薏苡仁 추출물이 DSS(dextran sulfate sodium)로 유발된 생쥐의 궤양성 대장염에 미치는 영향
The Effects of Coicis Semen Extract (CSE) on Dextran Sulfate Sodium - Induced Colitis in Mice
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Objectives : This study was carried out to investigate the effects of Coicis Semen Extract (CSE) on the experimental colitis induced by dextran sulfate sodium (DSS) in mice. Methods : Experimental colitis was induced in mice by daily treatment with 5% DSS in the drinking water for 7days in 6-week-old male ICR mice. The colitic mice were divided into three groups: the normal (N) group consisted of mice that were not inflammation-induced. The control (C) group was composed of untreated colitis elicited mice. The sample (S) group was administered CSE after colitis elicitation. The effects on colonic mucosal ulcers were evaluated by the morphological, histological and immunohistochemical change of the large intestine. Results : Inhibition of LPS-induced NO was decreased in S group. Inhibition of LPS-induced iNOS and COX-2 mRNA was noticeably decreased in S group from 0.25mg/ml. In the common morphological and histochemical change, the erosion and the infiltration of inflammatory cells are increased in C group, while they are noticeably decreased in S group. The length of colon was shortened in C group than in S group. The distributions of MUC2 and Hsp70 treated with CSE noticeably increased in S group than in C group (p<0.05). It is confirmed histochemically and immunohistochemically that the distributions of iNOS, COX-2, MAC387, serotonin, apoptosis and PCNA treated with CSE decreased in S group than in C group (p<0.05). Conclusions : It is confirmed that CSE has cytoprotective effect, so can alleviate inflammation process. Therefore, it is expected to have potential protective effect on colitis.
Abstract
Objectives : This study was carried out to investigate the effects of Coicis Semen Extract (CSE) on the experimental colitis induced by dextran sulfate sodium (DSS) in mice. Methods : Experimental colitis was induced in mice by daily treatment with 5% DSS in the drinking water for 7days in 6-week-old male ICR mice. The colitic mice were divided into three groups: the normal (N) group consisted of mice that were not inflammation-induced. The control (C) group was composed of untreated colitis elicited mice. The sample (S) group was administered CSE after colitis elicitation. The effects on colonic mucosal ulcers were evaluated by the morphological, histological and immunohistochemical change of the large intestine. Results : Inhibition of LPS-induced NO was decreased in S group. Inhibition of LPS-induced iNOS and COX-2 mRNA was noticeably decreased in S group from 0.25mg/ml. In the common morphological and histochemical change, the erosion and the infiltration of inflammatory cells are increased in C group, while they are noticeably decreased in S group. The length of colon was shortened in C group than in S group. The distributions of MUC2 and Hsp70 treated with CSE noticeably increased in S group than in C group (p<0.05). It is confirmed histochemically and immunohistochemically that the distributions of iNOS, COX-2, MAC387, serotonin, apoptosis and PCNA treated with CSE decreased in S group than in C group (p<0.05). Conclusions : It is confirmed that CSE has cytoprotective effect, so can alleviate inflammation process. Therefore, it is expected to have potential protective effect on colitis.
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