plasma levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The levels of Kiss1 mRNA and protein; Kiss2, GPR54, and GTHα protein; GTH mRNA; and plasma FSH and LH in the hypothalamus and cultured hypothalamus cells were significantly higher in the green and purple LED treatment groups than in the other groups. These results suggested that red LEDs inhibit the sex maturation hormones, Kiss, GPR54, GTHs, and P450arom, and that GnIH plays a role in the negative regulation of reproductive function in goldfish.
참고문헌 (47)
- Amano M, "Novel fish hypothalamic neuropeptides stimulate the release of gonadotrophins and growth hormone from the pituitary of sockeye salmon", J Endocrinol, 188, pp.417~423 (2006)
- Bentley GE, "Gonadotropin-inhibitory peptide in song sparrows (Melospiza melodia)in different reproductive conditions, and in house sparrows (Passer domesticus) relative to chicken-gonadotropin-releasing hormone", J Neuroendocrinol, 15, pp.794~802 (2003)
- Blázquez M, "Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450aromatase in juvenile sea bass(Dicentrarchus labrax)", Mol Cell Endocrinol, 219, pp.83~94 (2004)
- Boeuf G, "Does light have an influence on fish growth?", Aquaculture, 177, pp.129~152 (1999)
- Bromage N, "The environmental regulation of maturation in farmed finfish with special reference to the role of photoperiod and melatonin", Aquaculture, 197, pp.63~98 (2001)
- Ciccone NA, "Gonadotrophin inhibitory hormone depresses gonadotrophin alpha and follicle-stimulating hormone beta subunit expression in the pituitary of the domestic chicken", J Neuroendocrinol, 16, pp.999~1006 (2004)
- Colledge WH, "Kisspeptins and GnRH neuronal signaling", Trends Endocrinol Metab, 20, pp.115~121 (2009)
- de Vlaming VL, "Effects of photoperiod and temperature on gonadal activity in the cyprinid teleost, Notemigonus crysoleucas", Biol Bull, 148, pp.402~415 (1975)
- Falcón J, "Current knowledge on the melatonin system in teleost fish", Gen Comp Endocrinol, 165, pp.469~482 (2010)
- Funes S, "The KiSS-1 receptor GPR54 is essential for the development of the murine reproductive system", Biochem Biophys Res Commun, 312, pp.1357~1363 (2003)
- Greytak SR, "Isolation and characterization of two cytochrome P450 aromatase forms in killifish(Fundulus heteroclitus) : Differential expression in fish from polluted and unpolluted environments", Aquat Toxicol, 71, pp.371~389 (2005)
- Iwasa T, "Developmental changes in the mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus", Int J Dev Neurosci, 30, pp.31~37 (2012)
- Kim NN, "Kisspeptin regulates the hypothalamus–pituitary-gonad axis gene expression during sexual maturation in the cinnamon clownfish, Amphiprion melanopus", Comp Biochem Physiol B, 168, pp.19~32 (2014)
- Kobayashi M, "In vivo biological activity of recombinant goldfish gonadotropins produced by baculovirus in silkworm larvae", Aquaculture, 256, pp.433~442 (2006)
- Kojima D, "Differential expression of duplicated VAL-opsin genes in the developing zebrafish", Neurochem, 104, pp.1364~1371 (2008)
- Lee YH, "Regulation of plasma gonadotropin II secretion by sex steroids, aromatase inhibitors, and antiestrogens in the protandrous black porgy, Acanthopagrus schlegeli Bleeker", Comp Biochem Physiol B, 129, pp.399~406 (2001)
- Lee YR, "Molecular evolution of multiple forms of kisspeptins and GPR54 receptors in vertebrates", Endocrinology, 150, pp.2837~2846 (2009)
- Lythgoe JN, "The Ecology of Vision", pp.270~ (1979), Clarendon Press
- Mano H, "Exo-rhodopsin : a novel rhodopsin expressed in the zebrafish pineal gland", Brain Res Mol Brain Res, 73, pp.110~118 (1999)
- McCullough BD, "Assessing the reliability of statistical software : Part II", The American Statistician, 53, pp.149~159 (1999)
- McFarland WN, "The Ecology of Fishes on Coral Reefs", pp.16~38 (1991), Academic Press
- Migaud H, "The effect of spectral composition and light intensity on melatonin, stress and retinal damage in post-smolt Atlantic salmon, Salmo salar", Aquaculture, 270, pp.390~404 (2007)
- Tae Sun Min, "Sex- and Tissue-related Expression of Two Types of P450 Aromatase mRNA in the Protandrous Black Porgy, Acanthopagrus schlegeli, during Sex Reversal: Expression Profiles Following Exogenous Hormone Administration", Animal Cells and Systems, 13(4), pp.439~445 (2009), 한국통합생물학회
- Nelson ER, "Homologous regulation of estrogen receptor subtypes in goldfish(Carassius auratus)", Mol Reprod Dev, 74, pp.1105~1112 (2007)
- Pankhurst NW, "Cold and dark or warm and light : variations on the theme of environmental control of reproduction", Fish Physiol Biochem, 28, pp.385~389 (2003)
- Parhar IS, "Laser-captured single digoxigenin-labeled neurons of gonadotropin-releasing hormone types reveal a novel G protein-coupled receptor(Gpr54)during maturation in cichlid fish", Endocrinology, 145, pp.3613~3618 (2004)
- Patil J, "Tissue and sexually dimorphic expression of ovarian and brain aromatase mRNA in the Japanese medaka(Oryzias latipes) : Implications for their preferential roles in ovarian and neural differentiation and development", Gen Comp Endocrinol, 158, pp.131~137 (2008)
- Poling MC, "Development, sex steroid regulation, and phenotypic characterization of RFamide-related peptide(Rfrp)gene expression and RFamide receptors in the mouse hypothalamus", Endocrinology, 153, pp.1827~1840 (2012)
- Roa J, "Kisspeptins in reproductive biology : consensus knowledge and recent developments", Biol Reprod, 85, pp.650~660 (2011)
- Roch GJ, "Evolution of GnRH : diving deeper", Gen Comp Endocrinol, 171, pp.1~16 (2011)
- Sawyer SJ, "Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish : Gene specific tissue distribution, sex differences, developmental programming, and estrogen regulation", Gen Comp Endocrinol, 147, pp.108~117 (2006)
- Seminara SB, "The GPR54 gene as a regulator of puberty", N Engl J Med, 349, pp.1614~1627 (2003)
- Shin HS, "Effects of LED light spectra on oxidative stress and the protective role of melatonin in relation to the daily rhythm of the yellowtail clownfish, Amphiprion clarkia", Comp Biochem Physiol A, 160, pp.221~228 (2011)
- Shin HS, "Effect of LED light spectra on the growth of the yellowtail clownfish Amphiprion clarkii", Fish Sci, 78, pp.549~556 (2012)
- Shin HS, "Light-emitting diode spectral sensitivity relationship with reproductive parameters and ovarian maturation in yellowtail damselfish, Chrysiptera parasema", J Photochem Photobiol B, 127, pp.108~113 (2013)
- Shin HS, "The environmental regulation of maturation in goldfish, Carassius auratus : Effects of various LED light spectra", Comp Biochem Physiol A, 168, pp.17~24 (2014)
- Simpson EK, "Aromatase cytochrome P450, the enzyme responsible for estrogen biosynthesis", Endocr Rev, 15, pp.342~355 (1994)
- Smith JT, "Gonadotropin inhibitory hormone function in mammals", Trends Endocrin Met, 21, pp.255~260 (2010)
- Tena-Sempere M, "Comparative insights of the kisspeptin", kisspeptin receptor system : lessons from non-mammalian vertebrates, 2012, pp.175, 234~243
- Trubiroha A, "Up-regulation of gonadotropin mRNA-expression at the onset of gametogenesis in the roach(Rutilus rutilus) : Evidence for an important role of brain-type aromatase(cyp19a1b)in the pituitary", Gen Comp Endocrinol, 178, pp.529~538 (2012)
- Tsutsui K, "Gonadotropin-inhibitory hormone (GnIH) and its control of central and peripheral reproductive function", Front Neuroendocrinol, 31, pp.284~295 (2010)
- Tsutsui K, "A novel avian hypothalamic peptide inhibiting gonadotropin release", Biochem Biophys Res Commun, 275, pp.661~667 (2000)
- Ubuka T, "Gonadotropin-inhibitory hormone neurons interact directly with gonadotropin-releasing hormone-I and-II neurons in European starling brain", Endocrinology, 149, pp.268~278 (2008)
- Um HN, "Molecular co-evolution of kisspeptins and their receptors from fish to mammals", Ann NY Acad Sci, 1200, pp.67~74 (2010)
- Yang B, "Goldfish kisspeptin : Molecular cloning, tissue distribution of transcript expression, and stimulatory effects on prolactin, growth hormone and luteinizing hormone secretion and gene expression via direct actions at the pituitary level", Gen Comp Endocrinol, 165, pp.60~71 (2010)
- Yokoyama K, "The sites of encephalic photoreception in phosoperiodic induction of the growth of the testes in the white-crowned sparrow, Zonotrichia leucophrys gambelii", Cell Tissue Res, 189, pp.441~467 (1978)
- Zhang Y, "Structural diversity of the gnih", gnih receptor system in teleost: Its unvolvement in early development and the negative control of LH release, 2010, pp.31, 1034~1043
