Measurement of Antioxidant Activities and Phenolic and Flavonoid Contents of the Brown Seaweed Sargassum horneri: Comparison of Supercritical CO2 and Various Solvent Extractions
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Seaweed Sargassum horneri extracts were obtained using supercritical carbon dioxide (SC-CO2) and different solvents. SC-CO2 was kept at a temperature of 45ºC and pressure of 250 bar. The flow rate of CO2 (27 g/min) was constant during the entire 2-h extraction period, and ethanol was used as a cosolvent. Six different solvents [acetone, hexane, methanol, ethanol, acetone mix methanol (7:3), and hexane mix ethanol (9:1)] were used for extraction and agitated by magnetic stirring (250 rpm) in the dark at 25ºC for 20 h; the ratio of material to solvent was 1:10 (w/v). Antioxidant properties of S. horneri extracted using SC-CO2 with ethanol and different solvents have shown good activity. The highest activity belongs to SC-CO2 with ethanol extracted oil, showing DPPH, ABTS, total phenolic content, and total flavonoid levels of 68.38 ± 1.21%, 83.51 ± 1.25%, 0.64 ± 0.02 mg/g, and 5.57 ± 0.05 mg/g, respectively. The S. horneri extracts showed a significant correlation between the antioxidant activity and phenolic content. Based on these results, the SC-CO2 extract (ethanol) of the seaweed extract from brown seaweed may be a valuable antioxidant source.
Abstract
Seaweed Sargassum horneri extracts were obtained using supercritical carbon dioxide (SC-CO2) and different solvents. SC-CO2 was kept at a temperature of 45ºC and pressure of 250 bar. The flow rate of CO2 (27 g/min) was constant during the entire 2-h extraction period, and ethanol was used as a cosolvent. Six different solvents [acetone, hexane, methanol, ethanol, acetone mix methanol (7:3), and hexane mix ethanol (9:1)] were used for extraction and agitated by magnetic stirring (250 rpm) in the dark at 25ºC for 20 h; the ratio of material to solvent was 1:10 (w/v). Antioxidant properties of S. horneri extracted using SC-CO2 with ethanol and different solvents have shown good activity. The highest activity belongs to SC-CO2 with ethanol extracted oil, showing DPPH, ABTS, total phenolic content, and total flavonoid levels of 68.38 ± 1.21%, 83.51 ± 1.25%, 0.64 ± 0.02 mg/g, and 5.57 ± 0.05 mg/g, respectively. The S. horneri extracts showed a significant correlation between the antioxidant activity and phenolic content. Based on these results, the SC-CO2 extract (ethanol) of the seaweed extract from brown seaweed may be a valuable antioxidant source.
참고문헌 (32)
- Adedapo AA, "Evaluation of the medicinal potentials of the methanol extracts of the leaves and stems of Halleria lucida", Bio Resour Technol, 99, pp.4158~4163 (2008)
- American Oil Chemists’ Society, "Official methods of analysis" (1998), American Oil Chemists’ Society
- Athukorala Y, "Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea", Bio Resour Techno, 98, pp.1711~1716 (2007)
- Blunt JW, "Marine natural products", Nat Prod Rep, 28, pp.196~268 (2011)
- Burtin P, "Nutritional value of seaweeds", Electron J Environ Agric Food Chem, 2, pp.498~503 (2003)
- Cai YZ, "Structure-radical scavenging activity relationships of phenolic compounds from traditional chinese medical plants", Life Sci, 78, pp.2872~2888 (2004)
- Chakraborty K, "Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae)collected from Gulf of Mannar", Asian Pac J Trop Biomed, 3, pp.8~16 (2013)
- Chandini SK, "In Vitro antioxidant activities of three selected brown seaweeds of India", Food Chem, 107, pp.707~713 (2008)
- Chew YL, "Antioxidant activity of three edible seaweeds from two areas in South East Asia", LWT - Food Sci Technol, 41, pp.1067~1072 (2008)
- Conde E, "Supercritical CO2 extraction of fatty acids, phenolics and fucoxanthin from freeze-dried Sargassum muticum", J App Phycol, 27, pp.957~964 (2014)
- Crespo MOP, "Determination of aliphatic hydrocarbons in the alga. Himanthalia Elongate", Ecotoxicol Environ Saf, 57, pp.226~230 (2004)
- Devi KP, "Bioprotective properties of seaweeds : In vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content", BMC Complem M, 8, pp.38~ (2008)
- Do QD, "Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica", JFDA, 22, pp.296~302 (2014)
- Essien EA, "Preparation and evaluation of Cucumeropsis mannii naud seed oil metallic soaps as driers in gloss paint", J Mater Environ Sci, 3, pp.477~484 (2012)
- Essien EA, "Preparation and evaluation of Cucumeropsis manniinaud seed oil metallic soaps as driers in gloss paint", J Mater Environ Sci, 3, pp.477~484 (2012)
- Hong YL, "Evaluation of antioxidant activities of five selected brown seaweeds from China", J Med Plants Res, 4, pp.2557~2565 (2010)
- Jose L, "Supercritical fluid extraction of nutraceuticals and bioactive compounds", pp.2008, CRC Press
- Kamal-Eldin A, "N-3 fatty acid for human nutrition : stability considerations", Eur J Lipid Sci Technol, 104, pp.825~836 (2002)
- Koivikko R, "Contents of soluble, cell-wall-bound and exuded phlorotannins in the brown alga Fucus vesiculosus, with implications on their ecological functions", J Chem Eco, 31, pp.195~212 (2005)
- Lang Q, "Supercritical fluid extraction in herbal and natural product studies— A practical review", Talanta, 53, pp.771~782 (2001)
- Li HB, "Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants", Lwt-Food Sci Technol, 41, pp.385~390 (2008)
- Macartain P, "Nutritional value of edible seaweeds", Nutr Res, 65, pp.535~543 (2007)
- Ozsoy N, "Antioxidant activity of Smilax Excelsa L. leaf extracts", Food Chem, 110, pp.571~583 (2007)
- Pandithurai M, "Free radical scavenging activity of methanolic extract of brown alga", J chem pharm res, 6, pp.128~132 (2014)
- Park PJ, "Antioxidant activities of enzymatic extracts from an edible seaweed Sargassum horneri using ESR spectrometry", J Food Lipids, 11, pp.15~27 (2004)
- Ping S, "Response surface optimization of wheat germ oil yield by supercritical carbon dioxide extraction", J Food Bioprod Process, 86, pp.227~231 (2008)
- Rogalinski T, "Hydrolysis kinetics of biopolymers in subcritical water", J Supercrit Fluids, 46, pp.335~341 (2008)
- 노명균, "Extraction of Fucoxanthin and Polyphenol from Undaria pinnatifida Using Supercritical Carbon Dioxide with Co-solvent", Biotechnology and Bioprocess Engineering, 13(6), pp.724~729 (2008), 한국생물공학회
- Shao P, "Chemical characterization, antioxidant and antitumor activity of sulfated polysaccharide from Sargassum horneri", Carbo Poly, 105, pp.260~269 (2014)
- Steinberg PD, "Lack of avoidance of phenolic-rich brown algae by tropical herbivorous fishes", Mar Biol, 109, pp.335~343 (1991)
- Vairappan CS, "Antibacterial halogenated metabolites from the Malaysian Laurencia species", Phytochemistry, 58, pp.291~297 (2001)
- Zhang WW, "Evaluation of 28 marine algae from the Qingdao coast for antioxidative capacity and determination of antioxidant efficiency and total phenolic content of fractions and sub fractions derived from Symphyocladia latiuscula (Rhodomelaceae)", J Appl Phycol, 19, pp.97~108 (2007)