해양 폐부표에서 분리한 Flavobacterium sp. BS7의 폴리스티렌 생분해 특성
Polystyrene Biodegradation Characteristics of Flavobacterium sp. BS7 Isolated from a Discarded Marine Buoy
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Marine plastic pollution poses a critical threat to aquatic ecosystems, and polystyrene (PS) is particularly persistent because of its hydrophobicity and resistance to microbial degradation. In this study, we isolated and characterized a PS-degrading bacterium from a discarded marine buoy collected near the coast of Cheongsapo, Busan, Republic of Korea. The isolate, designated as strain BS7, was identified as Flavobacterium sp. on the basis of 16S rRNA gene sequencing. Biodegradation experiments were conducted in a carbon-free basal medium supplemented with PS film (1.5×1.5 cm), and incubation was performed for 30 days at 30°C. The PS films exposed to BS7 showed a significant weight loss of 2.01±0.01% when compared with the control group. Scanning electron microscopy revealed surface erosion, cracking, and biofilm formation on the PS films treated with BS7. Water contact angle analysis revealed a decrease from 74.3° to 64.2°, indicating enhanced surface hydrophilicity. These findings suggested that Flavobacterium sp. BS7 has the potential to initiate polystyrene biodegradation and highlighted its applicability in marine plastic waste mitigation strategies through microbial bioremediation.
Abstract
Marine plastic pollution poses a critical threat to aquatic ecosystems, and polystyrene (PS) is particularly persistent because of its hydrophobicity and resistance to microbial degradation. In this study, we isolated and characterized a PS-degrading bacterium from a discarded marine buoy collected near the coast of Cheongsapo, Busan, Republic of Korea. The isolate, designated as strain BS7, was identified as Flavobacterium sp. on the basis of 16S rRNA gene sequencing. Biodegradation experiments were conducted in a carbon-free basal medium supplemented with PS film (1.5×1.5 cm), and incubation was performed for 30 days at 30°C. The PS films exposed to BS7 showed a significant weight loss of 2.01±0.01% when compared with the control group. Scanning electron microscopy revealed surface erosion, cracking, and biofilm formation on the PS films treated with BS7. Water contact angle analysis revealed a decrease from 74.3° to 64.2°, indicating enhanced surface hydrophilicity. These findings suggested that Flavobacterium sp. BS7 has the potential to initiate polystyrene biodegradation and highlighted its applicability in marine plastic waste mitigation strategies through microbial bioremediation.