오적산의 추출 용매 차이에 따른 염증 및 염증 관련 질환에 대한 효능 비교
Effects of water and ethanol extracts from Ojeok-san on inflammation and its related diseases
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Objectives : Ojeok-san, a traditional herbal formula, has been used for the treatment of cold illness and its related symptoms such as headache, nausea and indigestion. This study was performed to compare effects of water (OJSW) and 70% ethanol extracts (OJSE) from Ojeok-san on inflammation and its related diseases atopy, asthma and obesity in vitro. Methods : We performed HPLC to investigate contents of index components of OJSW and OJSE. To investigate the effect of OJSW and OJSE in vitro model, using 5 cell lines, including RAW 264. 7, HaCaT, MC/9, BEAS-2B and 3T3-L1 cell lines. Results : HPLC analysis displayed that the contents of index components were higher in OJSE than OJSW. In lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, OJSE significantly inhibited productions of interleukin (IL)-6, nitrite and prostaglandin E2 (PEG2). In TNF-α/IFN-γ-treated HaCaT eratinocytes, OJSE significantly lowered levels of macrophage-derived chemokine (MDC) as well as regulated and normal T cell expressed and secreted (RANTES). OJSE also had the protective effect on inflammatory response by decreasing RANTES secretion in TNF-α-stimulated BEAS-2B cells. Conclusions : We conclude that OJSE could be more appropriate to enhance the biological activities against inflammation and its related diseases, and could be applied as a bioactive material for developing the potent anti-inflammatory agents.
Abstract
Objectives : Ojeok-san, a traditional herbal formula, has been used for the treatment of cold illness and its related symptoms such as headache, nausea and indigestion. This study was performed to compare effects of water (OJSW) and 70% ethanol extracts (OJSE) from Ojeok-san on inflammation and its related diseases atopy, asthma and obesity in vitro. Methods : We performed HPLC to investigate contents of index components of OJSW and OJSE. To investigate the effect of OJSW and OJSE in vitro model, using 5 cell lines, including RAW 264. 7, HaCaT, MC/9, BEAS-2B and 3T3-L1 cell lines. Results : HPLC analysis displayed that the contents of index components were higher in OJSE than OJSW. In lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, OJSE significantly inhibited productions of interleukin (IL)-6, nitrite and prostaglandin E2 (PEG2). In TNF-α/IFN-γ-treated HaCaT eratinocytes, OJSE significantly lowered levels of macrophage-derived chemokine (MDC) as well as regulated and normal T cell expressed and secreted (RANTES). OJSE also had the protective effect on inflammatory response by decreasing RANTES secretion in TNF-α-stimulated BEAS-2B cells. Conclusions : We conclude that OJSE could be more appropriate to enhance the biological activities against inflammation and its related diseases, and could be applied as a bioactive material for developing the potent anti-inflammatory agents.
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