본문 바로가기
KERD
논문KCI 등재

Preventive effects of sea cucumber (Apostichopus japonicus) ethanol extract on palmitate-induced vascular injury in vivo

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류수산학

초록

Cardiovascular diseases (CVDs) have posed serious public health problems, accounting for nearly 30% of mortality worldwide and their incidence is still increasing. Therefore, new treatment resources are necessary to prevent or manage the ever-increasing population of patients with CVDs. Sea cucumber is well known for its medical and health benefit effects, but it is not well known what/how effect it has on vascular disease. In the present study, we examined the protect effect of sea cucumber, Apostichopus japonicus 80% ethanol extract (AJE) on zebrafish embryo with the stimulation of free fatty acid, palmitate (PA). In vivo study showed that AJE can attenuate PA-induced toxicity through relieving the rapid heartbeat, increasing the survival rate and reducing the malformation in both wild type and Tg (fli1a:eGFP) transgenic zebrafish lines. Additionally, compare with PA treated embryos, the yolk sac area, body length, axial vascular segment (AVS) and intersegmental vessel (ISV) of the co-treatment group of AJE and PA were comparable to the control group. Moreover, AJE lowered the expression of inducible nitric oxide synthase (iNOS), nitric oxide (NO) and inflammation-related genes induced by PA, and inhibited PA-induced vascular development disorders. Our data preliminarily verify that AJE could be a candidate resource for the prevention or therapy of CVDs.

Abstract

Cardiovascular diseases (CVDs) have posed serious public health problems, accounting for nearly 30% of mortality worldwide and their incidence is still increasing. Therefore, new treatment resources are necessary to prevent or manage the ever-increasing population of patients with CVDs. Sea cucumber is well known for its medical and health benefit effects, but it is not well known what/how effect it has on vascular disease. In the present study, we examined the protect effect of sea cucumber, Apostichopus japonicus 80% ethanol extract (AJE) on zebrafish embryo with the stimulation of free fatty acid, palmitate (PA). In vivo study showed that AJE can attenuate PA-induced toxicity through relieving the rapid heartbeat, increasing the survival rate and reducing the malformation in both wild type and Tg (fli1a:eGFP) transgenic zebrafish lines. Additionally, compare with PA treated embryos, the yolk sac area, body length, axial vascular segment (AVS) and intersegmental vessel (ISV) of the co-treatment group of AJE and PA were comparable to the control group. Moreover, AJE lowered the expression of inducible nitric oxide synthase (iNOS), nitric oxide (NO) and inflammation-related genes induced by PA, and inhibited PA-induced vascular development disorders. Our data preliminarily verify that AJE could be a candidate resource for the prevention or therapy of CVDs.

참고문헌 (53)

  1. Aires RD, "Paraquat poisoning induces TNF-α-dependent iNOS", NO mediated hyporesponsiveness of the aorta to vasoconstrictors in rats, 2013, pp.8, e73562~
  2. Besedina A, "NO-synthase activity in patients with coronary heart disease associated with hypertension of different age groups", J Med Biochem, 35, pp.43~49 (2016)
  3. Carter AM, "Complement activation : an emerging player in the pathogenesis of cardiovascular disease", Scientifica, 2012, pp.402783~ (2012)
  4. Chen S, "Sequence determination and anticoagulant and antithrombotic activities of a novel sulfated fucan isolated from the sea cucumber Isostichopus badionotus", Biochim Biophys Acta Gen Subj, 1820, pp.989~1000 (2012)
  5. Csiszar A, "Aging-induced phenotypic changes and oxidative stress impair coronary arteriolar function", Circ Res, 90, pp.1159~1166 (2002)
  6. D’Oria R, "The role of oxidative stress in cardiac disease : from physiological response to injury factor", Oxid Med Cell Longev, 2020, pp.5732956~ (2020)
  7. Ebbesson SOE, "Fatty acids linked to cardiovascular mortality are associated with risk factors", Int J Circumpolar Health, 74, pp.28055~ (2015)
  8. Ganta VC, "LMO2(LIM domain only 2)and endothelial cell migration in developmental and postnatal angiogenesis", Arterioscler Thromb Vasc Biol, 37, pp.1806~1808 (2017)
  9. Giudicessi JR, "Cardiovascular safety of prokinetic agents : a focus on drug-induced arrhythmias", Neurogastroenterol Motil, 30, pp.e13302~ (2018)
  10. Hossain A, "Northern sea cucumber(Cucumaria frondosa) : a potential candidate for functional food, nutraceutical, and pharmaceutical sector", Mar Drugs, 18, pp.274~ (2020)
  11. Hu S, "Hpyerglycemic effect of a mixture of sea cucumber and Cordyceps sinensis in streptozotocin-induced diabetic rat", J Ocean Univ China, 13, pp.271~277 (2013)
  12. Kaptoge S, "World Health Organization cardiovascular disease risk charts : revised models to estimate risk in 21global regions", Lancet Glob Health, 7, pp.E1332~E1345 (2019)
  13. Kareh M, "Anti-proliferative and anti-inflammatory activities of the sea cucumber Holothuria polii aqueous extract", SAGE Open Med, 6, pp.2050312118809541~ (2018)
  14. Kim NY, "Anti-skin cancer activities of Apostichopus japonicus extracts from low-temperature ultrasonification process", J Healthc Eng, 2017, pp.6504890~ (2017)
  15. Lawson ND, "In vivo imaging of embryonic vascular development using transgenic zebrafish", Dev Biol, 248, pp.307~318 (2002)
  16. Lee JL, "Biologics and cardiovascular events in inflammatory arthritis : a prospective national cohort study", Arthritis Res Ther, 20, pp.171~ (2018)
  17. Li S, "VEGFR tyrosine kinase inhibitor II(VRI)induced vascular insufficiency in zebrafish as a model for studying vascular toxicity and vascular preservation", Toxicol Appl Pharmacol, 280, pp.408~420 (2014)
  18. Li Y, "The ets transcription factor Fli-1 in development, cancer and disease", Oncogene, 34, pp.2022~2031 (2015)
  19. Liu F, "Fli1 acts at the top of the transcriptional network driving blood and endothelial development", Curr Biol, 18, pp.P1234~40 (2008)
  20. Liu H, "Early detection system of vascular disease and its application prospect", BioMed Res Int, 2016, pp.1723485~ (2016)
  21. Liu HH, "Hypolipidemic effect of glycosaminoglycans from the sea cucumber Metriatyla scabra in rats fed a cholesterol-supplemented diet", J Agric Food Chem, 50, pp.3602~3606 (2002)
  22. Liu X, "Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus", Carbohydr Polym, 90, pp.1664~1670 (2012)
  23. Mansour MB, "Primary structure and anticoagulant activity of fucoidan from the sea cucumber Holothuria polii", Int J Biol Macromol, 121, pp.1145~1153 (2019)
  24. Matrone G, "Lmo2(LIM-domain-only 2)modulates Sphk1(sphingosine kinase)and promotes endothelial cell migration", Arterioscler Thromb Vasc Biol, 37, pp.1860~1868 (2017)
  25. McCormack MP, "The Lmo2 oncogene initiates leukemia in mice by inducing thymocyte self-renewal", Science, 327, pp.879~883 (2010)
  26. Meng S, "LIM domain only 2 regulates endothelial proliferation, angiogenesis, and tissue regeneration", J Am Heart Assoc, 5, pp.e004117~ (2016)
  27. Miller V, "Fruit, vegetable, and legume intake, and cardiovascular disease and deaths in 18 countries(PURE) : a prospective cohort study", Lancet, 390, pp.2037~2049 (2017)
  28. Monaco C, "Anti-TNF therapy : past, present and future", Int Immunol, 27, pp.55~62 (2015)
  29. Patan S, "Vasculogenesis and angiogenesis as mechanisms of vascular network formation, growth and remodeling", J Neurooncol, 50, pp.1~15 (2000)
  30. Patterson LJ, "Scl is required for dorsal aorta as well as blood formation in zebrafish embryos", Blood, 105, pp.3502~3511 (2005)
  31. Santhanam L, "Inducible NO synthase–dependent S-nitrosylation and activation of arginase1 contribute to age-related endothelial dysfunction", Circ Res, 101, pp.692~702 (2007)
  32. Sarmah S, "Zebrafish as a vertebrate model system to evaluate effects of environmental toxicants on cardiac development and function", Int J Mol Sci, 17, pp.2123~ (2016)
  33. Seo D, "Gene expression analysis of cardiovascular diseases : novel insights into biology and clinical applications", J Am Coll Cardiol, 48, pp.227~235 (2006)
  34. Sinha S, "Fatty acid-induced insulin resistance in L6 myotubes is prevented by inhibition of activation and nuclear localization of nuclear factor kappa B", J Biol Chem, 279, pp.41294~41301 (2004)
  35. Stoclet JC, "Overproduction of nitric oxide in pathophysiology of blood vessels", Biochemistry(Mosc), 63, pp.826~832 (1998)
  36. Sun HJ, "Role of endothelial dysfunction in cardiovascular diseases : the link between inflammation and hydrogen sulfide", Front Pharmacol, 10, pp.1568~ (2020)
  37. Tang GY, "Effects of vegetables on cardiovascular diseases and related mechanisms", Nutrients, 9, pp.857~ (2017)
  38. Thomé MP, "Ratiometric analysis of acridine orange staining in the study of acidic organelles and autophagy", J Cell Sci, 129, pp.4622~4632 (2016)
  39. Tufail T, "Marine bioactives: potentials to reduce the incidence of cardiovascular disorders", Curr Top Nutraceutical Res, 16 (2018)
  40. Umar S, "Nitric oxide and nitric oxide synthase isoforms in the normal, hypertrophic, and failing heart", Mol Cell Biochem, 333, pp.191~201 (2010)
  41. Volpe CMO, "The production of nitric oxide, IL-6, and TNF-alpha in palmitate-stimulated PBMNCs is enhanced through hyperglycemia in diabetes", Oxid Med Cell Longev, 2014, pp.479587~ (2014)
  42. Wang Z, "Antifungal nortriterpene and triterpene glycosides from the sea cucumber Apostichopus japonicus Selenka", Food Chem, 132, pp.295~300 (2012)
  43. World Health Organization, "Prevention of cardiovascular disease : guidelines for assessment and management of total cardiovascular risk" (2007), World Health Organization
  44. Yang J, "Depolymerized glycosaminoglycan and its anticoagulant activities from sea cucumber Apostichopus japonicus", Int J Biol Macromol, 72, pp.699~705 (2015)
  45. Yang J, "Novel branch patterns and anticoagulant activity of glycosaminoglycan from sea cucumber Apostichopus japonicus", Int J Biol Macromol, 72, pp.911~918 (2015)
  46. Yuan S, "Effects of tumour necrosis factor on cardiovascular disease and cancer : a two-sample Mendelian randomization study", eBioMedicine, 59, pp.102956~ (2020)
  47. Zhang H, "Circulating pro-angiogenic micro-ribonucleic acid in patients with coronary heart disease", Interact Cardiovasc Thorac Surg, 27, pp.336~342 (2018)
  48. Zhang H, "Role of TNF-α in vascular dysfunction", Clin Sci, 116, pp.219~230 (2009)
  49. Zhang J, "Palmitate impairs angiogenesis via suppression of cathepsin activity", Mol Med Rep, 15, pp.3644~3650 (2017)
  50. Zhi Y, "Involvement of the nuclear factor-κB signaling pathway in the regulation of CXC chemokine receptor-4 expression in neuroblastoma cells induced by tumor necrosis factor-α", Int J Mol Med, 35, pp.349~357 (2015)
  51. Zhou DD, "Antioxidant food components for the prevention and treatment of cardiovascular diseases : effects, mechanisms, and clinical studies", Oxid Med Cell Longev, 2021, pp.6627355~ (2021)
  52. Zhu H, "Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish", Dev Biol, 281, pp.256~269 (2005)
  53. Ziff OJ, "Digoxin : the good and the bad", Trends Cardiovasc Med, 26, pp.585~595 (2016)

관련 논문AI추천

이 간행물의 다른 논문 (4건)