Accumulation, Elimination and Cell Response in the Kidney of Juvenile Rockfish (Sebastes schlegeli) Exposed to Dietary Cadmium
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Abstract
Experiments were carried out to investigate Cd accumulation, elimination and cell response in juvenile rockfish (Sebastes schlegeli) exposed to sub-chronic dietary Cd (0, 0.5, 5, 25 and 125mg/kg) for 60days and depuration periods of 30days. Cd accumulation in the kidney of rockfish increased with exposure periods and concentrations for the 60days of dietary Cd exposure. After the end of the dietary Cd exposure, Cd accumulation values in the kidney were 52.9±9.94μg/g and 90.6±15.7μg/g for those exposed to 25mg/kg and 125mg/kg Cd, respectively. The accumulation factors increased with the exposure period in the kidney. Cd elimination in the kidney of rockfish did not vary significantly and remained constant after the cessation of the dietary Cd exposure. In the primary exposure periods, the effect of kidney tissue in the rockfish exposed to dietary Cd was observed the swelling of capillary of the glomerulus. In addition, there was also hydropic swelling within the pyknotic nuclei, some of hyaline droplet accumulation and the microvilli showed a positive reaction to alcian blue in the tubular cells. While exposure time and concentrations were increased, there was a lot of hyaline droplet accumulation and the microvilli showed a positive reaction to alcian blue in the tubular cells. Fused renal tubule and its necrosis were observed after 60days at 125mg/kg.
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