본문 바로가기
KERD
논문

Intestinal microbial composition changes induced by Lactobacillus plantarum GBL 16, 17 fermented feed and intestinal immune homeostasis regulation in pigs

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

주제분류축산학

초록

In this study, Rubus coreanus (R. coreanus) byproducts with high polyphenol content were fermented with R. coreanus-derived lactic acid bacteria (Lactobacillus plantarum GBL 16 and 17). Then the effect of R. coreanus-derived lactic acid bacteria fermented feed (RC-LAB fermented feed) with probiotics (Bacillus subtills, Aspergillus oryzae, Yeast) as a feed additive for pigs on the composition of intestinal microbes and the regulation of intestinal immune homeostasis was investigated. Seventy-two finishing Berkshire pigs were randomly allotted to four different treatment groups and 18 replicates. RC-LAB fermented feed with probiotics increased the genera Lactobacillus, Streptococcus, Mitsuokella, Prevotella, Bacteroides spp., Roseburia spp., and Faecalibacterium prausnitzii, which are beneficial bacteria of the digestive tract of pigs. Also, RC-LAB fermented feed with probiotics decreased the genera Clostridium, Terrisporobacter, Romboutsia, Kandleria, Megasphaera and Escherichia, which are harmful bacteria. In particular, the relative abundance of the genera Lactobacillus and Streptococcus increased by an average of 8.51% and 4.68% in the treatment groups and the classes Clostridia and genera Escherichia decreased by an average of 27.05% and 2.85% in the treatment groups. In mesenteric lymph nodes (MLN) and spleens, the mRNA expression of transcription factors and cytokines in Th1 and Treg cells increased and the mRNA expression of Th2 and Th17 transcription factors and cytokines decreased, indicating a regulatory effect on intestinal immune homeostasis. RC-LAB fermented feed regulates gut immune homeostasis by influencing the composition of beneficial and detrimental microorganisms in the gut and regulating the balance of Th1/Th2 and Th17/Treg cells.

Abstract

In this study, Rubus coreanus (R. coreanus) byproducts with high polyphenol content were fermented with R. coreanus-derived lactic acid bacteria (Lactobacillus plantarum GBL 16 and 17). Then the effect of R. coreanus-derived lactic acid bacteria fermented feed (RC-LAB fermented feed) with probiotics (Bacillus subtills, Aspergillus oryzae, Yeast) as a feed additive for pigs on the composition of intestinal microbes and the regulation of intestinal immune homeostasis was investigated. Seventy-two finishing Berkshire pigs were randomly allotted to four different treatment groups and 18 replicates. RC-LAB fermented feed with probiotics increased the genera Lactobacillus, Streptococcus, Mitsuokella, Prevotella, Bacteroides spp., Roseburia spp., and Faecalibacterium prausnitzii, which are beneficial bacteria of the digestive tract of pigs. Also, RC-LAB fermented feed with probiotics decreased the genera Clostridium, Terrisporobacter, Romboutsia, Kandleria, Megasphaera and Escherichia, which are harmful bacteria. In particular, the relative abundance of the genera Lactobacillus and Streptococcus increased by an average of 8.51% and 4.68% in the treatment groups and the classes Clostridia and genera Escherichia decreased by an average of 27.05% and 2.85% in the treatment groups. In mesenteric lymph nodes (MLN) and spleens, the mRNA expression of transcription factors and cytokines in Th1 and Treg cells increased and the mRNA expression of Th2 and Th17 transcription factors and cytokines decreased, indicating a regulatory effect on intestinal immune homeostasis. RC-LAB fermented feed regulates gut immune homeostasis by influencing the composition of beneficial and detrimental microorganisms in the gut and regulating the balance of Th1/Th2 and Th17/Treg cells.

참고문헌 (31)

  1. Patil Y, "Interactions between host and gut microbiota in domestic pigs : a review", Gut Microbes, 11, pp.310~334 (2020)[DOI]
  2. Robie Vasquez, "Gut microbiome-produced metabolites in pigs: a review on their biological functions and the influence of probiotics", 한국축산학회지, 64(4), pp.671~695 (2022), 한국축산학회
  3. Bouyahya A, "Chemical compounds of berry-derived polyphenols and their effects on gut microbiota, inflammation, and cancer", Molecules, 27, pp.3286~ (2022)[DOI]
  4. Xue M, "Early and dynamic alterations of Th2", Th1 in previously immunocompetent patients with community-acquired severe sepsis : a prospective observational study, 2019, pp.17, 57~
  5. Zhang S, "The alterations in and the role of the Th17", Treg balance in metabolic diseases, 2021, pp.12, 678355~
  6. Da Yoon Yu, "Effects of Rubus coreanus byproducts on intestinal microbiota and the immune modulation", Animal Bioscience, 31(3), pp.429~438 (2018), 아세아·태평양축산학회[DOI]
  7. De Man JC, "A medium for the cultivation of lactobacilli", J Appl Bacteriol, 23, pp.130~135 (1960)[DOI]
  8. National Academies Press, , 2012
  9. 전윤성, "Improved pipeline for reducing erroneous identification by 16S rRNA sequences using the Illumina MiSeq platform", The Journal of Microbiology, 53(1), pp.60~69 (2015), 한국미생물학회[DOI]
  10. Bunte S, "Intestinal microbiota of fattening pigs offered non-fermented and fermented liquid feed with and without the supplementation of non-fermented coarse cereals", Microorganisms, 8, pp.638~ (2020)[DOI]
  11. 민예진, "Effects of dietary mixture of protease and probiotics on growth performance, blood constituents, and carcass characteristics of growing-finishing pigs", 한국축산학회지, 61(5), pp.272~277 (2019), 한국축산학회[DOI]
  12. Stojanov S, "The influence of probiotics on the firmicutes", bacteroidetes ratio in the treatment of obesity and inflammatory bowel disease, 2020, pp.8, 1715~
  13. Looft T, "In-feed antibiotic effects on the swine intestinal microbiome", Proc Natl Acad Sci USA, 109, pp.1691~1696 (2012)[DOI]
  14. Petrova P, "The complex role of lactic acid bacteria in food detoxification", Nutrients, 14, pp.2038~ (2022)[DOI]
  15. Guo P, "Clostridium species as probiotics : potentials and challenges", J Anim Sci Biotechnol, 11, pp.24~ (2020)[DOI]
  16. Hu J, "Gradual changes of gut microbiota in weaned miniature piglets", Front Microbiol, 7, pp.1727~ (2016)[DOI]
  17. Li N, "Differences in the gut microbiota establishment and metabolome characteristics between low-and normal-birth-weight piglets during early-life", Front Microbiol, 9, pp.1798~ (2018)[DOI]
  18. Zhang D, "Fecal microbiota and its correlation with fatty acids and free amino acids metabolism in piglets after a Lactobacillus strain oral administration", Front Microbiol, 10, pp.785~ (2019)[DOI]
  19. Henneke L, "A dietary carbohydrate – gut Parasutterella – human fatty acid biosynthesis metabolic axis in obesity and type 2 diabetes", Gut Microbes, 14, pp.2057778~ (2022)[DOI]
  20. Amat S, "Prevotella in pigs : the positive and negative associations with production and health", Microorganisms, 8, pp.1584~ (2020)[DOI]
  21. Barka EA, "Taxonomy, physiology, and natural products of actinobacteria", Microbiol Mol Biol Rev, 80, pp.1~43 (2016)[DOI]
  22. Mikkelsen LL, "Enumeration of bifidobacteria in gastrointestinal samples from piglets", Appl Environ Microbiol, 69, pp.654~658 (2003)[DOI]
  23. Presti AL, "Fecal and mucosal microbiota profiling in irritable bowel syndrome and inflammatory bowel disease", Front Microbiol, 10, pp.1655~ (2019)[DOI]
  24. Wu J, "Isolation and genomic characterization of five novel strains of Erysipelotrichaceae from commercial pigs", BMC Microbiol, 21, pp.125~ (2021)[DOI]
  25. Dover LG, "Phylogenomic reappraisal of fatty acid biosynthesis, mycolic acid biosynthesis and clinical relevance among members of the genus Corynebacterium", Front Microbiol, 12, pp.802532~ (2021)[DOI]
  26. Ju Kyoung Oh, "Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota", 한국축산학회지, 63(5), pp.1142~1158 (2021), 한국축산학회[DOI]
  27. Raheem A, "Modulatory effects of probiotics during pathogenic infections with emphasis on immune regulation", Front Immunol, 12, pp.616713~ (2021)[DOI]
  28. Kim HW, "A probiotic mixture regulates T cell balance and reduces atopic dermatitis symptoms in mice", Front Microbiol, 9, pp.2414~ (2018)[DOI]
  29. Kim HW, "Galectin-9 induced by dietary probiotic mixture regulates immune balance to reduce atopic dermatitis symptoms in mice", Front Immunol, 10, pp.3063~ (2020)[DOI]
  30. 김정아, "Anti-Inflammatory Effects of a Mixture of Lactic Acid Bacteria and Sodium Butyrate in Atopic Dermatitis Murine Model", Journal of Medicinal Food, 21(7), pp.716~725 (2018), 한국식품영양과학회[DOI]
  31. Sharma R, "Improvement in Th1", Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice, 2014, pp.36, 9686~

관련 논문AI추천

Effects of lactic acid bacteria fermented feed and three types of lactic acid bacteria (L. plantarum, L. acidophilus, B. animalis) on intestinal microbiota and T cell polarization (Th1, Th2, Th17, Treg) in the intestinal lymph nodes and spleens of rats문양수, 조광근, Jang Jae Cheol, Jung Jong-Hyun, Kim Gwang Il, Kim In Sung, Kim Jeong A, Lee Sang Suk, Oh Sang-Hyon, Park Hwa Chun, Yu Da Yoon아세아·태평양축산학회 · Animal Bioscience2023 · 제36권 제1호Effects of Rubus coreanus byproducts on intestinal microbiota and the immune modulation남기창, 문양수, 박화춘, 이상석, 조광근, Dae Keun Shin, Da Yoon Yu, In Soon Choi, In Sung Kim, Jeong A Kim, Jong Hyun Jung, Sung Hak Kim, Yi Hyung Chung아세아·태평양축산학회 · Animal Bioscience2018 · 제31권 제3호Effects of probiotics on growth performance, intestinal morphology, intestinal microbiota weaning pig challenged with Escherichia coli and Salmonella enterica오한진, 유윤정, Dongcheol Song, Jae Woo An, Ji Hwan Lee, Jin Ho Cho, Kyeongho Jeon, Sehyun Park, Se Yeon Chang, Yohan Yoon, Younghyun Cho한국축산학회 · 한국축산학회지2025 · 제67권 제1호Taking intestinal microbes and the immune system as opportunities to maintain the intestinal health of piglets: reviewChen Fengming, Chen Yue, Hou Xiaohong, Xu Kang, Yin Yulong아세아·태평양축산학회 · Animal Bioscience2025 · 제38권 제9호Influence of host-specific and locally isolated multi-strain probiotics on piglet performance, mortality, inflammatory response, and gut microbiomeArjin Chaiwat, Chaosap Chanporn, Chaweewan Kamon, Hnokaew Patipan, Sahatsanon Katatikarn, Sivapirunthep Panneepa, Sringarm Korawan아세아·태평양축산학회 · Animal Bioscience2025 · 제38권 제4호Effects of Bacillus-based probiotics on growth performance, nutrient digestibility, and intestinal health of weaned pigs류상돈, 백장렬, Daye Mun, Hyunjin Kyoung, Ki Beom Jang, Kyeong Il Park, Min Ho Song, Myunghwan Kong, Younghoon Kim한국축산학회 · 한국축산학회지2021 · 제63권 제6호

이 간행물의 다른 논문 (18건)