Anti-fatigue activity of a mixture of seahorse (Hippocampus abdominalis) hydrolysate and red ginseng
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Seahorse, a syngnathidae fish, is one of the important organisms used in Chinese traditional medicine. Hippocampus abdominalis, a seahorse species successfully cultured in Korea, was validated for use in food by the Ministry of Food and Drug Safety in February 2016; however. the validation was restricted to 50% of the entire composition. Therefore, to use H. abdominalis as a food ingredient, H. abdominalis has to be prepared as a mixture by adding other materials. In this study, the effect of H. abdominalis on muscles was investigated to scientifically verify its potential bioactivity. In addition, the anti-fatigue activity of a mixture comprising H. abdominalis and red ginseng (RG) was evaluated to commercially utilize H. abdominalis in food industry. H. abdominalis was hydrolyzed using Alcalase, a protease, and the effect of H. abdominalis hydrolysate (HH) on the muscles was assessed in C2C12 myoblasts by measuring cell proliferation and glycogen content. In addition, the mixtures comprising HH and RG were prepared at different percentages of RG to HH (20, 30, 40, 50, 60, 70, and 80% RG), and the anti-fatigue activity of these mixtures against oxidative stress was assessed in C2C12 myoblasts. In C2C12 myoblasts, H2O2-induced oxidative stress caused a decrease in viability and physical fatigue-related biomarkers such as glycogen and ATP contents. However, treatment with RG and HH mixtures increased cell viability and the content of fatigue-related biomarkers. In particular, the 80% RG mixture showed an optimum effect on cell viability and ATP synthesis activity. In this study, all results indicated that HH had anti-fatigue activity at concentrations approved for use in food by the law in Korea. Especially, an 80% RG to HH mixture can be used in food for ameliorating fatigue.
Abstract
Seahorse, a syngnathidae fish, is one of the important organisms used in Chinese traditional medicine. Hippocampus abdominalis, a seahorse species successfully cultured in Korea, was validated for use in food by the Ministry of Food and Drug Safety in February 2016; however. the validation was restricted to 50% of the entire composition. Therefore, to use H. abdominalis as a food ingredient, H. abdominalis has to be prepared as a mixture by adding other materials. In this study, the effect of H. abdominalis on muscles was investigated to scientifically verify its potential bioactivity. In addition, the anti-fatigue activity of a mixture comprising H. abdominalis and red ginseng (RG) was evaluated to commercially utilize H. abdominalis in food industry. H. abdominalis was hydrolyzed using Alcalase, a protease, and the effect of H. abdominalis hydrolysate (HH) on the muscles was assessed in C2C12 myoblasts by measuring cell proliferation and glycogen content. In addition, the mixtures comprising HH and RG were prepared at different percentages of RG to HH (20, 30, 40, 50, 60, 70, and 80% RG), and the anti-fatigue activity of these mixtures against oxidative stress was assessed in C2C12 myoblasts. In C2C12 myoblasts, H2O2-induced oxidative stress caused a decrease in viability and physical fatigue-related biomarkers such as glycogen and ATP contents. However, treatment with RG and HH mixtures increased cell viability and the content of fatigue-related biomarkers. In particular, the 80% RG mixture showed an optimum effect on cell viability and ATP synthesis activity. In this study, all results indicated that HH had anti-fatigue activity at concentrations approved for use in food by the law in Korea. Especially, an 80% RG to HH mixture can be used in food for ameliorating fatigue.
참고문헌 (24)
- Deshmukh AS, "Deep proteomics of mouse skeletal muscle enables quantitation of protein isoforms, metabolic pathways, and transcription factors", Mol Cell Proteomics, 14, pp.841~853 (2015)
- Fan H, "Deep sea water improves exercise and inhibits oxidative stress in a physical fatigue mouse model", Biomed Rep, 4, pp.751~757 (2016)
- Huang L-Z, "Bioactivity-guided fractionation for antifatigue property of Acanthopanax senticosus", J Ethnopharmacol, 133, pp.213~219 (2011)
- Huang W-C, "Chicken essence improves exercise performance and ameliorates physical fatigue", Nutrients, 6, pp.2681~2696 (2014)
- Huang W-C, "Effect of curcumin supplementation on physiological fatigue and physical performance in mice", Nutrients, 7, pp.905~921 (2015)
- Jiang D-Q, "Antioxidant and anti-fatigue effects on anthocyanins of mulberry juice purification(MJP)and Mulberry marc purification(MMP)from different varieties mulberry fruit in China", Food Chem Toxicol, 59, pp.1~7 (2013)
- Kang S-M, "Isolation and identification of new compound, 2, 7″-phloroglucinol-6, 6′-bieckol from brown algae, Ecklonia cava and its antioxidant effect", J Funct Foods, 4, pp.158~166 (2012)
- 강나래, "Purification of Antioxidative Peptide from Peptic Hydrolysates of Mideodeok (Styela clava) Flesh Tissue", Food Science and Biotechnology, 22(2), pp.541~547 (2013), 한국식품과학회[DOI]
- Kim H-G, "Antifatigue effects of Panax ginseng CA Meyer: a randomised, double-blind, placebo-controlled trial", PLoS One, 8, pp.e61271~ (2013)
- Kim S, "Anti-fatigue effects of acute red ginseng intake in recovery from repetitive anaerobic exercise", Iran J Public Health, 45, pp.387~389 (2016)
- Kumaravel K, "Antimicrobial effect of five seahorse species from Indian coast", Br J Pharmacol Toxicol, 1, pp.62~66 (2010)
- Kumaravel K, "Seahorses—a source of traditional medicine", Nat Prod Res, 26, pp.2330~2334 (2012)
- Nam S-Y, "Anti-fatigue effect by active dipeptides of fermented porcine placenta through inhibiting the inflammatory and oxidative reactions", Biomed Pharmacother, 84, pp.51~59 (2016)
- Nozawa Y, "The anti-fatigue effects of the low-molecular-weight fraction of bonito extract in mice", Biol Pharm Bull, 32, pp.468~474 (2009)
- Perera N, "Copper-zinc-superoxide dismutase(CuZnSOD), an antioxidant gene from seahorse(Hippocampus abdominalis); molecular cloning, sequence characterization, antioxidant activity and potential peroxidation function of its recombinant protein", Fish Shellfish Immunol, 57, pp.386~399 (2016)
- 천충길, "Isolation and Antioxidant Activity Evaluation of Two New Phthalate Derivatives from Seahorse, Hippocampus Kuda Bleeler", Biotechnology and Bioprocess Engineering, 17(5), pp.1031~1040 (2012), 한국생물공학회
- Segade Á, "Effects of the diet on seahorse(Hippocampus hippocampus)growth, body colour and biochemical composition", Aquacult Nutr, 21, pp.807~813 (2015)
- Singh T, "Mitochondrial dysfunction and chronic fatigue syndromes : issues in clinical care(modified version)", IOSR J Dental Med Sci, 13, pp.30~33 (2014)
- Swamy M, "Anti-fatigue effects of polyphenols extracted from pomegranate peel", Int J Integr Biol, 11, pp.69~72 (2011)
- Tang W, "The anti-fatigue effect of 20(R)-ginsenoside Rg3 in mice by intranasally administration", Biol Pharm Bull, 31, pp.2024~2027 (2008)
- Wu R-E, "Resveratrol protects against physical fatigue and improves exercise performance in mice", Molecules, 18, pp.4689~4702 (2013)
- You L, "In vitro antioxidant activity and in vivo anti-fatigue effect of loach(Misgurnus anguillicaudatus)peptides prepared by papain digestion", Food Chem, 124, pp.188~194 (2011)
- Yu B, "Scavenging and anti-fatigue activity of fermented defatted soybean peptides", Eur Food Res Technol, 226, pp.415~421 (2008)
- Zhang Y, "Anti-fatigue activity of a triterpenoid-rich extract from Chinese bamboo shavings(Caulis bamfusae in taeniam)", Phytother Res, 20, pp.872~876 (2006)