Comparative analysis of the pig gut microbiome associated with the pig growth performance
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
There are a variety of microorganisms in the animal intestine, and it has been known that they play important roles in the host such as suppression of potentially pathogenic microorganisms, modulation of the gut immunity. In addition, the gut microbiota and the livestock growth performance have long been known to be related. Therefore, we evaluated the interrelation between the growth performance and the gut microbiome of the pigs from 3 different farms, with pigs of varied ages ready to be supplied to the market. When pigs reached average market weight of 118 kg, the average age of pigs in three different farms were < 180 days, about 190 days, and > 200 days, respectively. Fecal samples were collected from pigs of age of 70 days, 100 days, 130 days, and 160 days. The output data of the 16S rRNA gene sequencing by the Illumina Miseq platform was filtered and analyzed using Quantitative Insights into Microbial Ecology (QIIME)2, and the statistical analysis was performed using Statistical Analysis of Metagenomic Profiles (STAMP). The results of this study showed that the gut microbial communities shifted as pigs aged along with significant difference in the relative abundance of different phyla and genera in different age groups of pigs from each farm. Even though, there was no statistical differences among groups in terms of Chao1, the number of observed operational taxonomic units (OTUs), and the Shannon index, our results showed higher abundances of Bifidobacterium, Clostridium and Lactobacillus in the feces of pigs with rapid growth rate. These results will help us to elucidate important gut microbiota that can affect the growth performance of pigs.
Abstract
There are a variety of microorganisms in the animal intestine, and it has been known that they play important roles in the host such as suppression of potentially pathogenic microorganisms, modulation of the gut immunity. In addition, the gut microbiota and the livestock growth performance have long been known to be related. Therefore, we evaluated the interrelation between the growth performance and the gut microbiome of the pigs from 3 different farms, with pigs of varied ages ready to be supplied to the market. When pigs reached average market weight of 118 kg, the average age of pigs in three different farms were < 180 days, about 190 days, and > 200 days, respectively. Fecal samples were collected from pigs of age of 70 days, 100 days, 130 days, and 160 days. The output data of the 16S rRNA gene sequencing by the Illumina Miseq platform was filtered and analyzed using Quantitative Insights into Microbial Ecology (QIIME)2, and the statistical analysis was performed using Statistical Analysis of Metagenomic Profiles (STAMP). The results of this study showed that the gut microbial communities shifted as pigs aged along with significant difference in the relative abundance of different phyla and genera in different age groups of pigs from each farm. Even though, there was no statistical differences among groups in terms of Chao1, the number of observed operational taxonomic units (OTUs), and the Shannon index, our results showed higher abundances of Bifidobacterium, Clostridium and Lactobacillus in the feces of pigs with rapid growth rate. These results will help us to elucidate important gut microbiota that can affect the growth performance of pigs.
참고문헌 (20)
- Hyun-Woo Cho, "Gut microbiota profiling in aged dogs after feeding pet food contained Hericium erinaceus", 한국축산학회지, 64(5), pp.937~949 (2022), 한국축산학회
- Luckey TD, "Introduction to intestinal microecology", Am J Clin Nutr, 25, pp.1292~1294 (1972)[DOI]
- Savage DC, "Microbial ecology of the gastrointestinal tract", Annu Rev Microbiol, 31, pp.107~133 (1977)[DOI]
- Berg RD, "The indigenous gastrointestinal microflora", Trends Microbiol, 4, pp.430~435 (1996)[DOI]
- Isaacson R, "The intestinal microbiome of the pig", Anim Health Res Rev, 13, pp.100~109 (2012)[DOI]
- Kim HB, "Longitudinal investigation of the age-related bacterial diversity in the feces of commercial pigs", Vet Microbiol, 153, pp.124~133 (2011)[DOI]
- 김민지, "Fecal microbiome shifts by different forms of copper supplementations in growing pigs", 한국축산학회지, 63(6), pp.1386~1396 (2021), 한국축산학회[DOI]
- Hanshew AS, "Minimization of chloroplast contamination in 16S rRNA gene pyrosequencing of insect herbivore bacterial communities", J Microbiol Methods, 95, pp.149~155 (2013)[DOI]
- Guevarra RB, "The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition", J Anim Sci Biotechnol, 9, pp.54~ (2018)[DOI]
- Navas-Molina JA, "Advancing our understanding of the human microbiome using QIIME", Methods Enzymol, 531, pp.371~444 (2013)[DOI]
- Lu D, "Host contributes to longitudinal diversity of fecal microbiota in swine selected for lean growth", Microbiome, 6, pp.4~ (2018)[DOI]
- Tan Z, "Metagenomic analysis of cecal microbiome identified microbiota and functional capacities associated with feed efficiency in landrace finishing pigs", Front Microbiol, 8, pp.1546~ (2017)[DOI]
- Pedersen R, "Characterisation of gut microbiota in Ossabaw and Göttingen minipigs as models of obesity and metabolic syndrome", PLOS ONE, 8, pp.e56612~ (2013)[DOI]
- Guevarra RB, "Oral vaccination against Lawsonia intracellularis changes the intestinal microbiome in weaned piglets", Animals, 11, pp.2082~ (2021)[DOI]
- Law K, "Disinfection of maternal environments is associated with piglet microbiome composition from birth to weaning", mSphere, 6 (2021)[DOI]
- Mikkelsen LL, "Enumeration of bifidobacteria in gastrointestinal samples from piglets", Appl Environ Microbiol, 69, pp.654~658 (2003)[DOI]
- Hermann-Bank ML, "The Gut Microbiotassay : a high-throughput qPCR approach combinable with next generation sequencing to study gut microbial diversity", BMC Genomics, 14, pp.788~ (2013)[DOI]
- Verschuren LMG, "Fecal microbial composition associated with variation in feed efficiency in pigs depends on diet and sex", J Anim Sci, 96, pp.1405~1418 (2018)[DOI]
- Blander JM, "Regulation of inflammation by microbiota interactions with the host", Nat Immunol, 18, pp.851~860 (2017)[DOI]
- Robie Vasquez, "Gut microbiome-produced metabolites in pigs: a review on their biological functions and the influence of probiotics", 한국축산학회지, 64(4), pp.671~695 (2022), 한국축산학회