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In vitro and in vivo anti-inflammatory activities of a sterol-enriched fraction from freshwater green alga, Spirogyra sp.

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Background: Inflammation plays a crucial role in the pathogenesis of many diseases such as arthritis and atherosclerosis. In the present study, we evaluated anti-inflammatory activity of sterol-rich fraction prepared from Spirogyra sp., a freshwater green alga, in an effort to find bioactive extracts derived from natural sources. Methods: The sterol content of ethanol extract of Spirogyra sp. (SPE) was enriched by fractionation with hexane (SPEH), resulting 6.7 times higher than SPE. Using this fraction, the in vitro and in vivo anti-inflammatory activities were evaluated in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells and zebrafish. Results: SPEH effectively and dose-dependently decreased the production of nitric oxide (NO) and prostaglandin E2 (PGE2). SPEH suppressed the production of pro-inflammatory cytokines including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and IL-1β through downregulating nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW 264.7 cells without cytotoxicity. The in vivo test results indicated that SPEH significantly and dose-dependently reduced reactive oxygen species (ROS) generation, cell death, and NO production in LPS-stimulated zebrafish. Conclusions: These results demonstrate that SPEH possesses strong in vitro and in vivo anti-inflammatory activities and has the potential to be used as healthcare or pharmaceutical material for the treatment of inflammatory diseases.

Abstract

Background: Inflammation plays a crucial role in the pathogenesis of many diseases such as arthritis and atherosclerosis. In the present study, we evaluated anti-inflammatory activity of sterol-rich fraction prepared from Spirogyra sp., a freshwater green alga, in an effort to find bioactive extracts derived from natural sources. Methods: The sterol content of ethanol extract of Spirogyra sp. (SPE) was enriched by fractionation with hexane (SPEH), resulting 6.7 times higher than SPE. Using this fraction, the in vitro and in vivo anti-inflammatory activities were evaluated in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells and zebrafish. Results: SPEH effectively and dose-dependently decreased the production of nitric oxide (NO) and prostaglandin E2 (PGE2). SPEH suppressed the production of pro-inflammatory cytokines including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and IL-1β through downregulating nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW 264.7 cells without cytotoxicity. The in vivo test results indicated that SPEH significantly and dose-dependently reduced reactive oxygen species (ROS) generation, cell death, and NO production in LPS-stimulated zebrafish. Conclusions: These results demonstrate that SPEH possesses strong in vitro and in vivo anti-inflammatory activities and has the potential to be used as healthcare or pharmaceutical material for the treatment of inflammatory diseases.

참고문헌 (37)

  1. Yasantha Athukorala, "Screening for Angiotensin 1-Converting Enzyme Inhibitory Activity of Ecklonia cava", Preventive Nutrition and Food Science, 10(2), pp.134~139 (2005), The Korean Society of Food Science and Nutrition[DOI]
  2. Abdulrahman M. Elbagory, "New polyhydroxylated sterols from Palythoa tuberculosa and their apoptotic activity in cancer cells", Steroids, 101, pp.110~115 (2015), Elsevier BV[DOI]
  3. I. P. Shanura Fernando, "Inhibition of inflammatory responses elicited by urban fine dust particles in keratinocytes and macrophages by diphlorethohydroxycarmalol isolated from a brown alga Ishige okamurae", ALGAE, 32(3), pp.261~273 (2017), 한국조류학회I[DOI]
  4. I.P. Shanura Fernando, "FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae", ALGAE, 32(1), pp.75~86 (2017), 한국조류학회I[DOI]
  5. Gary S. Firestein, "Inhibiting Inflammation in Rheumatoid Arthritis", New England Journal of Medicine, 354(1), pp.80~82 (2006), Massachusetts Medical Society[DOI]
  6. Dulce Maria González Mosquera, "Evaluation of the in vivo anti-inflammatory activity of a flavonoid glycoside from Boldoa purpurascens", Phytochemistry Letters, 4(3), pp.231~234 (2011), Elsevier BV[DOI]
  7. S HEO, "Antioxidant activities of enzymatic extracts from brown seaweeds", Bioresource Technology, 96(14), pp.1613~1623 (2005), Elsevier BV[DOI]
  8. Soo-Jin Heo, "Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages", Food and Chemical Toxicology, 48(8-9), pp.2045~2051 (2010), Elsevier BV[DOI]
  9. Won-Kyo Jung, "Anti-inflammatory activity of caffeic acid phenethyl ester (CAPE) extracted from Rhodiola sacra against lipopolysaccharide-induced inflammatory responses in mice", Process Biochemistry, 43(7), pp.783~787 (2008), Elsevier BV[DOI]
  10. Nalae Kang, "Gallic acid isolated from Spirogyra sp. improves cardiovascular disease through a vasorelaxant and antihypertensive effect", Environmental Toxicology and Pharmacology, 39(2), pp.764~772 (2015), Elsevier BV[DOI]
  11. Kazłowska K, "In vitro and in vivo anticancer effects of sterol fraction from red algae Porphyra dentata", Evid Based Complement Alternat Med, 2013, pp.493869~ (2013)[DOI]
  12. M KHALAF, "Biosorption of reactive dye from textile wastewater by non-viable biomass of Aspergillus niger and Spirogyra sp.", Bioresource Technology, 99(14), pp.6631~6634 (2008), Elsevier BV[DOI]
  13. 김현호, "A single-step isolation of useful antioxidant compounds from Ishige okamurae by using centrifugal partition chromatography", Fisheries and Aquatic Sciences, 19(2), pp.1~7 (2016), 한국수산과학회
  14. S. F. Kim, "Inducible Nitric Oxide Synthase Binds, S-Nitrosylates, and Activates Cyclooxygenase-2", Science, 310(5756), pp.1966~1970 (2005), American Association for the Advancement of Science (AAAS)[DOI]
  15. Shuji Kishi, "The zebrafish as a vertebrate model of functional aging and very gradual senescence", Experimental Gerontology, 38(7), pp.777~786 (2003), Elsevier BV[DOI]
  16. Andis Klegeris, "Therapeutic approaches to inflammation in neurodegenerative disease", Current Opinion in Neurology, 20(3), pp.351~357 (2007), Ovid Technologies (Wolters Kluwer Health)[DOI]
  17. Seok-Chun Ko, "Protective effect of Ecklonia cava on UVB-induced oxidative stress: in vitro and in vivo zebrafish model", Journal of Applied Phycology, 23(4), pp.697~708 (2011), Springer Science and Business Media LLC[DOI]
  18. Eiichi Kotake-Nara, "Neoxanthin and fucoxanthin induce apoptosis in PC-3 human prostate cancer cells", Cancer Letters, 220(1), pp.75~84 (2005), Elsevier BV[DOI]
  19. Chai Siah Ku, "Edible blue-green algae reduce the production of pro-inflammatory cytokines by inhibiting NF-κB pathway in macrophages and splenocytes", Biochimica et Biophysica Acta (BBA) - General Subjects, 1830(4), pp.2981~2988 (2013), Elsevier BV[DOI]
  20. David M Lee, "Rheumatoid arthritis", The Lancet, 358(9285), pp.903~911 (2001), Elsevier BV[DOI]
  21. Jin-Ching Lee, "Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties", Cancer Cell International, 13(1), pp.55~ (2013), Springer Science and Business Media LLC[DOI]
  22. Ji-Hyeok Lee, "Protective effect of a freshwater alga, Spirogyra sp., against lipid peroxidation in vivo zebrafish and purification of antioxidative compounds using preparative centrifugal partition chromatography", Journal of Applied Phycology, 28(1), pp.181~189 (2016), Springer Science and Business Media LLC[DOI]
  23. Seung-Hong Lee, "Molecular characteristics and anti-inflammatory activity of the fucoidan extracted from Ecklonia cava", Carbohydrate Polymers, 89(2), pp.599~606 (2012), Elsevier BV[DOI]
  24. Birhanetensay Masresha, "In vivo anti-inflammatory activities of Ocimum suave in mice", Journal of Ethnopharmacology, 142(1), pp.201~205 (2012), Elsevier BV[DOI]
  25. Alejandro M. S. Mayer, "Marine Pharmacology in 2000: Marine Compounds with Antibacterial, Anticoagulant, Antifungal, Anti-inflammatory, Antimalarial, Antiplatelet, Antituberculosis, and Antiviral Activities; Affecting the Cardiovascular, Immune, and Nervous Systems and Other Miscellaneous Mechanisms of Action", Marine Biotechnology, 6(1), pp.37~52 (2004), Springer Science and Business Media LLC[DOI]
  26. 오재영, "Potential applications of radioprotective phytochemicals from marine algae", ALGAE, 31(4), pp.403~414 (2016), 한국조류학회I[DOI]
  27. K.K. Asanka Sanjeewa, "In vitro and in vivo anti-inflammatory activities of high molecular weight sulfated polysaccharide; containing fucose separated from Sargassum horneri: Short communication", International Journal of Biological Macromolecules, 107, pp.803~807 (2018), Elsevier BV[DOI]
  28. Kalu Kapuge Asanka Sanjeewa, "Anti-inflammatory and anti-cancer activities of sterol rich fraction of cultured marine microalga Nannochloropsis oculata", ALGAE, 31(3), pp.277~287 (2016), 한국조류학회I[DOI]
  29. Sung-Suk Suh, "Anti-inflammation and Anti-Cancer Activity of Ethanol Extract of Antarctic Freshwater Microalga, Micractinium sp.", International Journal of Medical Sciences, 15(9), pp.929~936 (2018), Ivyspring International Publisher[DOI]
  30. Lei Wang, "Protective effect of polysaccharides from Celluclast-assisted extract of Hizikia fusiforme against hydrogen peroxide-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish", International Journal of Biological Macromolecules, 112, pp.483~489 (2018), Elsevier BV[DOI]
  31. Lei Wang, "Protective effect of gallic acid derivatives from the freshwater green alga Spirogyra sp. against ultraviolet B-induced apoptosis through reactive oxygen species clearance in human keratinocytes and zebrafish", ALGAE, 32(4), pp.379~388 (2017), 한국조류학회I[DOI]
  32. Zheng-Shun Wen, "Composition and anti-inflammatory effect of polysaccharides from Sargassum horneri in RAW264.7 macrophages", International Journal of Biological Macromolecules, 88, pp.403~413 (2016), Elsevier BV[DOI]
  33. W. A. J. P. Wijesinghe, "Anti-inflammatory activity of phlorotannin-rich fermented Ecklonia cava processing by-product extract in lipopolysaccharide-stimulated RAW 264.7 macrophages", Journal of Applied Phycology, 25(4), pp.1207~1213 (2013), Springer Science and Business Media LLC[DOI]
  34. W.A.J.P. Wijesinghe, "5β-Hydroxypalisadin B isolated from red alga Laurencia snackeyi attenuates inflammatory response in lipopolysaccharide-stimulated RAW 264.7 macrophages", ALGAE, 29(4), pp.333~341 (2014), The Korean Society of Phycology[DOI]
  35. W.A.J.P. Wijesinghe, "Assessment of anti-inflammatory effect of 5β-hydroxypalisadin B isolated from red seaweed Laurencia snackeyi in zebrafish embryo in vivo model", Environmental Toxicology and Pharmacology, 37(1), pp.110~117 (2014), Elsevier BV[DOI]
  36. Huanzhang Xiong, "Effects of taraxasterol on iNOS and COX-2 expression in LPS-induced RAW 264.7 macrophages", Journal of Ethnopharmacology, 155(1), pp.753~757 (2014), Elsevier BV[DOI]
  37. Quanbo Xiong, "The Liebermann–Burchard Reaction: Sulfonation, Desaturation, and Rearrangment of Cholesterol in Acid", Lipids, 42(1), pp.87~96 (2007), Wiley[DOI]

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