사육 밀도가 무지개송어(Oncorhynchus mykiss) 치어의 성장, 체조성, 스트레스, 항산화 및 면역반응에 미치는 영향
Effects of Stocking Density on the Growth, Body Composition, Stress, Antioxidant Status and Immune Responses of Juvenile Rainbow Trout Oncorhynchus mykiss
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A 12-week feeding trial was conducted to evaluate the effect of different stocking densities on the growth, body composition, stress, antioxidant status, and immune responses of juvenile rainbow trout Oncorhynchus mykiss. Juvenile rainbow trout (32.2±0.5 g) were randomly placed in eight experimental tanks, each containing 2,000 L of water, at stocking densities of 150, 300, 600, and 1,200 individual/m3. The trout were assigned to four treatments (2.4, 4.8, 9.7, and 19.4 kg/m3) in duplicates. Following after the feeding trial, the fish cultured in the less dense group showed significantly higher growth and survival (P<0.05). In terms of proximate composition, the whole body crude protein and lipid contents were lower in the denser group (19.4 kg/m3). Regarding the antioxidant and immune responses, the fish reared in the relatively denser group exhibited lower lysozyme, immunoglobulin M, and superoxide dismutase levels. The stress indicators, glucose and cortisol, were significantly lower in the less dense group (2.4 kg/m3), while the denser group (19.4 km/m3) exhibited higher AST and ALP levels(P<0.05). The results showed that denser stocking has a crowding stress effect and negatively impacts growth and immune responses.
Abstract
A 12-week feeding trial was conducted to evaluate the effect of different stocking densities on the growth, body composition, stress, antioxidant status, and immune responses of juvenile rainbow trout Oncorhynchus mykiss. Juvenile rainbow trout (32.2±0.5 g) were randomly placed in eight experimental tanks, each containing 2,000 L of water, at stocking densities of 150, 300, 600, and 1,200 individual/m3. The trout were assigned to four treatments (2.4, 4.8, 9.7, and 19.4 kg/m3) in duplicates. Following after the feeding trial, the fish cultured in the less dense group showed significantly higher growth and survival (P<0.05). In terms of proximate composition, the whole body crude protein and lipid contents were lower in the denser group (19.4 kg/m3). Regarding the antioxidant and immune responses, the fish reared in the relatively denser group exhibited lower lysozyme, immunoglobulin M, and superoxide dismutase levels. The stress indicators, glucose and cortisol, were significantly lower in the less dense group (2.4 kg/m3), while the denser group (19.4 km/m3) exhibited higher AST and ALP levels(P<0.05). The results showed that denser stocking has a crowding stress effect and negatively impacts growth and immune responses.
참고문헌 (60)
- C E Adams, "Multiple determinants of welfare in farmed fish:Stocking density, disturbance, and aggression in atlantic salmon (Salmo salar)", Canadian Journal of Fisheries and Aquatic Sciences, 64(2), pp.336~344 (2007), Canadian Science Publishing[DOI]
- Ercüment Aksakal, "Effects of hypoxia and hyperoxia on growth parameters and transcription levels of growth, immune system and stress related genes in rainbow trout", Comp Biochem Physiol Part A Mol Integr Physiol, 262, pp.111060~ (2021), Elsevier BV[DOI]
- Ercüment Aksakal, "Increasing stocking density causes inhibition of metabolic–antioxidant enzymes and elevates mRNA levels of heat shock protein 70 in rainbow trout", Livestock Science, 141(1), pp.69~75 (2011), Elsevier BV[DOI]
- Ardiansyah, "Water quality, growth and stress responses of juvenile barramundi (Lates calcarifer Bloch), reared at four different densities in integrated recirculating aquaculture systems", Aquaculture, 458, pp.113~120 (2016), Elsevier BV[DOI]
- Official methods of analysis of AOAC International, "AOAC (Association of Official Analytical Chemists)", AOAC, ~ (2000)
- B. A. Barton, "Stress in Fishes: A Diversity of Responses with Particular Reference to Changes in Circulating Corticosteroids", Integrative and Comparative Biology, 42(3), pp.517~525 (2002), Oxford University Press (OUP)[DOI]
- T. V. Basrur, "Effects of repeated crowding on the stress response and growth performance in Atlantic salmon (Salmo salar)", Fish Physiology and Biochemistry, 36(3), pp.445~450 (2010), Springer Science and Business Media LLC[DOI]
- C. Binuramesh, "Diel variations in the selected serum immune parameters in Oreochromis mossambicus", Fish Shellfish Immunol, 30(3), pp.824~829 (2011), Elsevier BV[DOI]
- S. E. Wendelaar Bonga, "The stress response in fish", Physiological Reviews, 77(3), pp.591~625 (1997), American Physiological Society[DOI]
- Jeong-Hyeon Cho, "Effects of black soldier fly (Hermetia illucens) pre-pupae meal on the growth, stress, and immune responses of juvenile rainbow trout (Oncorhynchus mykiss) reared at different stocking densities", Aquaculture Reports, 25, pp.101202~ (2022), Elsevier BV[DOI]
- Jeong-Hyeon Cho, "A preliminary study of dietary protein requirement of juvenile marbled flounder (Pseudopleuronectes yokohamae)", Animal Nutrition, 7(2), pp.548~555 (2021), Elsevier BV[DOI]
- Aziza T.F. EL-Khaldi, "Effect of different stress factors on some physiological parameters of Nile tilapia (Oreochromis niloticus)", Saudi Journal of Biological Sciences, 17(3), pp.241~246 (2010), Elsevier BV[DOI]
- Ellis T, "The relationships between stocking density and welfare in farmed rainbow trout", J Fish Biol, 61, pp.493~531 (2002)[DOI]
- "The state of world fisheries and aquaculture 2022" (2022)[URL]
- Håstein T, "Animal welfare issues relating to aquaculture", OIE Global Conference on Animal Welfare, pp.219~227 (2004)
- Harsha Haridas, "Enhanced growth and immuno-physiological response of Genetically Improved Farmed Tilapia in indoor biofloc units at different stocking densities", Aquaculture Research, 48(8), pp.4346~4355 (2017), Hindawi Limited[DOI]
- V. de las Heras, "Influence of stocking density on growth, metabolism and stress of thick- lipped grey mullet (Chelon labrosus) juveniles", Aquaculture, 448, pp.29~37 (2015), Elsevier BV[DOI]
- M. Herrera, "Stocking density affects growth and metabolic parameters in the brill (Scophthalmus rhombus)", Aquaculture International, 20(6), pp.1041~1052 (2012), Springer Science and Business Media LLC[DOI]
- Jun-ichi Hikima, "Immunoglobulin genes and their transcriptional control in teleosts", Dev Comp Immunol, 35(9), pp.924~936 (2011), Elsevier BV[DOI]
- H.K. Hwang, "Effects of stocking density on the cage culture of Korean rockfish (Sebastes schlegeli)", Aquaculture, 434, pp.303~306 (2014), Elsevier BV[DOI]
- Rui Jia, "Stress and immune responses in skin of turbot (Scophthalmus maximus) under different stocking densities", Fish Shellfish Immunol, 55, pp.131~139 (2016), Elsevier BV[DOI]
- "Fishery Production Survey" (2022)[URL]
- Raúl Laiz-Carrión, "Influence of food deprivation and high stocking density on energetic metabolism and stress response in red porgy, Pagrus pagrus L", Aquaculture International, 20(3), pp.585~599 (2011), Springer Science and Business Media LLC[DOI]
- Baoliang Liu, "The effect of stocking density on growth and seven physiological parameters with assessment of their potential as stress response indicators for the atlantic salmon (Salmo salar)", Marine and Freshwater Behaviour and Physiology, 48(3), pp.177~192 (2015), Informa UK Limited[DOI]
- Gang Liu, "Influence of stocking density on growth, digestive enzyme activities, immune responses, antioxidant of Oreochromis niloticus fingerlings in biofloc systems", Fish Shellfish Immun, 81, pp.416~422 (2018), Elsevier BV[DOI]
- Lina Long, "Effects of stocking density on growth, stress, and immune responses of juvenile Chinese sturgeon (Acipenser sinensis) in a recirculating aquaculture system", Comp Biochem Phyiol Part C Toxicol Pharmacol, 219, pp.25~34 (2019), Elsevier BV[DOI]
- S. MacKenzie, "Transcriptional analysis of LPS-stimulated activation of trout (Oncorhynchus mykiss) monocyte", macrophage cells in primary culture treated with cortisol, 2006, pp.43(9), 1340~1348[DOI]
- Leonardo J. Magnoni, "Hypoxia, but not an electrolyte-imbalanced diet, reduces feed intake, growth and oxygen consumption in rainbow trout (Oncorhynchus mykiss)", Scientific Reports, 8(1), pp.4965~ (2018), Springer Science and Business Media LLC[DOI]
- Leonardo J. Magnoni, "Acute Stress and an Electrolyte- Imbalanced Diet, but Not Chronic Hypoxia, Increase Oxidative Stress and Hamper Innate Immune Status in a Rainbow Trout (Oncorhynchus mykiss) Isogenic Line", Frontiers in Physiology, 10, pp.453~ (2019), Frontiers Media SA[DOI]
- Ludson Guimarães Manduca, "Effects of different stocking densities on Nile tilapia performance and profitability of a biofloc system with a minimum water exchange", Aquaculture, 530, pp.735814~ (2021), Elsevier BV[DOI]
- Charlene Menezes, "The influence of stocking density and food deprivation in silver catfish (Rhamdia quelen): A metabolic and endocrine approach", Aquaculture, 435, pp.257~264 (2015), Elsevier BV[DOI]
- Antonio Francisco Millán-Cubillo, "Low stocking density negatively affects growth, metabolism and stress pathways in juvenile specimens of meagre (Argyrosomus regius, Asso 1801)", Aquaculture, 451, pp.87~92 (2016), Elsevier BV[DOI]
- A. Möck, "Lysozyme activity in rainbow trout, Oncorhynchus mykiss (Walbaum), stressed by handling, transport and water pollution", Journal of Fish Biology, 37(6), pp.873~885 (1990), Wiley[DOI]
- Thomas P. Mommsen, "Cortisol in teleosts: Dynamics, mechanisms of action, and metabolic regulation", Reviews in Fish Biology and Fisheries, 9(3), pp.211~268 (1999), Springer Science and Business Media LLC[DOI]
- D. Montero, "High stocking density produces crowding stress altering some physiological and biochemical parameters in gilthead seabream, Sparus aurata, juveniles", Fish Physiology and Biochemistry, 20(1), pp.53~60 (1999), Springer Science and Business Media LLC[DOI]
- D. Montero, "Alteration of liver and muscle fatty acid composition in gilthead seabream (Sparus aurata) juveniles held at high stocking density and fed an essential fatty acids deficient diet", Fish Physiology and Biochemistry, 24(1), pp.63~72 (2001), Springer Science and Business Media LLC[DOI]
- Mahdi Naderi, "Effects of chronic high stocking density on liver proteome of rainbow trout (Oncorhynchus mykiss)", Fish Physiology and Biochemistry, 43(5), pp.1373~1385 (2017), Springer Science and Business Media LLC[DOI]
- NIFS(National Institute of Fisheries Science), "Manual for Rainbow Trout Farming", NIFS, ~ (2017)
- Meng Ni, "Effects of stocking density on mortality, growth and physiology of juvenile amur sturgeon (Acipenser schrenckii)", Aquaculture Research, 47(5), pp.1596~1604 (2014), Hindawi Limited[DOI]
- B.P. North, "The impact of stocking density on the welfare of rainbow trout (Oncorhynchus mykiss)", Aquaculture, 255(1-4), pp.466~479 (2006), Elsevier BV[DOI]
- Pickering AD, "Growth and stress in fish production", Proceedings of the Fourth International Symposium on Genetics in Aquaculture, pp.51~63 (1993), Windermere Laboratory
- Yuanyuan Ren, "Effects of stocking density on lipid deposition and expression of lipid-related genes in Amur sturgeon (Acipenser schrenckii)", Fish Physiology and Biochemistry, 43(6), pp.1707~1720 (2017), Springer Science and Business Media LLC[DOI]
- Stuart J. Rowland, "Effects of stocking density on the performance of the Australian freshwater silver perch (Bidyanus bidyanus) in cages", Aquaculture, 253(1-4), pp.301~308 (2006), Elsevier BV[DOI]
- Emilio Salas-Leiton, "Effects of stocking density and feed ration on growth and gene expression in the Senegalese sole (Solea senegalensis): Potential effects on the immune response", Fish Shell Immuno, 28(2), pp.296~302 (2010), Elsevier BV[DOI]
- Narasimhulu Sadhu, "Chronic stress due to high stocking density in open sea cage farming induces variation in biochemical and immunological functions in Asian seabass (Lates calcarifer, Bloch)", Fish Physiology and Biochemistry, 40, pp.1105~1113 (2014), Springer Science and Business Media LLC[DOI]
- Sophie Sammouth, "The effect of density on sea bass (Dicentrarchus labrax) performance in a tank-based recirculating system", Aquacultural Engineering, 40(2), pp.72~78 (2009), Elsevier BV[DOI]
- Shailesh Saurabh, "Lysozyme: an important defence molecule of fish innate immune system", Aquaculture Research, 39(3), pp.223~239 (2008), Hindawi Limited[DOI]
- Ahmad Tahmasebi-Kohyani, "Effects of dietary nucleotides supplementation on rainbow trout (Oncorhynchus mykiss) performance and acute stress response", Fish Physiology and Biochemistry, 38(2), pp.431~440 (2011), Springer Science and Business Media LLC[DOI]
- M TELES, "Endocrine and metabolic responses of Anguilla anguilla L. caged in a freshwater–wetland (Pateira de Fermentelos—Portugal)", Science of The Total Environment, 372(2-3), pp.562~570 (2007), Elsevier BV[DOI]
- Imorou Toko, "Rearing of African catfish (Clarias gariepinus) and vundu catfish (Heterobranchus longifilis) in traditional fish ponds (whedos): Effect of stocking density on growth, production and body composition", Aquaculture, 262(1), pp.65~72 (2007), Elsevier BV[DOI]
- Carlos Eduardo Tolussi, "The effects of stocking density in physiological parameters and growth of the endangered teleost species piabanha, Brycon insignis (Steindachner, 1877)", Aquaculture, 310(1-2), pp.221~228 (2010), Elsevier BV[DOI]
- Cristina E. Trenzado, "Physiological effects of crowding in rainbow trout, Oncorhynchus mykiss, selected for low and high stress responsiveness", Aquaculture, 258(1-4), pp.583~593 (2006), Elsevier BV[DOI]
- L. Vargas-Chacoff, "Combined effects of high stocking density and Piscirickettsia salmonis treatment on the immune system, metabolism and osmoregulatory responses of the Sub-Antarctic Notothenioid fish Eleginops maclovinus", Fish Shellfish Immun, 40(2), pp.424~434 (2014), Elsevier BV[DOI]
- Peyman Yarahmadi, "Increased stocking density causes changes in expression of selected stress- and immune-related genes, humoral innate immune parameters and stress responses of rainbow trout (Oncorhynchus mykiss)", Fish Shellfish Immunol, 48, pp.43~53 (2016), Elsevier BV[DOI]
- Peyman Yarahmadi, "The effects of stocking density on hemato-immunological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss)", Aquaculture International, 23(1), pp.55~63 (2014), Springer Science and Business Media LLC[DOI]
- Z. Yin, "The effects of crowding stress on the non-specific immuneresponse in fancy carp (Cyprinus carpio L.)", Fish Shellfish Immunol, 5(7), pp.519~529 (1995), Elsevier BV[DOI]
- Yoon JM, "Optimal stocking density and environmental factors of rainbow trout(Oncorhynchus mykiss)in semi-closed recirculation system", Korean J Ecol Environ, 28, pp.183~189 (1995)
- Chuanpeng Zhou, "Effect of dietary carbohydrate on non-specific immune response, hepatic antioxidative abilities and disease resistance of juvenile golden pompano (Trachinotus ovatus)", Fish Shellfish Immunol, 41(2), pp.183~190 (2014), Elsevier BV[DOI]
- Xuxia Zhou, "Effect of treatment with probiotics as water additives on tilapia (Oreochromis niloticus) growth performance and immune response", Fish Physiology and Biochemistry, 36(3), pp.501~509 (2010), Springer Science and Business Media LLC[DOI]
- Tengfei Zhu, "Feed intake, growth performance and cholesterol metabolism in juvenile turbot ( Scophthalmus maximus L.) fed defatted fish meal diets with graded levels of cholesterol", Aquaculture, 428-429, pp.290~296 (2014), Elsevier BV[DOI]
