본문 바로가기
KERD
논문KCI 등재

만성폐쇄성폐질환 및 미세먼지 유발 폐손상 동물모델에서 과루행련환의 효과

Effects of Gwaruhaengryeon-hwan on COPD and Particulate Matter Induced Lung Injury on a Mouse Model

박양춘|김승형|양원경|유이란|이철화
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2017.06|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제38권 제3호|pp. 353-366

2

조회

0

다운로드

3

인용

0

참고문헌

키워드

particulate matterchronic obstructive pulmonary diseasecigarette smoke solutionGwaruhaengryeon-hwan
주제분류한의학

초록

Objective: This study aimed to use a mouse model to evaluate the effects of Gwaruhaengryeon-hwan (GHH) on chronic obstructive pulmonary disease (COPD) and particulate matter induced lung injury. Materials and Methods: The study was carried out in two ways (in vitro, in vivo). In vitro RAW 264.7 cells (mouse macrophage) were used and analyzed by flow cytometry, ELISA. In vivo lipopolysaccharide (LPS) and cigarette smoke solution (CSS), or coal, fly ash, diesel exhaust particle (CFD) challenged mice were used and its BALF was analyzed by ELISA, lung tissue by real-time PCR. Results: In vitro, GHH maintained an 80-100% rate of viability. So cytotoxicity was not shown. In the ELISA analysis with RAW 264.7 cells, GHH significantly decreased NO over 30 μg/ml. In the ELISA analysis, GHH significantly decreased TNF-α, IL-6 over 300 μg/ml. In the COPD model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increasing of neutrophils, TNF-α, IL-17A, MIP2, CXCL-1 in BALF, TNF-α, IL-1β mRNA expression in lung tissue and histological lung injury. In the CFD induced lung injury model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increase of neutrophils, TNF-α, IL-17A, MIP2, CXCL-1 in BALF, MUC5AC, TGF-β mRNA expression in lung tissue and histological lung injury. Conclusion: This study suggests the usability of GHH for COPD patients by controlling lung tissue injury.

Abstract

Objective: This study aimed to use a mouse model to evaluate the effects of Gwaruhaengryeon-hwan (GHH) on chronic obstructive pulmonary disease (COPD) and particulate matter induced lung injury. Materials and Methods: The study was carried out in two ways (in vitro, in vivo). In vitro RAW 264.7 cells (mouse macrophage) were used and analyzed by flow cytometry, ELISA. In vivo lipopolysaccharide (LPS) and cigarette smoke solution (CSS), or coal, fly ash, diesel exhaust particle (CFD) challenged mice were used and its BALF was analyzed by ELISA, lung tissue by real-time PCR. Results: In vitro, GHH maintained an 80-100% rate of viability. So cytotoxicity was not shown. In the ELISA analysis with RAW 264.7 cells, GHH significantly decreased NO over 30 μg/ml. In the ELISA analysis, GHH significantly decreased TNF-α, IL-6 over 300 μg/ml. In the COPD model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increasing of neutrophils, TNF-α, IL-17A, MIP2, CXCL-1 in BALF, TNF-α, IL-1β mRNA expression in lung tissue and histological lung injury. In the CFD induced lung injury model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increase of neutrophils, TNF-α, IL-17A, MIP2, CXCL-1 in BALF, MUC5AC, TGF-β mRNA expression in lung tissue and histological lung injury. Conclusion: This study suggests the usability of GHH for COPD patients by controlling lung tissue injury.

참고문헌 (35)

  1. "Global Strategy for Diagnosis, Management, and Prevention of Chronic Obstructive Pulmonary Disease 2017 Report"[URL]
  2. 유철규, "만성폐쇄성폐질환의 병인과 병태생리", 대한내과학회지, 77(4), pp.383~400 (2009), 대한내과학회
  3. Pelkonen M, "Smoking: relationship to chronic bronchitis, chronic obstructive pulmonary disease and mortality", CurrOpinPulm Med, 14(2), pp.105~109 (2008)
  4. Rodriguez-Roisin R, "Toward a consensus definition for COPD exacerbations", Chest, 117, pp.398S~401S (2000)
  5. Sint T, "Ambient air pollution particles and the acute exacerbation of chronic obstructive pulmonary disease", InhalToxicol, 20(1), pp.25~29 (2008)
  6. Ling SH, "Particulate matter air pollution exposure: role in the development and exacerbation of chronic obstructive pulmonary disease", Int J Chron Obstruct Pulmon Dis, 4, pp.233~243 (2009)
  7. World Health Organization, "Air pollution including WHO's 1999 guidelines for air pollution control" (2000), World Health Organization
  8. Pope CA, "Fine-particulate air pollution and life expectancy in the United States", N Engl J Med, 360, pp.376~386 (2009)
  9. 이범준, "만성 폐쇄성 폐질환에 대한 한의학적 변증 평가도구 개발을 위한 기초연구", 대한한의학회지, 33(3), pp.82~94 (2012), 대한한의학회
  10. 이응석, "COPD 동물 모델에서 소청룡탕 흡입제형의 효과", 대한한의학회지, 34(3), pp.54~68 (2013), 대한한의학회
  11. 이주관, "Elastase로 유도된 폐손상에 대한 小靑龍湯의 보호효과", 대한한의학회지, 32(4), pp.83~99 (2011), 대한한의학회
  12. 김형원, "Elastase 매개성 폐조직 손상에 대한 麥門冬湯의 보호효과", 대한한의학회지, 32(2), pp.63~78 (2011), 대한한의학회
  13. 김용, "LPS로 유발된 만성폐쇄성폐질환에 대한 生脈淸肺飮의 영향", 대한한방내과학회지, 32(2), pp.217~231 (2011), 대한한방내과학회
  14. Lee H, "Herbal Formula, PM014, Attenuates Lung Inflammation in a Murine Model of Chronic Obstructive Pulmonary Disease", Evid Based Complement Alternat Med, 2012, pp.769830~ (2012)
  15. 한종민, "만성폐쇄성폐질환 동물모델에 대한 射干湯 및 구성약물의 효과", 대한한의학 방제학회지, 23(2), pp.171~187 (2015), 대한한의학방제학회[DOI]
  16. Hur J, "Dong-Eui-Bo-Gam", pp.473~ (1986), Namsandang
  17. "Revision of the Guidelines for Measures for Hazard component of inhibitor of smoking desire"[URL]
  18. Kim SH, "Method for preparing mouse model induced ambient particulate matter. Korea Patent 10-2016-0156279", ~ (2016)
  19. 정영미, "한국성인의 만성 폐쇄성 폐질환 유병률, 위험요인 및 삶의 질", Journal of Korean Academy of Nursing, 41(2), pp.149~156 (2011), 한국간호과학회
  20. Barnes PJ, "The cytokine network in asthma and chronic obstructive pulmonary disease", J Clin Invest, 118(11), pp.3546~3556 (2008)
  21. Lambrecht BN, "Lung dendritic cells and host immunity to infection", Eur Respir J, 18(4), pp.692~704 (2001)
  22. Wang G, "Genes associated with MUC5AC expression in small airway epithelium of human smokers and nonsmokers", BMC Med Genomics, 5, pp.21~ (2012)
  23. Chen L, "Air particulate pollution and hospital admissions for chronic obstructive pul-monary disease in Reno, Nevada", Inhal Toxicol, 12(4), pp.281~298 (2000)
  24. Sunyer J, "Particles, and not gases, are associated with the risk of death in patients with chronic obstructive pulmonary disease", Int J Epidemiol, 30(5), pp.1138~1140 (2001)
  25. McCreanor J, "Respiratory effects of exposure to diesel traffic in persons with asthma", N Engl J Med, 357(23), pp.2348~2358 (2007)
  26. Gang BS, "Bonchohak", pp.180~ (2006), Younglimsa
  27. Schaberg T, "Superoxide anion release induced by platelet-activating factor is increased in human alveolar macrophages from smokers", Eur Respir J, 5(4), pp.387~393 (1992)
  28. Keatings VM, "Differences in interleukin-8 and tumor necrosis factor-alpha in induced sputum from patients with chronic obstructive pulmonary disease or asthma", Am J Respir Crit Care Med, 153(2), pp.530~534 (1996)
  29. Aaron SD, "Granulocyte inflammatory markers and airway infection during acute exacerbation of chronic obstructive pulmonary disease", Am J Respir Crit Care Med, 163(2), pp.349~355 (2001)
  30. Deveci Y, "Serum ghrelin, IL-6 and TNF-α levels in patients with chronic obstructive pulmonary disease", Tuberk Toraks, 58(2), pp.162~172 (2010)
  31. Levänen B, "Impact of tobacco smoking on cytokine signaling via interleukin-17A in the peripheral airways", Int J Chron Obstruct Pulmon Dis, 11, pp.2109~2116 (2016)
  32. Lukacs NW, "Chemokines and their receptors in chronic pulmonary disease", Curr Drug Targets Inflamm Allergy, 4(3), pp.313~317 (2005)
  33. Traves SL, "Increased levels of the chemokines GROα and MCP-1 in sputum samples from patients with COPD", Thorax, 57(7), pp.590~595 (2002)
  34. Guzman K, "Quantitation of mucin RNA by PCR reveals induction of both MUC2 and MUC5AC mRNA levels by retinoids", Am J Physiol, 271(6 Pt 1), pp.1023~1028 (1996)
  35. Borchers MT, "Regulation of human airway mucins by acrolein and inflammatory mediators", Am J Physiol, 276(4 Pt 1), pp.549~555 (1999)

관련 논문AI추천

표준담배추출물과 Lipopolysaccharide로 유발한 만성폐쇄성폐질환 동물모델에서 쌍화탕의 폐손상 및 근감소 억제 효과김승형, 박양춘, 양원경, 우성천, 이수원, 최진관대한한방내과학회 · 대한한방내과학회지2024 · 제45권 제1호만성폐쇄성폐질환 동물모델에서 SGX01의 폐손상 억제 효과김승형, 박양춘, 박재준, 양원경, 유이란대한한방내과학회 · 대한한방내과학회지2019 · 제40권 제4호미세먼지 in vivo 모델에서 호흡기 질환에 대한 한약의 효과에 관한 연구 동향 분석박양춘, 우성천, 이수원대한한방내과학회 · 대한한방내과학회지2023 · 제44권 제3호LPS로 유발된 만성폐쇄성폐질환에 대한 生脈淸肺飮의 영향김용, 김민희, 남궁욱, 박양춘, 양수영대한한방내과학회 · 대한한방내과학회지2011 · 제32권 제2호Inhibitory Effects of Angelicae Dahuricae Radix Extract on COPD induced by Cigarette Smoke Condensate and Lipopolysaccharide in Mice곽호근, 임흥빈한국약용작물학회 · 한국약용작물학회지2011 · 제19권 제5호蜂毒이 Lipopolisaccharide로 유발된 Chronic Obstructive Pulmonary Disease 病態 Model에 미치는 영향박동희, 정승기, 정희재대한한방내과학회 · 대한한방내과학회지2011 · 제32권 제2호

이 간행물의 다른 논문 (9건)

전립선비대증 변증도구 개발 기초 연구박양춘, 정인철, 조충식, 이지윤, 정호영pp. 301-318한방 치료에 대한 암 환자의 인식 조사 : 단면조사연구윤성수, 이지영, 윤성우, 류한성pp. 319-326고지방식이유도 실험용 Rat에서 Statin 및 한약 2종(후박, 하수오) 투여에 따른 혈중 지질, 조직 변화 비교 연구서윤정, 박성운, 류재환, 최현정pp. 327-335한방의료기관에서의 감기 진료에 대한 일반인의 인식 조사김도형, 조민경, 홍미나, 최준용pp. 336-352茵蔯, 鬱金, 枳實 추출물(ACA)이 HepG2 세포에서 나타나는 이상지질혈증 관련 인자 발현 및 항산화에 미치는 영향조현경, 유호룡, 유주영, 설인찬, 김윤식pp. 367-375
원문 보기