생체 외 및 생체 내 실험을 통한 모무늬돌김(Pyropia seriata) 열수추출물 의 면역 증진 효과
Immune-enhancing Effect of Aqueous Extract of Pyropia seriata In vitro and In vivo
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Pyropia seriata (PS), a seaweed belonging to the red algae family, is mainly cultivated in the coastal areas of Korea and Japan. PS has been reported to have functions related to heat stress tolerance; however, its other functions remain unclear. Therefore, in this study, we investigated the immune-enhancing effects of aqueous extract of PS (AePS) using mouse RAW264.7 macrophages and cyclophosphamide (CPA)-induced immuno-suppressed experimental animals. AePS significantly increased the expression of nitric oxide (NO), inducible nitiric oxide synthase, interleukin-6, and tumor necrosis factor-α without toxicity in RAW264.7 cells. Western blotting and NO assay confirmed that these effects were mediated through the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways. Daily oral administration of AePS to CPA-induced immune-suppressed animals for two weeks restored the size of their spleen to that of normal animals and caused a significant increase in splenocyte proliferation. The results of this study suggest that PS exerts an immune-enhancing effect by inducing the expression of pro-inflammatory cytokines, indicating its protential application as an immune-related functional materials.
Abstract
Pyropia seriata (PS), a seaweed belonging to the red algae family, is mainly cultivated in the coastal areas of Korea and Japan. PS has been reported to have functions related to heat stress tolerance; however, its other functions remain unclear. Therefore, in this study, we investigated the immune-enhancing effects of aqueous extract of PS (AePS) using mouse RAW264.7 macrophages and cyclophosphamide (CPA)-induced immuno-suppressed experimental animals. AePS significantly increased the expression of nitric oxide (NO), inducible nitiric oxide synthase, interleukin-6, and tumor necrosis factor-α without toxicity in RAW264.7 cells. Western blotting and NO assay confirmed that these effects were mediated through the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways. Daily oral administration of AePS to CPA-induced immune-suppressed animals for two weeks restored the size of their spleen to that of normal animals and caused a significant increase in splenocyte proliferation. The results of this study suggest that PS exerts an immune-enhancing effect by inducing the expression of pro-inflammatory cytokines, indicating its protential application as an immune-related functional materials.
참고문헌 (33)
- Ahlmann M, "The effect of cyclophosphamide on the immune system : Implications for clinical cancer therapy", Cancer Chemother Pharmacol, 78, pp.661~671 (2016)[DOI]
- Apetoh L, "Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy", Nat Med, 13, pp.1050~1059 (2007)[DOI]
- Atri C, "Role of human macrophage polarization in inflammation during infectious diseases", Int J Mol Sci, 19, pp.1801~ (2018)[DOI]
- Baek SY, "Comparison of quality characteristics and antioxidant activities between Porphyra yezoensis and Porphyra dentata in Korea", J Korean Soc Food Sci Nutr, 48, pp.1233~1243 (2019)[DOI]
- Cho BM, "Constituents and antioxidant activities of lavers(Pyropia spp. )bred at the southwestern coastal area of Korea", Kor J Food Sci Technol, 53, pp.669~681 (2021)[DOI]
- Choi CW, "Pharmaceutical composition Porphyra dentata Kjellman extract for skin regeneration. KR patent 10-1981428", Korean Intellectual Property Office, ~ (2019)
- Cooper MD, "The evolution of adaptive immune systems", Cell, 124, pp.815~822 (2006)[DOI]
- Drazen DL, "Melatonin enhancement of splenocyte proliferation is attenuated by luzindole, a melatonin receptor antagonist", Am J Physiol RegulIntegr Comp Physiol, 280, pp.R1476~R1482 (2001)[DOI]
- Elmore SA, "Enhanced histopathology of the spleen", Toxicol Pathol, 34, pp.648~655 (2006)[DOI]
- Gourbal B, "Innate immune memory : An evolutionary perspective", Immunol Rev, 283, pp.21~40 (2018)[DOI]
- Han NR, "The immune-enhancing effect of anthocyanin-fucoidan nanocomplex in RAW264. 7 macrophages and cyclophophamide-induced immunosuppressed mice", J Food Biochem, 45, pp.e13631~ (2021)[DOI]
- Hirayama D, "The phagocytic function of macrophage-enforcing innate immunity and tissue homeostasis", Int J Mol Sci, 19, pp.92~ (2017)[DOI]
- Hossain A, "Sulfated polysaccharides in sea cucumbers and their biological properties : A review", Int J Biol Macromol, 253, pp.127329~ (2023)[DOI]
- Jang GE, "Immune enhancing effects of Pyropia yezoensis hydrothermal extract in RAW264. 7 cells", J Mar Biosci Biotechnol, 15, pp.33~40 (2023)[DOI]
- Kahn AA, "Pulling the brakes on fast and furious multiple drug-resistant(MDR)bacteria", Int J Mol Sci, 22, pp.859~ (2021)[DOI]
- Khatua S, "Understanding immune-modulatory efficacy in vitro", Chem Bio Interact, 352, pp.109776~ (2022)[DOI]
- Kim HI, "Immune-enhancing effect of Sargassum horneri on cyclophosphamide-induced immunosuppression in BALB", c mice and primary cultured splenocytes, 2022, pp.27, 8253~
- Kim JH, "Laver production regions recorded in the official geography books of the Joseon dynasty", J Kor Sor Fish Mar Edu, 35, pp.150~160 (2023)[DOI]
- Liu Z, "Anti-cancer activity of porphyran and carrageenan from red seaweeds", Molecules, 24, pp.4286~ (2019)[DOI]
- Luo M, "Antitumor and adjuvant activity of λ-carrageenan by stimulating immune response in cancer immunotherapy", Sci Rep, 5, pp.1~12 (2015)[DOI]
- Mansila MJ, "Hsp70 regulates immune response in experimental autoimmune encephalomyelitis", PLoS One, 9, pp.e105737~ (2014)[DOI]
- Martinex JL, "General principles of antibiotic resistance in bacteria", Drug Discov Today Technol, 11, pp.33~39 (2014)[DOI]
- Martinez FO, "Macrophage activation and polarization", Front Biosci, 13, pp.453~461 (2008)[DOI]
- Monmai C, "Immune-enhancement and anti-inflammatory activities of fatty acids extracted from Halocynthia aurantium tunic in RAW264. 7 cells", Mar Drugs, 16, pp.309~ (2018)[DOI]
- Monmai C, "Transgenic rice seed extracts exert immunomodulatory effects by modulating immune-related biomarkers in RAW264. 7 macrophage cells", Nutrients, 14, pp.4143~ (2022)[DOI]
- Omara FO, "Immunomodulatory and protective effects of N-acetylcysteine in mitogen-activated murine splenocytes in vitro", Toxicology, 116, pp.219~226 (1997)[DOI]
- Park HS, "Heat shock protein gene family of the Porphyra seriata and enhancement of heat stress tolerance by PsHSP70 in Chlamydomonas", Mar Biotechnol, 14, pp.332~342 (2012)[DOI]
- Park WM, "Studies on organic acid, vitamin and free sugar contents of commercial dried lavers(Porphyra yezoensis)cultivated in Korea", J Korean Soc Food Sci Nutr, 43, pp.172~177 (2014)[DOI]
- Pezzanite LM, "Toll-like receptor activation of equine mesenchymal stromal cells to enhance antibacterial activity and immunomodulatory cytokine secretion", Vet Surg, 50, pp.858~871 (2021)[DOI]
- Song JH, "Extracts of Porphyra tenera(Nori seaweed)activate the immune response in mouse RAW264. 7 macrophages via NF-kappaB signaling", J Med Food, 20, pp.1152~1159 (2017)[DOI]
- Sudeep HV, "A standardized extract of Echinacea purpurea containing higher chicoric acid content enhances immune function in murine macrophages and cyclophosphamide-induced immunosuppression mice", Pharm Bio, l61, pp.1211~1221 (2023)[DOI]
- Yatim KM, "A brief journey through the immune system", Clin J Am Soc Nephrol, 10, pp.1274~1281 (2015)[DOI]
- Yu Y, "Biological activities and pharmaceutical applications of polysaccharide from natural resources : A review", Carbohydr Polym, 183, pp.91~101 (2018)[DOI]