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연자육(Lotus Nelumbo nucifera Seed) 단백질로부터 항산화 펩타이드 분리 정제 및 특성

Purification and Characterization of Antioxidant Peptides from Lotus Nelumbo nucifera Seed Protein

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초록

Lotus Nelumbo nucifera seed protein (LSP) was isolated by alkaline solubilization after removing fat and phenolics by hexane and ethanol treatment. Antioxidant peptides from LSP were produced with Alcalase® and pepsin and hydroxyl radical scavenging activities were determined. LSP-Alcalase® hydrolysates showed higher hydroxyl radical scavenging activity than LSP-pepsin hydrolysates. To purify antioxidant peptides, LSP-Alcalase® hydrolysates were subjected to high performance liquid chromatography (HPLC) separation on the C18 column and the active fraction was further purified using a SuperdexTM peptide 10/300 GL column. Finally, the active fraction (F8-2) was evaluated for antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging, and oxygen radical absorbance capacity (ORAC) assays. The EC50 values of the F8-2 were 105.81±0.02 μg/mL for DPPH and 32.26±0.02 μg/mL for hydroxyl radical and the F8-2 exhibited 7.22 μM trolox equivalent (TE)/100 μg F8-2. Glutathione (GSH), which is a positive control, showed EC50 values of 19.87±0.01 μg/mL for DPPH and 15.95±0.03 μg/mL for hydroxyl radical and an ORAC value of 14.17±0.03 μM TE/100 μg GSH. Finally, sixteen peptides were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among them, Ile-Tyr and Leu-Tyr showed higher antioxidant scores.

Abstract

Lotus Nelumbo nucifera seed protein (LSP) was isolated by alkaline solubilization after removing fat and phenolics by hexane and ethanol treatment. Antioxidant peptides from LSP were produced with Alcalase® and pepsin and hydroxyl radical scavenging activities were determined. LSP-Alcalase® hydrolysates showed higher hydroxyl radical scavenging activity than LSP-pepsin hydrolysates. To purify antioxidant peptides, LSP-Alcalase® hydrolysates were subjected to high performance liquid chromatography (HPLC) separation on the C18 column and the active fraction was further purified using a SuperdexTM peptide 10/300 GL column. Finally, the active fraction (F8-2) was evaluated for antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging, and oxygen radical absorbance capacity (ORAC) assays. The EC50 values of the F8-2 were 105.81±0.02 μg/mL for DPPH and 32.26±0.02 μg/mL for hydroxyl radical and the F8-2 exhibited 7.22 μM trolox equivalent (TE)/100 μg F8-2. Glutathione (GSH), which is a positive control, showed EC50 values of 19.87±0.01 μg/mL for DPPH and 15.95±0.03 μg/mL for hydroxyl radical and an ORAC value of 14.17±0.03 μM TE/100 μg GSH. Finally, sixteen peptides were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among them, Ile-Tyr and Leu-Tyr showed higher antioxidant scores.

참고문헌 (29)

  1. Habtamu Admassu, "Bioactive Peptides Derived from Seaweed Protein and Their Health Benefits: Antihypertensive, Antioxidant, and Antidiabetic Properties", Journal of Food Science, 83(1), pp.6~16 (2017), Wiley[DOI]
  2. Mohsen Akbarian, "Bioactive Peptides: Synthesis, Sources, Applications, and Proposed Mechanisms of Action", International Journal of Molecular Sciences, 23(3), pp.1445~ (2022), MDPI AG[DOI]
  3. Sneh Punia Bangar, "A comprehensive review on lotus seeds (Nelumbo nucifera Gaertn.): Nutritional composition, health-related bioactive properties, and industrial applications", Journal of Functional Foods, 89, pp.104937~ (2022), Elsevier BV[DOI]
  4. Maiza Lacerda Barbosa, "Oxidative stress, antioxidant defense and depressive disorders: A systematic review of biochemical and molecular markers", Neurology, Psychiatry and Brain Research, 36, pp.65~72 (2020), Elsevier BV[DOI]
  5. Won-Kyung Cho, "Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin", Journal of Functional Foods, 91, pp.105019~ (2022), Elsevier BV[DOI]
  6. Valérie Conway, "Antioxidant Activities of Buttermilk Proteins, Whey Proteins, and Their Enzymatic Hydrolysates", Journal of Agricultural and Food Chemistry, 61(2), pp.364~372 (2012), American Chemical Society (ACS)[DOI]
  7. Lili Hao, "A comprehensive review of oyster peptides: Preparation, characterisation and bioactivities", Reviews in Aquaculture, 14(1), pp.120~138 (2021), Wiley[DOI]
  8. Sam Sun Kim, "Purification and antioxidant activities of peptides from sea squirt (Halocynthia roretzi) protein hydrolysates using pepsin hydrolysis", Food Bioscience, 25, pp.128~133 (2018), Elsevier BV[DOI]
  9. Young-Sang Kim, "Anti-inflammatory action of high molecular weight Mytilus edulis hydrolysates fraction in LPS-induced RAW264.7 macrophage via NF-κB and MAPK pathways", Food Chemistry, 202, pp.9~14 (2016), Elsevier BV[DOI]
  10. Yi Lei, "Structural characterization and in vitro analysis of the prebiotic activity of oligosaccharides from lotus (Nelumbo nucifera Gaertn.) seeds", Food Chemistry, 388, pp.133045~ (2022), Elsevier BV[DOI]
  11. Xican Li, "Solvent effects and improvements in the deoxyribose degradation assay for hydroxyl radical-scavenging", Food Chemistry, 141(3), pp.2083~2088 (2013), Elsevier BV[DOI]
  12. Wen-Ying Liu, "Antioxidant properties and inhibition of angiotensin-converting enzyme by highly active peptides from wheat gluten", Scientific Reports, 11(1), pp.5206~ (2021), Springer Science and Business Media LLC[DOI]
  13. Jose M. Lorenzo, "Bioactive peptides as natural antioxidants in food products – A review", Trends Food Sci Technol, 79, pp.136~147 (2018), Elsevier BV[DOI]
  14. Chathuri Kaushalya Marasinghe, "Anti-inflammatory action of ark shell (Scapharca subcrenata) protein hydrolysate in LPS-stimulated RAW264.7 murine macrophages", Journal of Food Biochemistry, 46(12), pp.e14493~ (2022), Hindawi Limited[DOI]
  15. Anwar Noman, "Fractionation and purification of antioxidant peptides from Chinese sturgeon (Acipenser sinensis) protein hydrolysates prepared using papain and alcalase 2.4L", Arabian Journal of Chemistry, 15(12), pp.104368~ (2022), Elsevier BV[DOI]
  16. Yunok Oh, "Amino Acid Composition, Antioxidant, and Cytoprotective Effect of Blue Mussel (Mytilus edulis) Hydrolysate through the Inhibition of Caspase-3 Activation in Oxidative Stress-Mediated Endothelial Cell Injury", Marine Drugs, 17(2), pp.135~ (2019), MDPI AG[DOI]
  17. Yunok Oh, "Blue Mussel-Derived Peptides PIISVYWK and FSVVPSPK Trigger Wnt", β-Catenin Signaling-Mediated Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells, 2020, pp.18(10), 510~[DOI]
  18. Bahareh H. Sarmadi, "Antioxidative peptides from food proteins: A review", Peptides, 31(10), pp.1949~1956 (2010), Elsevier BV[DOI]
  19. 신희재, "연자육과 연자방 추출물을 이용한 세포 내 미백 효능 유효성 평가", 아시안뷰티화장품학술지, 20(1), pp.111~120 (2022), 한국피부과학연구원[DOI]
  20. Song CC, "Study on the domain selective inhibition of angiotensin-converting enzyme (ACE) by food-derived tyrosin-containing dipeptides", J Food Biochem, 45, pp.e13779~ (2020)[DOI]
  21. Solomon Abebaw Tadesse, "Production and processing of antioxidant bioactive peptides: A driving force for the functional food market", Heliyon, 6(8), pp.e04765~ (2020), Elsevier BV[DOI]
  22. Chibuike C. Udenigwe, "Chemometric Analysis of the Amino Acid Requirements of Antioxidant Food Protein Hydrolysates", International Journal of Molecular Sciences, 12(5), pp.3148~3161 (2011), MDPI AG[DOI]
  23. Chaoting Wen, "Plant protein-derived antioxidant peptides: Isolation, identification, mechanism of action and application in food systems: A review", Trends Food Sci Technol, 105, pp.308~322 (2020), Elsevier BV[DOI]
  24. Naijin Xu, "Antioxidative Categorization of Twenty Amino Acids Based on Experimental Evaluation", Molecules, 22(12), pp.2066~ (2017), MDPI AG[DOI]
  25. YueTong Yu, "Identification and quantification of oligomeric proanthocyanidins, alkaloids, and flavonoids in lotus seeds: A potentially rich source of bioactive compounds", Food Chemistry, 379, pp.132124~ (2022), Elsevier BV[DOI]
  26. Ahmed A. Zaky, "Bioactivities, Applications, Safety, and Health Benefits of Bioactive Peptides From Food and By-Products: A Review", Frontiers in Nutrition, 8, pp.815640~ (2022), Frontiers Media SA[DOI]
  27. Hong‐Yan Zeng, "Amino acid profiles and quality from lotus seed proteins", Journal of the Science of Food and Agriculture, 93(5), pp.1070~1075 (2012), Wiley[DOI]
  28. Tongxin Zhi, "Novel antioxidant peptides from protein hydrolysates of scallop (Argopecten irradians) mantle using enzymatic and microbial methods: Preparation, purification, identification and characterization", LWT, 164, pp.113636~ (2022), Elsevier BV[DOI]
  29. Ana Zulueta, "ORAC and TEAC assays comparison to measure the antioxidant capacity of food products", Food Chemistry, 114(1), pp.310~316 (2009), Elsevier BV[DOI]

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