Culturing characteristics of Hanwoo myosatellite cells and C2C12 cells incubated at 37℃ and 39℃ for cultured meat
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To improve culture efficiency of Hanwoo myosatellite cells, these cells were cultured at different temperatures. Hanwoo myosatellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37℃ and 39℃ and determine the possibility of using them as cultured meat. Immunofluorescence staining using Pax7 and Hoechst, both cells cultured at 37℃ proliferated better than cultured at 39℃ (p < 0.05). When differentiated cells were stained with myosin and Hoechst, there was no significant difference in myotube thickness and Fusion index (p > 0.05). In Western blotting analysis, Hanwoo myosatellite cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). C2C12 cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). In reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis, Hanwoo myosatellite cells cultured at 39℃ had significantly (p < 0.05) higher expression levels of MyHC, MYF6, and MB than those cultured at 37℃. C2C12 cells cultured at 39℃ showed significantly (p < 0.05) higher expression levels of MYOG and MB than those cultured at 37℃. To increase culture efficiency of Hanwoo myosatellite cells, proliferating at 37℃ and differentiating at 39℃ are appropriate. Since results of temperature differences of Hanwoo myosatellite cells were similar to those of C2C12 cells, they could be used as a reference for producing cultured meat using Hanwoo satellite cells.
Abstract
To improve culture efficiency of Hanwoo myosatellite cells, these cells were cultured at different temperatures. Hanwoo myosatellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37℃ and 39℃ and determine the possibility of using them as cultured meat. Immunofluorescence staining using Pax7 and Hoechst, both cells cultured at 37℃ proliferated better than cultured at 39℃ (p < 0.05). When differentiated cells were stained with myosin and Hoechst, there was no significant difference in myotube thickness and Fusion index (p > 0.05). In Western blotting analysis, Hanwoo myosatellite cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). C2C12 cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). In reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis, Hanwoo myosatellite cells cultured at 39℃ had significantly (p < 0.05) higher expression levels of MyHC, MYF6, and MB than those cultured at 37℃. C2C12 cells cultured at 39℃ showed significantly (p < 0.05) higher expression levels of MYOG and MB than those cultured at 37℃. To increase culture efficiency of Hanwoo myosatellite cells, proliferating at 37℃ and differentiating at 39℃ are appropriate. Since results of temperature differences of Hanwoo myosatellite cells were similar to those of C2C12 cells, they could be used as a reference for producing cultured meat using Hanwoo satellite cells.
참고문헌 (48)
- Yin H, "Satellite cells and the muscle stem cell niche", Physiol Rev, 93, pp.23~67 (2013)[DOI]
- Post MJ, "Cultured meat from stem cells : challenges and prospects", Meat Sci, 92, pp.297~301 (2012)[DOI]
- 임창현, "저산소 환경의 지구성 운동이 근육 위성세포의 활성 및 증식에 미치는 효과", 운동과학, 28(1), pp.22~30 (2019), 한국운동생리학회
- Kadim IT, "Cultured meat from muscle stem cells : a review of challenges and prospects", J Integr Agric, 14, pp.222~233 (2015)[DOI]
- Choudhury D, "The business of cultured meat", Trends Biotechnol, 38, pp.573~577 (2020)[DOI]
- Faustman C, "Cell-based meat : the need to assess holistically", J Anim Sci, 98, pp.skaa177~ (2020)[DOI]
- Mizuno Y, "Generation of skeletal muscle stem", progenitor cells from murine induced pluripotent stem cells, 2010, pp.24, 2245~2253
- Langelaan MLP, "Advanced maturation by electrical stimulation : differences in response between C2C12and primary muscle progenitor cells", J Tissue Eng Regen Med, 5, pp.529~539 (2011)[DOI]
- Sun SS, "Cloning and culture of muscle satellite cells from Hanwoo and Holstein cattle", Korean J Anim Sci, 42, pp.787~794 (2000)
- 이승환, "Hanwoo cattle: origin, domestication, breeding strategies and genomic selection", 한국축산학회지, 56(1), pp.1~8 (2014), 한국축산학회
- "Imported livestock product inspection results (mid December 2021)" (2021)[URL]
- Bhat ZF, "Prospectus of cultured meat—advancing meat alternatives", J Food Sci Technol, 48, pp.125~140 (2011)[DOI]
- Choi JS, "Research trends in alternative protein and cultured meat materials", Food Ind Nutr, 24, pp.15~20 (2019)
- Edelman PD, "Commentary : in vitro-cultured meat production", Tissue Eng, 11, pp.659~662 (2005)[DOI]
- Simsa R, "Extracellular heme proteins influence bovine myosatellite cell proliferation and the color of cell-based meat", Foods, 8, pp.521~ (2019)[DOI]
- Halevy O, "Early posthatch starvation decreases satellite cell proliferation and skeletal muscle growth in chicks", J Nutr, 130, pp.858~864 (2000)[DOI]
- Yamaguchi T, "Continuous mild heat stress induces differentiation of mammalian myoblasts, shifting fiber type from fast to slow", Am J Physiol Cell Physiol, 298, pp.C140~C148 (2010)[DOI]
- Bolus DJ, "Recurrent heat shock impairs the proliferation and differentiation of C2C12 myoblasts", Cell Stress Chaperones, 23, pp.399~410 (2018)[DOI]
- Sonna LA, "Invited review : effects of heat and cold stress on mammalian gene expression", J Appl Physiol, 92, pp.1725~1742 (2002)[DOI]
- von Maltzahn J, "Pax7 is critical for the normal function of satellite cells in adult skeletal muscle", Proc Natl Acad Sci USA, 110, pp.16474~16479 (2013)[DOI]
- Park HG, "Cellular responses to mild heat stress", Cell Mol Life Sci, 62, pp.10~23 (2005)[DOI]
- Dewey WC, "Failla memorial lecture. The search for critical cellular targets damaged by heat", Radiat Res, 120, pp.191~204 (1989)[DOI]
- Dewey WC, "Arrhenius relationships from the molecule and cell to the clinic", Int J Hyperthermia, 10, pp.457~483 (1994)[DOI]
- van Wijk R, "Increase of epidermal growth factor-stimulated cell-cycle progression and induction of thermotolerance by heat shock : temperature and time relationship", Int J Hyperthermia, 1, pp.147~156 (1985)[DOI]
- Higashikubo R, "Flow cytometric BrdUrd-pulse-chase study of heatinduced cell-cycle progression delays", Cell Prolif, 26, pp.337~348 (1993)[DOI]
- Rowley A, "Heat shock-mediated cell cycle blockage and G1 cyclin expression in the yeast Saccharomyces cerevisiae", Mol Cell Biol, 13, pp.1034~1041 (1993)[DOI]
- Han SI, "Mild heat shock induces cyclin D1 synthesis through multiple Ras signal pathways", FEBS Lett, 515, pp.141~145 (2002)[DOI]
- Han SI, "Implication of a small GTPase Rac1 in the activation of c-Jun N-terminal kinase and heat shock factor in response to heat shock", J Biol Chem, 276, pp.1889~1895 (2001)[DOI]
- Denhardt DT, "Signal-transducing protein phosphorylation cascades mediated by Ras", Rho proteins in the mammalian cell : the potential for multiplex signalling, 1996, pp.318, 729~747
- Kennedy SG, "The PI 3-kinase", Akt signaling pathway delivers an anti-apoptotic signal, 1997, pp.11, 701~713
- Febbraio MA, "Alterations in energy metabolism during exercise and heat stress", Sports Med, 31, pp.47~59 (2001)[DOI]
- Sajjanar B, "Cross-talk between energy metabolism and epigenetics during temperature stress response in C2C12 myoblasts", Int J Hyperthermia, 36, pp.775~783 (2019)[DOI]
- Wang L, "Heat shock protein 70(Hsp70)inhibits oxidative phosphorylation and compensates ATP balance through enhanced glycolytic activity", J Appl Physiol, 113, pp.1669~1676 (2012)[DOI]
- Ellis J, "Proteins as molecular chaperones", Nature, 328, pp.378~379 (1987)[DOI]
- Mishra SR, "Importance of heat shock protein 70 in livestock-at cellular level", J Mol Pathophysiol, 3, pp.30~32 (2014)[DOI]
- Ordway GA, "Myoglobin : an essential hemoprotein in striated muscle", J Exp Biol, 207, pp.3441~3446 (2004)[DOI]
- Kamanga-Sollo E, "Effects of heat stress on proliferation, protein turnover, and abundance of heat shock protein messenger ribonucleic acid in cultured porcine muscle satellite cells", J Anim Sci, 89, pp.3473~3480 (2011)[DOI]
- 김재철, "항 당뇨 약물과 유산소성 트레이닝의 병행 처치가 당뇨병이 유발된 쥐의 골격근내 MyoD, myogenin, mTOR, 및 p-FOXO3 protein의 발현에 미치는 영향", 한국체육학회지, 48(5), pp.507~516 (2009), 한국체육학회
- Enhanced myogenic differentiation of umbilical cord blood stemcells by electric, mechanical, and biochemical multi-signals and development of therapy for muscular diseases, "Kim BS", Ministry of Health and Welfare, ~, pp.Report No.: TRKO201800036973 (2017)
- Kanatous SB, "Regulation of myoglobin expression", J Exp Biol, 213, pp.2741~2747 (2010)[DOI]
- Guo Q, "Controlled heat stress promotes myofibrillogenesis during myogenesis", PLOS ONE, 11, pp.e0166294~ (2016)[DOI]
- Ikeda K, "Effects of heat stimulation and L-ascorbic acid 2-phosphate supplementation on myogenic differentiation of artificial skeletal muscle tissue constructs", J Tissue Eng Regen Med, 11, pp.1322~1331 (2017)[DOI]
- : analysis at the single cell level by digitized imaging microscopy and cell survival, "Cancer Res", 1991, 359~364
- Someren JS, "Heat shock proteins 70 and 90 increase calcineurin activity in vitro through calmodulin-dependent and independent mechanisms", Biochem Biophys Res Commun, 260, pp.619~625 (1999)[DOI]
- Choi YM, "Mild heat stress enhances differentiation and proliferation of Japanese quail myoblasts and enhances slow muscle fiber characteristics", Poult Sci, 95, pp.1912~1917 (2016)[DOI]
- Jampel HD, "Fever and immunoregulation. III. Hyperthermia augments the primary in vitro humoral immune response", J Exp Med, 157, pp.1229~1238 (1983)[DOI]
- Hanson DF, "Fever and the immune response. The effects of physiological temperatures on primary murine splenic T-cell responses in vitro", J Immunol, 151, pp.436~448 (1993)[DOI]
- Hanson DF, "Fever, temperature, and the immune response", Ann N Y Acad Sci, 813, pp.453~464 (1997)[DOI]
