Effects of Different Light Spectra on the Oocyte Maturation in Grass Puffer Takifugu niphobles
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In order to examine the effects of four different light spectra (white, red, green, and blue) on the oocyte maturation, the change of reproductive parameters, via brain-pituitary-gonad (BPG) axis in grass puffer, were investigated. After exposure four different light spectra for 7 weeks, the abundance of gonadotropin-releasing hormone (GnRH) mRNA which is a type of seabream (sbGnRH) and two different subunit of gonadotropin hormones mRNAs, follicle-stimulating hormone (fshβ) mRNA and luteinizing hormone (lhβ) mRNA, were analyzed in the brain and pituitary. Histological analysis showed that the mature oocyte ratio in the green spectrum was higher than other light spectra-exposed groups. Gonadosomatic index (GSI) and oocyte developmental stage were also investigated in the gonad based on histological observations. GSI value with the presence of yolk stage oocytes was significantly increased in the green spectrum-exposed group when compared to that of the other light-exposed groups (white, red, and blue) (p˂0.05). The abundances of sbGnRH mRNA and fshβ mRNA in the green spectrum-exposed group were also significant higher than those of the other light spectra-exposed groups (p˂0.05). These results indicate that the maturation of oocyte in grass puffer can be accelerated by exposure to the spectrum of green. To better understand the molecular mechanism for the maturation of oocyte in grass puffer, further study examining the relationship between oocyte development and its related genes is required.
Abstract
In order to examine the effects of four different light spectra (white, red, green, and blue) on the oocyte maturation, the change of reproductive parameters, via brain-pituitary-gonad (BPG) axis in grass puffer, were investigated. After exposure four different light spectra for 7 weeks, the abundance of gonadotropin-releasing hormone (GnRH) mRNA which is a type of seabream (sbGnRH) and two different subunit of gonadotropin hormones mRNAs, follicle-stimulating hormone (fshβ) mRNA and luteinizing hormone (lhβ) mRNA, were analyzed in the brain and pituitary. Histological analysis showed that the mature oocyte ratio in the green spectrum was higher than other light spectra-exposed groups. Gonadosomatic index (GSI) and oocyte developmental stage were also investigated in the gonad based on histological observations. GSI value with the presence of yolk stage oocytes was significantly increased in the green spectrum-exposed group when compared to that of the other light-exposed groups (white, red, and blue) (p˂0.05). The abundances of sbGnRH mRNA and fshβ mRNA in the green spectrum-exposed group were also significant higher than those of the other light spectra-exposed groups (p˂0.05). These results indicate that the maturation of oocyte in grass puffer can be accelerated by exposure to the spectrum of green. To better understand the molecular mechanism for the maturation of oocyte in grass puffer, further study examining the relationship between oocyte development and its related genes is required.
참고문헌 (19)
- Adams CE, "Photoperiod and tempera-ture effects on early development and reproductive in-vestment in Atlantic salmon (Salmo salar L.)", Aqua-culture, 79, pp.403~409 (1989)
- Bapary MAJ, "The stimulatory effects of long wavelengths of light on the ovarian development in the tropical damselfish, Chrysiptera cyanea", Aquaculture, 314, pp.188~192 (2011)
- Boeuf G, "Does light have an influence on fish growth?", Aquaculture, 177, pp.129~152 (1999)
- Clarke A, "Scaling of metabolic rate with body mass and temperature in teleost fish", J Anim Ecol, 68, pp.893~905 (1999)
- Head AB, "Effects of lighting spectrum and disturbance level on the growth and stress respons-es of yellow perch Perca flavescens", J World Aquacult Soc, 31, pp.73~80 (2000)
- Jonsson N, "Influence of water flow, water tempera-ture and light on fish migration in rivers", Nord J Freshw Res, 66, pp.20~35 (1991)
- Karakatsouli N, "Effects of light spectrum on growth and stress response of rain-bow trout Oncorhynchus mykiss reared under recircu-lating system conditions", Aquacult Eng, 38, pp.36~42 (2008)
- 김병훈, "Relevance of Light Spectra to Growth of the Rearing Tiger Puffer Takifugu rubripes", 발생과 생식, 20(1), pp.23~29 (2016), 한국발생생물학회
- Kojima D, "Vertebrate ancient-long opsin: A green-sensitive photoreceptive molecule present in zebrafish deep brain and retinal horizontal cells", J Neurosci, 20, pp.2845~2851 (2000)
- Oh SH, "Spawning behavior and early life history of Grass Puffer, Takifugu niphobles (Jordan et Snyder) (Teleostei: Tetraodontidae)", Kor J Ichthyol, 12, pp.236~243 (2000)
- Shin HS, "The environmental regulation of maturation in goldfish, Carassius au-ratus: Effects of various LED light spectra", Comp Bio-chem Physiol A Mol Integr Physiol, 168, pp.17~24 (2014)
- 신현옥, "볼락(Sebastes inermis)치어의 성장에 미치는 LED 광파장의 영향", 수산해양기술연구, 51(2), pp.179~187 (2015), 한국수산해양기술학회[DOI]
- Song JA, "Effects of light-emitting diode spectra on the vertebrate ancient long opsin and gonadotropin hor-mone in the goldfish Carassius auratus", Biochem Bio-phys Res Commu, 476, pp.541~545 (2016)
- Takemura A, "External and internal controls of lunar‐related reproductive rhythms in fishes", J Fish biol, 76, pp.7~26 (2010)
- Taylor JF, "Photoperiod can be used to enhance growth and improve feeding efficiency in farmed rainbow trout, Oncorhynchus mykiss", Aquaculture, 256, pp.216~234 (2006)
- Takeuchi Y, "Effects of different colors of light on melatonin suppression and expression analysis of Aanat1 and melanopsin in the eye of a tropical damselfish", Gen Comp Endocr, 204, pp.158~165 (2014)
- Tielmann M, "The effect of light intensity on performance of larval pike-perch (Sander lucioperca)", Aquacult Eng, 77, pp.61~71 (2017)
- Vera LM, "Differen-tial light intensity and spectral sensitivities of Atlantic salmon, European sea bass and Atlantic cod pineal glands ex vivo", Gen Comp Endocrinol, 165, pp.25~33 (2010)
- Yamahira K, "The role of intertidal egg deposition on survival of the puffer, Takifugu niphobles (Jordan et Snyder), embryos", J Exp Mar Biol Ecol, 198, pp.291~306 (1996)