청폐사간탕(淸肺瀉肝湯)의 혈소판 응집억제 작용에 대한 in vitro 연구
Anti-platelet Aggregation Effect of Cheongpyesagan-tang In Vitro
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
Objective : The study was designed to test the anti-platelet effect of the extract Cheongpyesagan-tang and compare it with aspirin in vitro. Methods : The extract from Cheongpyesagan-tang was made by the pharmacy department of Kyung Hee Oriental Medical Hospital. The extract was investigated for inhibition against the collagen induced aggregation of human platelet suspensions on aggregometry. Aspirin and aspirin-Cheongpyesagan-tang were investigated together. Results :1. In collagen induced human platelet aggregation test, the extract from Cheongpyesagan-tang significantly inhibited in concentration 30㎎/㎖ (p<0.05), 40㎎/㎖, 50㎎/㎖ (p<0.001) and the effect depended on concentration over 20㎎/㎖. 2. Aspirin and aspirin-Cheongpyesagan-tang inhibited collagen induced human platelet aggregation significantly (p<0.001). Aspirin-extract of Cheongpyesagan-tang inhibition rate was higher than aspirin only (p<0.05). Conclusions : The extract of Cheongpyesagan-tang has anti-platelet aggregation and synergic effect with aspirin on human platelet in vitro.
Abstract
Objective : The study was designed to test the anti-platelet effect of the extract Cheongpyesagan-tang and compare it with aspirin in vitro. Methods : The extract from Cheongpyesagan-tang was made by the pharmacy department of Kyung Hee Oriental Medical Hospital. The extract was investigated for inhibition against the collagen induced aggregation of human platelet suspensions on aggregometry. Aspirin and aspirin-Cheongpyesagan-tang were investigated together. Results :1. In collagen induced human platelet aggregation test, the extract from Cheongpyesagan-tang significantly inhibited in concentration 30㎎/㎖ (p<0.05), 40㎎/㎖, 50㎎/㎖ (p<0.001) and the effect depended on concentration over 20㎎/㎖. 2. Aspirin and aspirin-Cheongpyesagan-tang inhibited collagen induced human platelet aggregation significantly (p<0.001). Aspirin-extract of Cheongpyesagan-tang inhibition rate was higher than aspirin only (p<0.05). Conclusions : The extract of Cheongpyesagan-tang has anti-platelet aggregation and synergic effect with aspirin on human platelet in vitro.
참고문헌 (23)
- Lisa K. Jenings, "Mechanisms of platelet activation: Need for new strategies to protect against platelet -mediated atherothrombosis", Thromb Haemost, 102(2), pp.248~257 (2009)
- Donnan GA, "Stroke", Lancet, 371(9624), pp.1612~1623 (2008)
- ombotic Trialists' Collaboration, "Collaborative meta-analysis of randomised trials of antiplatelet therapy for prevention of death, myocardial infarction, and stroke in high risk patients", BMJ, 324(7329), pp.71~86 (2002)
- Antithrombotic Trialists' (ATT) Collaboration, "Aspirin in the primary and secondary prevention of vascular disease: collaborative meta-analysis of individual participant data from randomised trials", Lancet, 373(9678), pp.1849~1860 (2009)
- Gum PA, "Profile and prevalence of aspirin resistance patients with cardiovascular disease", Am J Cardiol., 88(3), pp.230~235 (2001)
- Holmes DR Jr, "Combining antiplatelet and anticoagulant therapies", J Am Coll Cardiol., 54(2), pp.95~109 (2009)
- 李濟馬, "東醫壽世保元", pp.7~ (1986), 행림출판사
- 김영석, "A comparison Between Stroke Patients Admitted to Oriental Hospital in the years 1987 and 1994", 제9회 국제동양의학학술대회발표논문집, pp.418~455 (1988)
- 권도익, "한방병원 심계내과 입원환자에 대한 임상연구", 경희의학, 12(2), pp.200~213 (1996)
- 장인수, "급성기 뇌졸중 환자에 대한 임상적 고찰", 대한한방내과학회, 21(2), pp.203~212 (2000)
- 최동준, "급성기 중풍 환자에 대한 청폐사간탕의 임상적 효능", 대한한의학회지, 23(4), pp.659~664 (2002), 대한한의학회
- Dong-Hyun Kim, "Antithrombosis of Chungpesagantang and Its Ingredients", Natural Product Sciences, 10(3), pp.109~113 (2004), 한국생약학회
- Born GV, "Inhibition of platelet thrombus formation by chloropromazine acting to diminish haemolysis", Nature, 282, pp.212~213 (1979)
- 洪渟用, "四象診療保元", pp.104~ (1991), 書苑堂
- 尹吉榮, "思象體質醫學論", pp.151~ (1986), 明寶出版社
- 정춘근, "淸肺瀉肝湯이 糖尿誘發 흰쥐의 腦虛血損傷에 미치는 影響", 대한한의학회지, 26(2), pp.217~230 (2005), 대한한의학회
- Eun-KyungPark, "Antithrombotic Activities of Chungpesagantang, Sunghyangjunggisan and Yangkyuksanwhatang", Natural Product Sciences, 8(4), pp.173~176 (2002), 한국생약학회
- Roth GJ, "Aspirin, Platelet, and Thrombosis: Therapy and Practice", Blood, 83(4), pp.885~898 (1994)
- Hankey GJ, "Aspirin resistance", Lancet, 267(9510), pp.606~617 (2006)
- Breddin HK, "Can platelet aggregometry be standardized?", Platelets, 16(3), pp.151~158 (2005)
- Michelson AD, "Current options in platelet function testing", The American journal of cardiology, 98(10A), pp.4N~10N (2006)
- Davi G, "Platelet Activation and atherothrombosis", N ENGL J Med, 357(24), pp.2482~2494 (2007)
- Hu Z, "Herb-drug interactions:a literature review", Drugs, 65(9), pp.1239~1282 (2005)