Bacterial community comparison revealed by metagenomic analysis and physicochemical properties of eastern little tuna (Euthynnus affinis) with storage temperature differences
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Post-harvest handling and hygienic level of aquatic products significantly affect the quality and level of safety. Cold chain control is one of the determining factors for the quality of fish and the bacterial community that grows on the fish. Identification of spoilage bacteria and pathogens in aquatic products must be made because it will determine the physical and chemical quality. A molecular identification method with high sensitivity is the solution. This study aims to identify the quality of fish and bacterial communities that grow. The research procedures included sample collection, pH measurement, drip loss measurement, transportation and cold storage treatment, DNA extraction, DNA sequencing, sequence analysis, and bioinformatics analysis. The conclusion obtained from this study is that the simulation of the cold chain system applied to eastern little tuna does not significantly affect changes in the water activity value, pH, and drip loss. The insignificant change indicates that the eastern little tuna samples are still in good quality. The bioinformatics analysis showed the highest diversity and abundance of the bacterial community came from the Gammaproteobacterial class.
Abstract
Post-harvest handling and hygienic level of aquatic products significantly affect the quality and level of safety. Cold chain control is one of the determining factors for the quality of fish and the bacterial community that grows on the fish. Identification of spoilage bacteria and pathogens in aquatic products must be made because it will determine the physical and chemical quality. A molecular identification method with high sensitivity is the solution. This study aims to identify the quality of fish and bacterial communities that grow. The research procedures included sample collection, pH measurement, drip loss measurement, transportation and cold storage treatment, DNA extraction, DNA sequencing, sequence analysis, and bioinformatics analysis. The conclusion obtained from this study is that the simulation of the cold chain system applied to eastern little tuna does not significantly affect changes in the water activity value, pH, and drip loss. The insignificant change indicates that the eastern little tuna samples are still in good quality. The bioinformatics analysis showed the highest diversity and abundance of the bacterial community came from the Gammaproteobacterial class.
참고문헌 (42)
- Andersen KS, "Ampvis2: an R package to analyse and visualise 16S rRNA amplicon data" (2018)
- Arnold SH, "Histamine formation by bacteria isolated from skipjack tuna, Katsuwonus pelamis", Bull Jpn Soc Sci Fish, 46, pp.991~995 (1980)
- Bokulich NA, "Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2’s q2-feature-classifier plugin", Microbiome, 6, pp.90~ (2018)
- Bolyen E, "Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2", Nat Biotechnol, 37, pp.852~857 (2019)
- Callahan BJ, "DADA2 : high-resolution sample inference from illumina amplicon data", Nat Methods, 13, pp.581~583 (2016)
- Carda-Diéguez M, "Pyrosequencing survey of intestinal microbiota diversity in cultured sea bass(Dicentrarchus labrax)fed functional diets", FEMS Microbiol Ecol, 87, pp.451~459 (2014)
- Drzewiecka D, "Significance and roles of Proteus spp. bacteria in natural environments", Microb Ecol, 72, pp.741~758 (2016)
- Harfan DT, "Effectiveness of Pseudomonas sp. BOT4 in degrading cooking oil using nitrogen source of sodium nitrate and yeast extract", J Protobiont, 8, pp.106~114 (2019)
- Hinman A, "A stereoselective synthesis of(-)-tetrodotoxin", J Am Chem Soc, 125, pp.11510~11511 (2003)
- Jandhyala SM, "Role of the normal gut microbiota", World J Gastroenterol, 21, pp.8787~8803 (2015)
- Jiang W, "Multiscale structural evolution of citrate-triggered intrafibrillar and interfibrillar mineralization in dense collagen gels", J Struct Biol, 212, pp.107592~ (2020)
- Jumria A, "Identification and histopathology Flavobacterium columnare on tilapia(Oreochromis niloticus)", Samakia J Ilmu Perikan, 8, pp.17~22 (2017)
- Kaale LD, "Changes in water holding capacity and drip loss of Atlantic salmon(Salmo salar)muscle during superchilled storage", LWT-Food Sci Technol, 55, pp.528~535 (2014)
- Kanki M, "Histidine decarboxylases and their role in accumulation of histamine in tuna and dried saury", Appl Environ Microbiol, 73, pp.1467~1473 (2007)
- 김보라, "Deciphering Diversity Indices for a Better Understanding of Microbial Communities", Journal of Microbiology and Biotechnology, 27(12), pp.2089~2093 (2017), 한국미생물·생명공학회
- Lacapa R, "Study of decline in bottom fish quality of gill net catches at room temperature and cold storage", J Sain Sos Hum, 1, pp.14~25 (2021)
- Liu F, "Effects of astaxanthin and emodin on the growth, stress resistance and disease resistance of yellow catfish(Pelteobagrus fulvidraco)", Fish Shellfish Immunol, 51, pp.125~135 (2016)
- Lorentzen G, "A simulated e-commerce cold chain for fresh cod (Gadus morhua L.) products: applicability of selected TTIs and effects of pre-treatment and packaging", Food Packag Shelf Life, 31, pp.100794~ (2022)
- Martin M, "Cutadapt removes adapter sequences from high-throughput sequencing reads", EMBnet J, 17, pp.10~12 (2011)
- Meiriza Y, "The difference of characteristic milkfish(Chanos chanos Forsk)boneless in difference packaging during frozen storage", J Pengolah Bioteknol Has Perikan, 5, pp.36~43 (2016)
- Min D, "AXIOME3: automation, extension, and integration of microbial ecology", Gigascience, 10, pp.giab006~ (2021)
- Norita, "Kualitas ikan tongkol abu-abu(Thunnus tonggol)pada kondisi penyimpanan berbeda", J Pengolah Has Perikan Indones, 22, pp.490~497 (2019)
- Nurilmala M, "Chemical, microbiology changes and detection of hdc gene on longtail tuna thunnus tonggol during chilling temperature storage", J Ilmu Teknol Kelaut Tropis, 11, pp.285~296 (2019)
- Nurilmala M, "Detection of histamine-producing bacteria on tuna species using histidine decarboxylase(hdc)and 16S rRNA", Squalen Bull Mar Fish Postharvest Biotechnol, 15, pp.131~139 (2020)
- Pianusa AF, "Study of changes in the freshness quality of tuna(Euthynnus Affinis)soaked in seaweed extract(Eucheuma spinosum)and mangrove fruit extract(Sonneratia alba)", J Media Teknol Has Perikan, 3, pp.66~74 (2015)
- Quast C, "The SILVA ribosomal RNA gene database project : improved data processing and web-based tools", Nucleic Acids Res, 41, pp.D590~D596 (2013)
- "R: a language and environment for statistical computing. R Foundation for Statistical Computing" (2013)[URL]
- "RStudio: Integrated Development for R. RStudio" (2020)[URL]
- Rimoldi S, "Effect of a specific composition of short- and medium-chain fatty acid 1-monoglycerides on growth performances and gut microbiota of gilthead sea bream (Sparus aurata)", PeerJ, 6, pp.e5355~ (2018)
- Rimoldi S, "Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus labrax) fed low fishmeal and fish oil based diet", PLOS ONE, 15, pp.e0231494~ (2020)
- Robert FS, "Mathematics of planet earth: protecting our planet, learning from the past, safeguarding for the future", pp.1~22 (2019), Springer
- Rodríguez J, "Bacterial communities as indicators of environmental pollution by POPs in marine sediments", Environ Pollut, 268, pp.115690~ (2021)
- Sari RA, "The effect of gamma irradiation and freezing storage as an effort to improve food safety on “patin” fish (Pangasius hypoptalmus)", J Pangan Agroindustri, 5, pp.1~8 (2018)
- Schloss PD, "Amplicon sequence variants artificially split bacterial genomes into separate clusters", mSphere, 6, pp.e0019121~ (2021)
- Shehata HR, "Characterization of the microbiota of commercially traded finfish fillets", Food Res Int, 137, pp.109373~ (2020)
- Siburian ETP, "The effect of temperature and storage time on the growth of bacteria and fungi in milkfish", Unnes J Life Sci, 1, pp.101~105 (2012)
- Wang J, "Isolation of a Lysinibacillus fusiformis strain with tetrodotoxin-producing ability from puffer fish Fugu obscurus and the characterization of this strain", Toxicon, 56, pp.640~643 (2010)
- Willis AD, "Rarefaction, alpha diversity, and statistics", Front Microbiol, 10, pp.2407~ (2019)
- Wiranata K, "The development of cold chain system on fresh tuna fish(Euthynnus affinis)for fish retailer", J Biosist Tek Pertan, 6, pp.12~21 (2017)
- Zhang J, "Combined effects of polyethylene and organic contaminant on zebrafish(Danio rerio) : accumulation of 9-nitroanthracene, biomarkers and intestinal microbiota", Environ Pollut, 277, pp.116767~ (2021)
- Zhuang S, "Spoilage-related microbiota in fish and crustaceans during storage : research progress and future trends", Compr Rev Food Sci Food Saf, 20, pp.252~288 (2021)