Indomethacin으로 유발된 생쥐의 위점막 손상에 대한 消滯丸의 효과
Effect of Socheh-wan Extract on Indomethacin-induced Gastric Mucosal Lesions in Mice
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Objectives : This study was carried out to investigate the effects of Soche-hwan extract on indomethacin-induced gastric mucosal lesions of mice. Methods : Experimental mice were divided randomly into four groups. The normal group, the gastropathy group of gastro-inflammation elicited mice, the misoprostol group of mice administered misoprostol after gastro-inflammation elicitation and the Soche-hwan group of mice administered Soche-hwan after gastro-inflammation elicitation. Results : The hemorrhagic erosion of gastric mucosa, the damage of arrangement of mucous secreted cells and HSP70 increased in the gastro-inflammation elicited group, but decreased significantly in the misoprostol and Soche-hwan extract administered groups. Cell proliferation of gastric mucosa decreased in the gastro-inflammation elicited group, but increased significantly in the misoprostol and Soche-hwan extract administered groups. The distribution of mucosal neck cells and mucosal surface cells and PNA positive reactions of surface mucus cells decreased in the gastro-inflammation elicited group, but increased significantly in the misoprostol and Soche-hwan extract administered groups. COX-1 positive cells decreased in the gastro-inflammation elicited group, but increased significantly in the misoprostol and Soche-hwan extract administered groups. iNOS mRNA and COX-2 mRNA increased in the gastro-inflammation elicited group, but decreased significantly in the Soche-hwan extract administered group. NF-kB p65, iNOS and COX-2 increased in the gastro-inflammation elicited group, but decreased significantly in the misoprostol and Soche-hwan extract administered groups. Conclusion : Soche-hwan extract had excellent effects on indomethacin-induced gastric mucosal lesions in mice.
Abstract
Objectives : This study was carried out to investigate the effects of Soche-hwan extract on indomethacin-induced gastric mucosal lesions of mice. Methods : Experimental mice were divided randomly into four groups. The normal group, the gastropathy group of gastro-inflammation elicited mice, the misoprostol group of mice administered misoprostol after gastro-inflammation elicitation and the Soche-hwan group of mice administered Soche-hwan after gastro-inflammation elicitation. Results : The hemorrhagic erosion of gastric mucosa, the damage of arrangement of mucous secreted cells and HSP70 increased in the gastro-inflammation elicited group, but decreased significantly in the misoprostol and Soche-hwan extract administered groups. Cell proliferation of gastric mucosa decreased in the gastro-inflammation elicited group, but increased significantly in the misoprostol and Soche-hwan extract administered groups. The distribution of mucosal neck cells and mucosal surface cells and PNA positive reactions of surface mucus cells decreased in the gastro-inflammation elicited group, but increased significantly in the misoprostol and Soche-hwan extract administered groups. COX-1 positive cells decreased in the gastro-inflammation elicited group, but increased significantly in the misoprostol and Soche-hwan extract administered groups. iNOS mRNA and COX-2 mRNA increased in the gastro-inflammation elicited group, but decreased significantly in the Soche-hwan extract administered group. NF-kB p65, iNOS and COX-2 increased in the gastro-inflammation elicited group, but decreased significantly in the misoprostol and Soche-hwan extract administered groups. Conclusion : Soche-hwan extract had excellent effects on indomethacin-induced gastric mucosal lesions in mice.
참고문헌 (44)
- 대한소화기학회, "소화성궤양", pp.1~ (2002), 군자출판사
- 全國韓醫科大學 脾系內科學校室, "脾系內科學", pp.239~240 (1998), 아트동방
- 국민건강보험공단, "건강보험통계연보", 368~ (2005)
- 이동필, "한국인의 소화성궤양에 대한 임상적 고찰", 대한내과학회지, 48, pp.773~782 (1995)
- 해리슨 내과학 편찬위원회, "해리슨 내과학", pp.1466~1485 (1997), 정담
- 新谷太, "Steps to internal medicine pathophysiology로 이해하는 내과학 소화기질환(6)", pp.63~79 (2002), 정담
- 서울대학교 의과대학, "소화기학원론", pp.46~ (1998), 서울대학교출판부
- 윤종구, "소화성 궤양에 대한 임상적 고찰", 대한소화기학회지, 36, pp.336~347 (2000)
- 윤봉영, "비스테로이드성 항염증제제에 의한 급성 위점막 손상에 있어 호중구의 역할", 대한소화기학회지, 35, pp.154~162 (2000)
- 陳貴廷, "實用中西醫結合診斷治療學", pp.437~ (1992), 一中社
- 危北海, "中醫脾胃學說應用硏究", pp.203~210 (1993), 北京出版社
- 허준, "동의보감", pp.1208~ (2005), 동의보감출판사
- 손정숙, "四君子湯合蒼朮地楡湯이 위점막손상에 미치는 영향", 대한한방내과학회지, 20(2), pp.231~248 (1999)
- 김정룡, "소화기계질환", pp.209~ (2005), 일조각
- 김갑성, "實用東西醫學臨床總書", pp.453~555 (2001), 정담
- 康秉秀, "本草學", pp.252-4~ (1998), 永林社
- 한방약리학 교재편찬위원회, "한방약리학", pp.532-4~ (2006), 도서출판 신일상사
- Mitchell JA, "Cyclooxygenase-2: Pharmacology, physiology, biochemistry and relevance to NSAID therapy", British J Pharmacol 128, 1121~1131 (1999)
- 김경환, "이우주의 약리학 강의", pp.719~728 (1997), 의학문화사
- 권오윤, "실험적으로 일으킨 위궤양 발생에 대한 Misoprostol(PG E2)의 보호효과에 대한 연구", 대한소화기병학회지, 22(4), pp.44~45 (1990)
- David Y Graham, "NSAID ulcers : prevalence and prevention", Mod Rheumatol, 10, pp.2~7 (2000)
- Schoen R T, "Mechanism of NASID-induced gastric damage", AM, J Med, 86, pp.449~458 (1989)
- Wallace, J L, "The cellular and molecular basis of gastric mucosal defense", FASEB, 10, pp.731~740 (1996)
- Wallace, J L, "Gastric ulceration:critical eventsat the neutrophil-endothelium interface", Can J Physiol Pharmacol., 71, pp.98~102 (1993)
- Yi, L, "Behavioral and physiological correlated of activity stress-induced ulcers", critical review. Exp. Neurobio, 4, pp.61~80 (1995)
- De Maio A, "The heat-shock response", New Horiz, 2, pp.198~207 (1995)
- Gonzalez M F, "Heat shock protein as markers of neuronal injury", Mol. Brain Res, 6, pp.93~100 (1989)
- Vass K, "Induction of stress protein HSP70 in nerve cell after status epilepticus in the rat", Neurosci Lett, 100, pp.259~264 (1989)
- Bergstedt K, "Initiation of protein synthesis and heat shock protein-72 expression in the rat brain following sereve insulin induced hypoglycemia", Acta Neuropathol, 86, pp.259~264 (1989)
- Valazquez J M, "Hsp 70- Nuclear concentration during enviromental stress and cytoplasmic stroage during recovery", Cell, 36, pp.622~655 (1984)
- Maulik N, "Drug-induced heat-shock preconditioning improves postischemic ventricular recovery after cardiopulmonary bypass", Circulation, 92, pp.381~388 (1995)
- Maulik N, "Improvedpostischemic ventricular functional recovery by amphetamine is linked with its ability to induce heat shock", Mol cell Biochem, 137, pp.17~24 (1994)
- Criag E A, "Heat shock protein:molecular chaperone of protein biogenesis", Microbiol Rev., 57, pp.402~414 (1993)
- Tetsuo Arakawa, "Prostaglandins in the stomach: an update", J Clin Gastroenterol., 27, pp.1~11 (1998)
- Byron Cryer, "The advent of highly selective inhibitors of cyclooxygenase-a review", Prostaglandins & other Lipid Mediators, 58, pp.341~361 (1998)
- Lee S Siomon, "Biology and toxic effects of nonsteroidal anti-inflammatory drugs", Current Opinion in Rheumatology, 10, pp.153~158 (1998)
- Byron Cryer, "Gastrointestinal side effects of nonsteroidal anti-inflammatory drug", Am J med, 105, pp.20~30 (1998)
- Choitsu Sakamoto, "Roles of COX-1 and COX-2 in gastrointestinal pathophysiology", J Gastroenterol, 33, pp.618~624 (1998)
- Martin Kulldorff, "Measuring cell proliferation in rectal mucosa:Comparing bromodeoxyuridine(BrdU) and proliferating cell nuclear antigen(PCNA) assays", J Clin Epidem., 53, pp.875~883 (2000)
- Baeuerle P A, "NF-κB-Ten years after", Cell, 87, pp.13~20 (1996)
- Baeuerle P A, "IκB-NF-κB structure : at the interface of inflammation control", Cell, 95, pp.729~731 (1998)
- Anggard E, "Nitric oxide: mediator, murderer, and medicine", Lancet, 9, pp.1199~1206 (1994)
- Radi R, "Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide", Arch Biochem Biophys, 288(2), pp.481~487 (1991)
- Villa L M, "Peroxynitrite induces both vasodilatation and impaired vascular relaxation in the isolated perfused rat heart", Proc Natl Acad Sci USA., 91(26), pp.12383~12387 (1994)