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金銀花 추출물의 마우스 단회 경구투여 독성실험

Mouse Single Oral Dose Toxicity Test of Lonicerae Flos Aqueous Extracts

최해윤|김종대|박미연|유효정
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2010.09|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제31권 제3호|pp. 539-553

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참고문헌

키워드

Lonicerae FlosToxicity
주제분류한의학

초록

Objectives : The object of this study was to obtain accurate information (single oral dose toxicity) of Lonicerae Flos (LF; Dried flower bud parts of Lonicera japonica Thunb (Caprifoliaceae)), which has traditionally been used in Korean medicine for treating various inflammatory diseases. Methods : In order to observe the 50% lethal dose (LD 50), approximate lethal dosage (ALD) and target organs, test articles were once orally administered to female and male ICR mice at dose levels of 2,000, 1,000, 500 and 0 (control) ㎎/㎏ (body weight.). The mortality and changes on body weight, clinical signs and gross observation were monitored for 14 days after single oral treatment of LF aqueous extracts with organ weights and histopathological observations of 12 types of principle organs. Results :1. After single oral treatment of LF aqueous extracts, we could not find any mortality and toxicological evidences up to 2,000 ㎎/㎏ treated group, the limited dosages in rodents at body and organ weights, clinical signs, gross and histopathological observations. 2. Slight diarrhea was detected in most mice treated with 2,000 ㎎/㎏ of LF aqueous extracts and male mice of LF aqueous extracts 1,000 ㎎/㎏ within 2 days after end of treatment, respectively. Conclusion : The results obtained in this study suggest that the LD 50 and ALD of LF aqueous extracts in both female and male mice after single oral treatment were considered as over 2,000 ㎎/㎏ because no mortalities were detected up to 2000 ㎎/㎏, the highest dose recommended by KFDA and OECD. However, we also observed the possibility of digestive disorders like diarrhea when over 1,000 ㎎/㎏ of LF aqueous extracts were administered in the present study.

Abstract

Objectives : The object of this study was to obtain accurate information (single oral dose toxicity) of Lonicerae Flos (LF; Dried flower bud parts of Lonicera japonica Thunb (Caprifoliaceae)), which has traditionally been used in Korean medicine for treating various inflammatory diseases. Methods : In order to observe the 50% lethal dose (LD 50), approximate lethal dosage (ALD) and target organs, test articles were once orally administered to female and male ICR mice at dose levels of 2,000, 1,000, 500 and 0 (control) ㎎/㎏ (body weight.). The mortality and changes on body weight, clinical signs and gross observation were monitored for 14 days after single oral treatment of LF aqueous extracts with organ weights and histopathological observations of 12 types of principle organs. Results :1. After single oral treatment of LF aqueous extracts, we could not find any mortality and toxicological evidences up to 2,000 ㎎/㎏ treated group, the limited dosages in rodents at body and organ weights, clinical signs, gross and histopathological observations. 2. Slight diarrhea was detected in most mice treated with 2,000 ㎎/㎏ of LF aqueous extracts and male mice of LF aqueous extracts 1,000 ㎎/㎏ within 2 days after end of treatment, respectively. Conclusion : The results obtained in this study suggest that the LD 50 and ALD of LF aqueous extracts in both female and male mice after single oral treatment were considered as over 2,000 ㎎/㎏ because no mortalities were detected up to 2000 ㎎/㎏, the highest dose recommended by KFDA and OECD. However, we also observed the possibility of digestive disorders like diarrhea when over 1,000 ㎎/㎏ of LF aqueous extracts were administered in the present study.

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