Elastase 매개성 폐조직 손상에 대한 宣肺定喘湯의 보호효과
Protective Effects of Seonpyejeongcheon-tang on Elastase-Induced Lung Injury in Mice
1
조회
0
다운로드
10
인용
0
참고문헌
키워드
초록
Objectives : This study aimed to evaluate the protective effects of Seonpyejeongcheon-tang (SJT) on elastase-induced lung injury. Materials and Methods : The extract of SJT was treated to A549 cells and an elastase-induced lung injury mouse model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed. Results : SJT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, protein level of cyclin B 1, Cdk1, and Erk1/2, and gene expression of TNF-α and IL-1β in A549 cells. SJT treatment also revealed a protective effect on elastase-induced lung injury in mouse model. This effect was evidenced via histopathological findings, including immunofluoresence stains against elastin, collagen, and caspase 3, and protein levels of cyclin B1, Cdc2, and Erk1/2 in lung tissue. Conclusion : These data suggest that SJT has pharmaceutical properties on lung injury. This study thus provides scientific evidence for the efficacy of SJT for clinical application to patients with chronic obstructive pulmonary disease.
Abstract
Objectives : This study aimed to evaluate the protective effects of Seonpyejeongcheon-tang (SJT) on elastase-induced lung injury. Materials and Methods : The extract of SJT was treated to A549 cells and an elastase-induced lung injury mouse model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed. Results : SJT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, protein level of cyclin B 1, Cdk1, and Erk1/2, and gene expression of TNF-α and IL-1β in A549 cells. SJT treatment also revealed a protective effect on elastase-induced lung injury in mouse model. This effect was evidenced via histopathological findings, including immunofluoresence stains against elastin, collagen, and caspase 3, and protein levels of cyclin B1, Cdc2, and Erk1/2 in lung tissue. Conclusion : These data suggest that SJT has pharmaceutical properties on lung injury. This study thus provides scientific evidence for the efficacy of SJT for clinical application to patients with chronic obstructive pulmonary disease.
참고문헌 (39)
- Kim DS, "Prevalence of chronic obstructive pulmonary disease in Korea: a population-based spirometry survey", Am J Respir Crit Care Med., 172(7), pp.842~847 (2005)
- "Korea National Statistical Office/from:"[URL]
- "World Health Organization, Geneva"[URL]
- Lopez AD, "The global burden of disease, 1990-2020", Nat Med, 4(11), pp.1241~1243 (1998)
- 장준, "만성 폐쇄성 폐질환의 약물치료", Tuberculosis and Respiratory Diseases, 59(3), pp.231~241 (2005), 대한결핵및호흡기학회
- 전국한의과대학 폐계내과학교실, "동의폐계내과학", pp.338~346 (2002), 한문화사
- Lee DJ, "The effects of Lonicera japonica on the murine model of Chronic obstructive pulmonary disease", 박사, pp.2009
- 오지석, "加味八味丸의 elastase 유도성 A549 세포사멸에 대한 보호효과", 석사, pp.2009
- 허준, "동의보감", pp.480~ (1986), 남산당
- 전국한의과대학 본초학교실, "本草學", pp.121~ (1994), 영림사
- 정승기, "정천탕이 천식에 미치는 영향에 관한 실험적 연구", 경희의학, 3(1), pp.91~102 (1987)
- 왕중권, "The Effects of Jungchun-tang (定喘湯�) on Respiratory Patterns and Tracheal Tissues in Allergic Asthma", The 10th ICOM 자료집, 102 (1999)
- 김영우, "정천탕과 청상보하탕이 asthma model 내의 cytokine에 미치는 영향", 대한한방내과학회지, 22(3), pp.367~377 (2001)
- 염종훈, "定喘湯과 定喘湯加減方이 알레르기 천식모델 흰쥐의 BALF內 면역세포 및 혈청 IgE에 미치는 영향", 대한한의학회지, 24(1), pp.169~180 (2003), 대한한의학회
- Stoller JK, "Aboussouan LS. Alpha1-antitrypsin deficiency", Lancet, 365(9478), pp.2225~2236 (2005)
- Churg A, "Proteases and emphysema", Curr Opin Pulm Med, 11(2), pp.153~159 (2005)
- Foronjy R, "The role of collagenase in emphysema", Respir Res., 2(6), pp.348~352 (2001)
- 김현국, "만성폐쇄성폐질환의 병태생리", Tuberculosis and Respiratory Diseases, 59(1), pp.1~13 (2005), 대한결핵및호흡기학회
- Hantos Z, "Lung volumes and respiratory mechanics in elastase-induced emphysema in mice", J Appl Physiol., 105(6), pp.1864~1872 (2008)
- Kawakami M, "Sequential and quantitative analysis of a murine model of elastase-induced emphysema", Biol Pharm Bull., 31(7), pp.1434~1438 (2008)
- Nakajoh M, "Retinoic acid inhibits elastase-induced injury in human lung epithelial cell lines", Am J Respir Cell Mol Biol., 28(3), pp.296~304 (2003)
- Lucey EC, "Remodeling of alveolar walls after elastase treatment of hamsters. Results of elastin and collagen mRNA in situ hybridization", Am J Respir Crit Care Med., 158(2), pp.555~564 (1998)
- Pauwels RA, "GOLD Scientific Committee. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. NHLBI", WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary, 2001, pp.163(5), 1256~1276
- Tremblay GM, "Anti-inflammatory activity of neutrophil elastase inhibitors", Curr Opin Investig Drugs, 4(5), pp.556~565 (2003)
- Lungarella G, "The dual role of neutrophil elastase in lung destruction and repair", Int J Biochem Cell Biol., 40, pp.1287~1296 (2008)
- Gardi C, "Testing of compounds in models of pulmonary emphysema", Curr Med Chem., 15(8), pp.803~808 (2008)
- Grütter MG, "Caspases: key players in programmed cell death", Curr Opin Struct Biol, 10(6), pp.649~655 (2000)
- Uhal BD, "Cell cycle kinetics in the alveolar epithelium", Am J Physiol, 272, pp.L1031~L1045 (1997)
- Draetta G, "Cell cycle control in eukaryotes: molecular mechanisms of cdc2 activation", Trends Biochem Sci., 15(10), pp.378~383 (1990)
- Nigg EA, "Vertebrate cdc2 kinase: its regulation by phosphorylation and its mitotic targets", Cold Spring Harb Symp Quant Biol, 56, pp.539~547 (1991)
- Enoch T, "Coupling M phase and S phase: controls maintaining the dependence of mitosis on chromosome replication", Cell, 65(6), pp.921~923 (1991)
- Tsai LH, "Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase", Nature, 353(6340), pp.174~177 (1991)
- Goga A, "Inhibition of CDK1 as a potential therapy for tumors over-expressing MYC", Nat Med, 13(7), pp.820~827 (2007)
- Manes T, "Alpha(v)beta3 integrin expression up-regulates cdc2, which modulates cell migration", J Cell Biol., 161(4), pp.817~826 (2003)
- Segal RA, "Intracellular signaling pathways activated by neurotrophic factors", Annu Rev Neurosci., 19, pp.463~489 (1996)
- Whitmarsh AJ, "Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathways", J Mol Med, 74(10), pp.589~607 (1996)
- Xia Z, "Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis", Science, 270(5240), pp.1326~1331 (1995)
- Ligeza A, "Regulation of ERK1", 2 phosphorylation by acute and chronic morphine - implications for the role of cAMP-responsive element binding factor (CREB) -dependent and Ets-like protein-1 (Elk-1) -dependent transcription; small interfering RNA -based strategy, 2008, pp.275(15), 3836~3849
- Cavanaugh KJ Jr, "Role of stretch on tight junction structure in alveolar epithelial cells", Am J Respir Cell Mol Biol, 25(5), pp.584~591 (2001)