酒蒸黃連이 iNOS 활성 억제를 통해 생쥐 위⋅십이지장 점막에 미치는 영향
Effect of Coptidis Rhizoma Steamed with Rice Wine on Gastroduodenal Mucosa of Mouse through Inhibiting iNOS Activation
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: This study was carried out to investigate the protective effect of Coptidis Rhizoma steamed with rice wine(CR) against gastroduodenal mucosal injury through inhibiting inducible nitric oxide synthase (iNOS) activation. Methods: In in vitro experiment, LPS-induced RAW 264.7 macrophages were treated with CR(0.4, 0.6, 0.8, 1.0 mg/ml)and iNOS mRNA expression and nitric oxide (NO) production were measured. In in vivo experiment normal group mice weretreated with neither ethanol nor CR. Both control and sample group mice were orally administrated with ethanol. Five hoursafter ethanol administration control group mice were orally administrated with distilled water, sample group mice were orallyadministrated with CR. After three days administration, gastroduodenal mucosa of mice was observed histopathologically andiNOS, nuclear factor-kappa B (NF-κB) activation were observed immunohistochemically. Results: In in vitro experiment iNOS mRNA expression and NO production in LPS-induced RAW 264.7 macrophages weredecreased by CR dose-dependently. In in vivo experiment, gastroduodenal mucosal injury was repaired by CR and iNOS, NF-κBactivation in gastroduodenal mucosa were decreased by CR. Conclusions: Coptidis Rhizoma steamed with rice wine has a protective effect against gastroduodenal mucosal injury throughinhibiting iNOS activation.
Abstract
Objectives: This study was carried out to investigate the protective effect of Coptidis Rhizoma steamed with rice wine(CR) against gastroduodenal mucosal injury through inhibiting inducible nitric oxide synthase (iNOS) activation. Methods: In in vitro experiment, LPS-induced RAW 264.7 macrophages were treated with CR(0.4, 0.6, 0.8, 1.0 mg/ml)and iNOS mRNA expression and nitric oxide (NO) production were measured. In in vivo experiment normal group mice weretreated with neither ethanol nor CR. Both control and sample group mice were orally administrated with ethanol. Five hoursafter ethanol administration control group mice were orally administrated with distilled water, sample group mice were orallyadministrated with CR. After three days administration, gastroduodenal mucosa of mice was observed histopathologically andiNOS, nuclear factor-kappa B (NF-κB) activation were observed immunohistochemically. Results: In in vitro experiment iNOS mRNA expression and NO production in LPS-induced RAW 264.7 macrophages weredecreased by CR dose-dependently. In in vivo experiment, gastroduodenal mucosal injury was repaired by CR and iNOS, NF-κBactivation in gastroduodenal mucosa were decreased by CR. Conclusions: Coptidis Rhizoma steamed with rice wine has a protective effect against gastroduodenal mucosal injury throughinhibiting iNOS activation.
참고문헌 (29)
- 대한내과학회 해리슨내과학 편집위원회, "Harrison’s 내과학", pp.1907~ (2006), 도서출판 MIP
- 한의과대학 본초학 편찬위원회, "본초학", pp.221~222 (2006), 도서출판 영림사
- 김기배, "黃連과 구성 생화합물의 약리작용에 대한 고찰", 대한한의학회지, 33(3), pp.160~183 (2012), 대한한의학회
- Li B, "Study of total alkaloids from Rhizoma Coptis Chinensis on experimental gastric ulcers", Chin J Integr Med, 11(3), pp.217~221 (2005)
- 변준석, "에탄올 유발 위점막 손상에 대한 황련 물 추출물의 방어효과", 동의생리병리학회지, 26(1), pp.67~73 (2012), 한의병리학회
- Lu JS, "Protective effects and its mechanisms of total alkaloids from rhizoma Coptis chinensis on Helicobacter pylori LPS induced gastric lesion in rats", Zhongguo Zhong Yao Za Zhi, 32(13), pp.1333~1336 (2007)
- 허준, "동의보감", pp.140~2157 (2006), 동의보감 출판사
- 전국한의과대학 비계내과학교실, "비계내과학", pp.79~213 (2008), 군자출판사
- Li JC, "A traditional Chinese medicine JiuHuangLian (Rhizoma coptidis steamed with rice wine) reduces oxidative stress injury in type 2 diabetic rats", Food Chem Toxicol, 59, pp.222~229 (2013)
- Nussler AK, "Inflammation, immunoregulation, and inducible nitric oxide synthase", J Leukoc Biol, 54(2), pp.171~178 (1993)
- Elliott SN, "Nitric oxide: a regulator of mucosal defense and injury", J Gastroenterol, 33(6), pp.792~803 (1998)
- 박용기, "黃連의 주성분인 Berberine의 뇌신경소교세포로부터 LPS에 의해 유도되는 염증매개물질 생성억제효과", 대한본초학회지, 22(4), pp.117~125 (2007), 대한본초학회
- Zhang Q, "Preventive effect of Coptis chinensis and berberine on intestinal injury in rats challenged with lipopolysaccharides", Food Chem Toxicol, 49(1), pp.61~69 (2011)
- 박용기, "황련 클로로포름 분획물의 뇌신경소교세포로부터 염증매개물질 생성억제 효능 연구", 대한본초학회지, 22(4), pp.109~116 (2007), 대한본초학회
- 박용기, "황련의 쥐 대식세포로부터 LPS에 의해 유도되는 nitric oxide 및 TNF-α의 생성억제효과", 대한본초학회지, 21(2), pp.165~173 (2006), 대한본초학회
- 박용기, "황련 추출물의 분획화 및 BV2 microglial cells에서 LPS에 의해 유도되는 nitric oxide 생성억제효과 검정", 대한본초학회지, 22(2), pp.73~78 (2007), 대한본초학회
- Tak PP, "NF-κB: a key role in inflammatory diseases", J Clin Invest, 107(1), pp.7~11 (2001)
- Aktan F, "iNOS-mediated nitric oxide production and its regulation", Life Sciences, 75(6), pp.639~653 (2004)
- Xie QW, "Role of Transcription Factor NF-κB", Rel in Induction of Nitric Oxide Synthase, 1994, pp.269(7), 4705~4708
- van Den Brink GR, "Expression and activation of NF-κB in the antrum of the human stomach", J Immunol, 164(6), pp.3353~3359 (2000)
- Neurath MF, "Cytokine gene transcription by NF-kappa B family members in patients with inflammatory bowel disease", Ann N Y Acad Sci, 859, pp.149~159 (1998)
- Moorchung N, "Nuclear factor kappa-B and histopathology of chronic gastritis", Indian J Pathol Microbiol, 53(3), pp.418~421 (2010)
- Zhang Q, "Preventive effect of Coptis chinensis and berberine on intestinal injury in rats challenged with lipopolysaccharides", Food and Chemical Toxicology, 49(1), pp.61~69 (2011)
- Prasad K, "Image analysis tools for evaluation of microscopic views of immunohistochemically stained specimen in medical research-a review", J Med Syst, 36(4), pp.2621~2631 (2012)
- Knoll MR, "Action of pure ethanol and some alcoholic beverages on the gastric mucosa in healthy humans: A descriptive endoscopic study", Endoscopy, 30(3), pp.293~301 (1998)
- Gottfried EB, "Alcohol -induced Gastric and Duodenal Lesions in Men", Am J Gastroenterol, 70(6), pp.587~592 (1978)
- McManus JFA, "Histological and Histochemical Uses of Periodic Acid", Stain Technol, 23(3), pp.99~108 (1948)
- Bry L, "Paneth cell differentiation in the developing intestine of normal and transgenic mice", Proc Natl Acad Sci USA, 91(22), pp.10335~10339 (1994)
- Lee JS, "A study on the Extraction Efficiency of berberine in Coptidis Rhizoma", Analytical Science & Technology, 10(2), pp.83~90 (1997)