메기(Silurus asotus )에 투여한 멜라민의 체내함량 변화
Accumulation and Depletion of Melamine Through Experimental Feeding in Catfish Silurus asotus
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In 2007, pet food contaminated with melamine caused hundreds of dogs and cats to develop renal failure all over the world. In 2008, over 294,000 infants consumed infant formula and developed kidney stones in China. Further investigation revealed that fish feed also contained melamine; this raised concerns about melamine residues in edible fish tissues, which could have caused the urinary tract stone epidemic. In Korea, catfish fed with assorted feed that included cuttlefish organs that contained melamine developed whitening syndrome and fell dead in some populations in 2008. This event raised suspicions about the toxicity of melamine and all feeds containing melamine were immediately recalled. In this study, we investigated the rates of melamine accumulation and depletion in muscle and viscera of catfish to propose proper withdrawal periods. One group of catfish was fed a commercially available diet that contained 30, 100 and 300 mg melamine per kg diet for 14 days. To investigate residual melamine contents in muscle and viscera, other experimental groups were fed a melamine free diet after being fed melamine for 7 days. The residual amount of melamine was analyzed by LC-MS/MS. The melamine concentration in muscle was estimated to be 3.7mg/kg after 6 days of feeding with a diet containing 300 mg melamine/kg. After 2 days of culture with a melamine free diet, the residual melamine was depleted and the concentration had decreased from 1.15 mg/kg to 0.19 mg/kg in the muscle of catfish fed a diet containing 300 mg melamine/kg for 7 days. The residual amount of melamine was reduced to 0.03 mg/kg in muscle after 7 days of culture with a melamine free diet and was undetectable after a prolonged culture period of 14 days. Catfish tend to excrete melamine rapidly after oral administration and changes in body color were not observed during the short dosing period.
Abstract
In 2007, pet food contaminated with melamine caused hundreds of dogs and cats to develop renal failure all over the world. In 2008, over 294,000 infants consumed infant formula and developed kidney stones in China. Further investigation revealed that fish feed also contained melamine; this raised concerns about melamine residues in edible fish tissues, which could have caused the urinary tract stone epidemic. In Korea, catfish fed with assorted feed that included cuttlefish organs that contained melamine developed whitening syndrome and fell dead in some populations in 2008. This event raised suspicions about the toxicity of melamine and all feeds containing melamine were immediately recalled. In this study, we investigated the rates of melamine accumulation and depletion in muscle and viscera of catfish to propose proper withdrawal periods. One group of catfish was fed a commercially available diet that contained 30, 100 and 300 mg melamine per kg diet for 14 days. To investigate residual melamine contents in muscle and viscera, other experimental groups were fed a melamine free diet after being fed melamine for 7 days. The residual amount of melamine was analyzed by LC-MS/MS. The melamine concentration in muscle was estimated to be 3.7mg/kg after 6 days of feeding with a diet containing 300 mg melamine/kg. After 2 days of culture with a melamine free diet, the residual melamine was depleted and the concentration had decreased from 1.15 mg/kg to 0.19 mg/kg in the muscle of catfish fed a diet containing 300 mg melamine/kg for 7 days. The residual amount of melamine was reduced to 0.03 mg/kg in muscle after 7 days of culture with a melamine free diet and was undetectable after a prolonged culture period of 14 days. Catfish tend to excrete melamine rapidly after oral administration and changes in body color were not observed during the short dosing period.
참고문헌 (18)
- Determination of melamine residures in catfish tissue by triple quadrupole LC-MS-MS with HILIC chromatography In Laboratory Information Bulletin LIB, "Andersen WC", 1~12, pp.No.4396 VoFDAlume 23 (2007)
- Brown CA, "Outbreaks of renal failure associated with melamine and cyanuric acid in dogs and cats in 2004 and 2007", J Vet Diagn Invest, 19, pp.525~531 (2007)
- Cianciolo RE, "Clinicopathologic, histologic, and toxicologic findings in 70 cats inadvertently exposed to pet food contaminated with melamine and cyanuric acid", J Am Vet Med Assoc, 233(5), pp.729~737 (2008)
- Christine MK, "Determination of cyanuric acid residues in catfish, trout, tilapia, salmon and shrimp by liquid chromatography tandem mass spectrometry", Analytica Chimica Acta, 637, pp.101~111 (2009)
- Causon R, "Validation of chromatographic methods in biomedical analysis viewpoint and discussion", J Chromatography B, 689, pp.175~180 (1997)
- Filigenzi MS, "The determination of melamine in muscle tissue by liquid chromatography", tandem mass spectrometry, 2007, pp.21, 4027~4032
- What is the melamine in Food, "KFDA (Korean Food and Drug Administration)", KFDA, 45~50 (2009)
- Lipschitz WL, "The mode of action of three new diuretics: melamine, adenine and formoguanamine", J Pharmacol Exp Ther, 83, pp.234~249 (1945)
- Reimschuessel R, "Evaluation of the renal effects of experimental feeding of melamine and cyanuric acid to fish and pigs", Am J Vet Res, 69, pp.1217~1228 (2008)
- Reimschuessel R, "Residue depletion of melamine and cyanuric acid in catfish and rainbow trout following oral administration", J Vet Pharmacol Therap, 33, pp.172~182 (2009)
- Ronald EB, "Pharmacokinetics of melamine in pigs following intravenous administration", Food Chem Toxicol, 46, pp.1196~1200 (2008)
- Puschner B, "Assessment of Melamine and Cyanuric Acid Toxicity in Cats", J Vet Diagn Invest, 19, pp.616~624 (2007)
- Thompson ME, "Characterization of melamine-containing and calcium oxalate crystals in three dogs with suspected pet foodinduced nephrotoxicosis", Vet Pathol, 45, pp.417~426 (2008)
- Tran BN, "Use of Methanol for the efficient extraction and analysis of melanine and cyanuric acid residues in dairy products and pet foods", J Agric Food Chem, 58, pp.101~107 (2010)
- "Interim melamine and analogues safety/risk assessment"[URL]
- Weil ED, "Flame-retarding plastics and elastomaers with melamine", J Fire Sci, 13, pp.104~126 (1995)
- Wendy CA, "Determination and confirmation of melamine residure in catfis, trout, tilapia, salmon and shrimp by liquid chromatography with tendem mass spectroaetry", J Agric Food Chem, 56, pp.4340~4347 (2008)
- Export meeting to review toxicological aspects of melamine and cyanuric acid. In collaboration with FAO Supported by Health Canada, "WHO", 1~4 (2008)