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Characteristics of bovine muscle satellite cell from different breeds for efficient production of cultured meat

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The purpose of this study was comparing in vitro performances of three breeds of donor satellite cells for cultured meat and selecting the optimal donor and providing insight into the selection of donors for cultured meat production. Cattle muscle satellite cells were isolated from the muscle tissue of Hanwoo, Holstein, and Jeju black cattle, and then sorted by fluorescence activated cell sorting (FACS). Regarding proliferation of satellite cells, all three breeds showed similar trends. The myogenic potential, based on PAX7 and MYOD mRNA expression levels, was similar or significantly higher for Holstein than other breeds. When the area, width, and fusion index of the myotube were calculated through immunofluorescence staining of myosin, it was expressed upward for Holstein in all experiments except myotube area at passage 8. In addition, it was confirmed that Holstein’s muscle satellite cells showed an upward expression in the amount of gene and protein expression related to myogenic. In the case of gene expression of MYOG, DES, and MYH4 known to play a key role in differentiation into muscles, it was confirmed that Holstein’s muscle satellite cells expressed higher levels. CAV3, IGF1 and TNNT1, which contribute to hypertrophy and differentiation of muscle cells, showed high expression in Holstein. Our results suggest using cells from Holstein cattle can increase the efficiency of cultured meat production, compared to Hanwoo and Jeju breeds, because the cells exhibit superior differentiation behavior which would lead to greater yields during the maturation phase of bioprocessing.

Abstract

The purpose of this study was comparing in vitro performances of three breeds of donor satellite cells for cultured meat and selecting the optimal donor and providing insight into the selection of donors for cultured meat production. Cattle muscle satellite cells were isolated from the muscle tissue of Hanwoo, Holstein, and Jeju black cattle, and then sorted by fluorescence activated cell sorting (FACS). Regarding proliferation of satellite cells, all three breeds showed similar trends. The myogenic potential, based on PAX7 and MYOD mRNA expression levels, was similar or significantly higher for Holstein than other breeds. When the area, width, and fusion index of the myotube were calculated through immunofluorescence staining of myosin, it was expressed upward for Holstein in all experiments except myotube area at passage 8. In addition, it was confirmed that Holstein’s muscle satellite cells showed an upward expression in the amount of gene and protein expression related to myogenic. In the case of gene expression of MYOG, DES, and MYH4 known to play a key role in differentiation into muscles, it was confirmed that Holstein’s muscle satellite cells expressed higher levels. CAV3, IGF1 and TNNT1, which contribute to hypertrophy and differentiation of muscle cells, showed high expression in Holstein. Our results suggest using cells from Holstein cattle can increase the efficiency of cultured meat production, compared to Hanwoo and Jeju breeds, because the cells exhibit superior differentiation behavior which would lead to greater yields during the maturation phase of bioprocessing.

참고문헌 (38)

  1. Post MJ, "Cultured meat from stem cells : challenges and prospects", Meat Sci, 92, pp.297~301 (2012)[DOI]
  2. Scollan ND, "Future research priorities for animal production in a changing world", Anim Prod Sci, 51, pp.1~5 (2011)[DOI]
  3. Rubio NR, "Plant-based and cell-based approaches to meat production", Nat Commun, 11, pp.6276~ (2020)[DOI]
  4. Post MJ, "Scientific, sustainability and regulatory challenges of cultured meat", Nat Food, 1, pp.403~415 (2020)[DOI]
  5. Mattick CS, "Anticipatory life cycle analysis of in vitro biomass cultivation for cultured meat production in the United States", Environ Sci Technol, 49, pp.11941~11949 (2015)[DOI]
  6. Hopkins PD, "Vegetarian meat : could technology save animals and satisfy meat eaters?", J Agric Environ Ethics, 21, pp.579~596 (2008)[DOI]
  7. Edelman PD, "Commentary : in vitro-cultured meat production", Tissue Eng, 11, pp.659~662 (2005)[DOI]
  8. "Two billion and rising: the global trade in live animals in eight charts" (2020)[URL]
  9. Bredahl L, "Relating consumer perceptions of pork quality to physical product characteristics", Food Qual Prefer, 9, pp.273~281 (1998)[DOI]
  10. Shaikh S, "Cell types used for cultured meat production and the importance of myokines", Foods, 10, pp.2318~ (2021)[DOI]
  11. Chriki S, "Is"cultured meat"a viable alternative to slaughtering animals and a good comprise between animal welfare and human expectations?", Anim Front, 12, pp.35~42 (2022)[DOI]
  12. Melzener L, "Cultured beef : from small biopsy to substantial quantity", J Sci Food Agric, 101, pp.7~14 (2021)[DOI]
  13. "Comparative analysis of cattle breeds as satellite cell donors for cultured beef" (2022)[URL]
  14. Hawke TJ, "Myogenic satellite cells : physiology to molecular biology", J Appl Physiol, 91, pp.534~551 (2001)[DOI]
  15. Jurie C, "Effects of age and breed of beef bulls on growth parameters, carcass and muscle characteristics", Anim Sci, 80, pp.257~263 (2005)[DOI]
  16. Joo ST, "Control of fresh meat quality through manipulation of muscle fiber characteristics", Meat Sci, 95, pp.828~836 (2013)[DOI]
  17. Wegner J, "Growth- and breed-related changes of muscle fiber characteristics in cattle", J Anim Sci, 78, pp.1485~1496 (2000)[DOI]
  18. Olguin HC, "Pax-7 up-regulation inhibits myogenesis and cell cycle progression in satellite cells: a potential mechanism for self-renewal", Dev Biol, 275, pp.375~388 (2004)[DOI]
  19. Zammit PS, "Pax7 and myogenic progression in skeletal muscle satellite cells", J Cell Sci, 119, pp.1824~1832 (2006)[DOI]
  20. Tapscott SJ, "The circuitry of a master switch : myod and the regulation of skeletal muscle gene transcription", Development, 132, pp.2685~2695 (2005)[DOI]
  21. Weintraub H, "The MyoD family and myogenesis : redundancy, networks, and thresholds", Cell, 75, pp.1241~1244 (1993)[DOI]
  22. Sabourin LA, "Reduced differentiation potential of primary MyoD-", -myogenic cells derived from adult skeletal muscle, 1999, pp.144, 631~643
  23. Zhou S, "A long journey before cycling : regulation of quiescence exit in adult muscle satellite cells", Int J Mol Sci, 23, pp.1748~ (2022)[DOI]
  24. Cornforth DP, "Maturity and its relationship to muscle characteristics of cattle", J Anim Sci, 50, pp.75~80 (1980)[DOI]
  25. Hegarty PV, "Comparative growth of porcine skeletal muscles using an indirect prediction of muscle fiber number", Growth, 37, pp.333~344 (1973)
  26. Johnston IA, "Patterns of muscle growth in early and late maturing populations of Atlantic salmon(Salmo salar L. )", Aquaculture, 189, pp.307~333 (2000)[DOI]
  27. Dumont NA, "Satellite cells and skeletal muscle regeneration", Compr Physiol, 5, pp.1027~1059 (2015)[DOI]
  28. Hughes SM, "Selective accumulation of MyoD and myogenin mRNAs in fast and slow adult skeletal muscle is controlled by innervation and hormones", Development, 118, pp.1137~1147 (1993)[DOI]
  29. Hnia K, "Desmin in muscle and associated diseases : beyond the structural function", Cell Tissue Res, 360, pp.591~608 (2015)[DOI]
  30. Ciecierska A, "Transcriptomic profile of primary culture of skeletal muscle cells isolated from semitendinosus muscle of beef and dairy bulls", Int J Mol Sci, 21, pp.4794~ (2020)[DOI]
  31. Kelc R, "Platelet-rich plasma, especially when combined with a TGF-β inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro", PLOS ONE, 10, pp.e0117302~ (2015)[DOI]
  32. Weiss A, "The mammalian myosin heavy chain gene family", Annu Rev Cell Dev Biol, 12, pp.417~439 (1996)[DOI]
  33. Xu Y, "Associations of MYH3 gene copy number variations with transcriptional expression and growth traits in Chinese cattle", Gene, 535, pp.106~111 (2014)[DOI]
  34. Hnasko R, "The biology of caveolae : lessons from caveolin knockout mice and implications for human disease", Mol Interv, 3, pp.445~464 (2003)[DOI]
  35. Quach NL, "Focal adhesion kinase signaling regulates the expression of caveolin 3 and β1 integrin, genes essential for normal myoblast fusion", Mol Biol Cell, 20, pp.3422~3435 (2009)[DOI]
  36. Kumar A, "Muscle-specific expression of insulinlike growth factor 1 improves outcome in Lama2 Dy-w mice, a model for congenital muscular dystrophy type 1A", Hum Mol Genet, 20, pp.2333~2343 (2011)[DOI]
  37. Ascenzi F, "Effects of IGF-1 isoforms on muscle growth and sarcopenia", Aging Cell, 18, pp.e12954~ (2019)[DOI]
  38. Tollefsen SE, "Insulin-like growth factors (IGF) in muscle development: expression of IGF-I, the IGF-I receptor, and an IGF binding protein during myoblast differentiation", J Biol Chem, 264, pp.13810~13817 (1989)[DOI]

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