Establishment of an analytical method for butaphosphan (BTP), a stress-attenuating agent, and its application in the preliminary pharmacokinetic evaluation of residues in olive flounder Paralichthys olivaceus
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Butaphosphan (BTP) has recently been introduced into the Korean aquaculture sector as a stressattenuating agent. In this study, a sensitive chemical analytical method was established for the detection of BTP in the olive flounder (Paralichthys olivaceus) tissues. Methods: Utilizing a method employing liquid chromatography coupled with tandem mass spectrometry (LC-MS/ MS), detection sensitivity, specificity, and precision were satisfactorily established. Temporal changes in the BTP plasma and muscle concentrations were assessed after a single intramuscular injection of BTP (50 and 150 mg/kg) to the olive flounder maintained at 13 °C or 22 °C. Results: High BTP plasma levels were achieved immediately after the injection, and the drug was rapidly eliminated. Additionally, plasma BTP levels were markedly dependent on the elimination rate, which, in turn, seemed dependent on the water temperature, with the drug elimination half-life and mean residence time significantly shorter at 22 °C than 13 °C. Overall, muscle BTP levels were markedly lower than the plasma levels. Notably, muscle levels were not influenced by water temperatures. Muscle BTP concentrations were used to estimate the necessary withdrawal period for drugs used in food fish, with BTP levels maintained far below the possible hazardous limit. Conclusions: In conclusion, the established LC-MS/MS method can be used for BTP residue detection with high sensitivity and reproducibility.
Abstract
Background: Butaphosphan (BTP) has recently been introduced into the Korean aquaculture sector as a stressattenuating agent. In this study, a sensitive chemical analytical method was established for the detection of BTP in the olive flounder (Paralichthys olivaceus) tissues. Methods: Utilizing a method employing liquid chromatography coupled with tandem mass spectrometry (LC-MS/ MS), detection sensitivity, specificity, and precision were satisfactorily established. Temporal changes in the BTP plasma and muscle concentrations were assessed after a single intramuscular injection of BTP (50 and 150 mg/kg) to the olive flounder maintained at 13 °C or 22 °C. Results: High BTP plasma levels were achieved immediately after the injection, and the drug was rapidly eliminated. Additionally, plasma BTP levels were markedly dependent on the elimination rate, which, in turn, seemed dependent on the water temperature, with the drug elimination half-life and mean residence time significantly shorter at 22 °C than 13 °C. Overall, muscle BTP levels were markedly lower than the plasma levels. Notably, muscle levels were not influenced by water temperatures. Muscle BTP concentrations were used to estimate the necessary withdrawal period for drugs used in food fish, with BTP levels maintained far below the possible hazardous limit. Conclusions: In conclusion, the established LC-MS/MS method can be used for BTP residue detection with high sensitivity and reproducibility.
참고문헌 (29)
- Alkobaby AI, "Effects of maternal injection with organic phosphorus and vitamin B12 on reproductive performance and newly hatched offspring of Nile tilapia (Oreochromis niloticus)", Proceedings, The 8th International Symposium on Tilapia in Aquaculture (2008)
- Benez LZ, "Pharmacokinetic parameters: which are necessary to define a drug substance?", Eur J Respir Dis Supp, 134, pp.45~61 (1984)
- Deniz A, "Efficacy of a butafosfan and vitamin B12 combination (Catosal®) on biochemical and hematological blood parameters in dogs treated with dexamethasone", Intern J Appl Res Vet Med, 7, pp.116~129 (2009)
- Du XY, "Studies on pharmacy and pharmacokinetics of compound butafosfan", Ph. D, pp.2004
- Durisova M, "A physiological review and structure of mean residence times", Gen Physiol Biophys, 33, pp.75~80 (2014)
- Opinion of the committee for medicinal products for veterinary use on the establishment of maximum residue limits: name of the substance-butafosfan, "European Medicines Agency", ~, pp.EMEA (2013), V[DOI]
- Funaki T, "Enterohepatic circulation model for population pharmacokinetic analysis", J Pharm Pharmacol, 51, pp.1143~1148 (1999)
- Fürll M, "Effect of multiple intravenous injections of butaphosphan and cyanocobalamin on the metabolism of periparturient dairy cows", J Dairy Sci, 93, pp.4155~4164 (2010)
- Fürll M, "Effects of preoperative application of butaphosphan and cyanocobalamin on reconvalescence, clinic-chemical parameters, and antioxidative metabolism and postoperative abomasal emptying in cows with abnormal dislocation", Tieraertl Prax, 34, pp.351~356 (2006)
- Gabrielsson J, "Pharmacokinetic and pharmacodynamic data analysis:concepts and applications", pp.645~659 (2000), Swedish Pharamceutical Press
- Hasi S, "Studies on effects of compound butaphosfan solution on endurance capability and energy metabolism in mice", Chin J Anim Vet Sci, 35, pp.290~294 (2004)
- Hasi S, "Anti-cold stress effects and mechanisms of compound butaphosphan solution", Prog Vet Med, 26, pp.59~62 (2005)
- Hasi S, "Studies on anti-heat stress effects and mechanisms of compound butaphosfan solution", Acta Vet Zootech Sin, 36, pp.1334~1338 (2005)
- Hekman P, "Withdrawal-time calculation program WT1.4", European agency for the evaluation of medicinal products (EMEA);, ~ (1996)
- Kleinow KM, "Comparative pharmacokinetics and bioavailability of oxolinic acid in channel catfish (Ictalurus punctatus) and rainbow trout (Oncorrhynchus mykiss)", Can J Fish Aquat Sci, 51, pp.1205~1211 (1994)
- "Principles of Standard Establishment for Foods" (2017)[URL]
- Korea Rural Economic Institute, "2017-Food Balance Sheet", ~ (2017)
- Mechesso AF, "Effects of butaphosphan and cyanocovalamin combination on plasma immune and biochemical parameters of olive flounder (Paralichthys olivaceus) subjected to crowding stress", Aquacult Res, 50, pp.1611~1617 (2019)
- Meyer RE, "Anesthesia and analgesia in laboratory animals", pp.27~82 (2008), Academic Press
- "Aquatic medicine catalogue" (2018)[URL]
- Pereira RA, "Metabolic parameters and dry matter intake of ewes treated with butaphosphan and cyanocobalamin in the early postpartum period", Small Rum Res, 114, pp.140~145 (2013)
- Pereira RA, "Effect of butaphosphan and cyanocobalamin on postpartum metabolism and milk production in dairy cows", Animal, 7, pp.1143~1147 (2013)
- Rolling E, "The effect of injectable butaphosphan and cyanocobalamin on postpartum serum betahydroxybutyrate, calcium, and phosphorus concentrations in dairy cattle", J Dairy Sci, 93, pp.978~987 (2010)
- Jung-Soo Seo, "Biochemical and stress-attenuating effects of butaphosphan-cyanocobalamin combination drug in olive flounder Paralichthys olivaceus", Fisheries Science, 86(2), pp.375~384 (2020), Springer Science and Business Media LLC[DOI]
- Sun F, "Pharmacokinetics of butafosfan after intravenous and intramuscular administration in piglets", J Vet Pharmacol Ther, 40, pp.203~205 (2016)
- Tabeleão VC, "Combined butaphosphan and cyanocobalamin on the glucose metabolism of dairy cows after calving", Arq Bras Med Vet Zootec, 69, pp.317~324 (2017)
- Test for potential protective effects of Catosal® on mortality of fish due to temporary hypoxia, "Van de Vis H", RIVO-Netherlands Institute for Fisheries Research, ~, pp.Internal Report No. C061 (2004), 04
- Van der Staay DE, "Effects of butaphosphan on salivary cortisol and behavioral response to social stress in piglets", J Vet Pharmacol Ther, 30, pp.410~416 (2007)
- Zhang Y, "PKSolver: an add-in program for pharmacokinetic and pharmacodynamics data analysis in Microsoft Excel", Computer Methods Programs Biomed, 99, pp.306~314 (2010)