The Expression of Carbonic Anhydrase (CA) IX/XII and Lymph Node Metastasis in Early Breast Cancer
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose The aim of study was to test by immunohistochemical (IHC) staining whether carbonic anhydrase (CA) 9 and 12 have an effect on sentinel lymph node (SLN) metastasis in early breast cancer and to find clinicopathologic factors associated with SLN metastasis. Materials and Methods Between June 2003 and June 2011, medical records of 470 patients diagnosed with breast cancer with pT1-2, pN0-2, and M0 were reviewed. Of these 470, 314 patients who under- went SLN biopsy±axillary dissection were subjects of this study. Using tissue microarray, IHC staining for CA9 and CA12 was performed. Clinicopathologic factors such as patient age, tumour size, lymphatic invasion, hormone receptor status, and the Ki-67 labeling index were analysed together. Results The mean age of all patients was 51.7 years. The mean number of harvested SLN was 3.62, and 212 patients (67.5%) had negative SLN. Lymphatic invasion, the Ki-67 labelling index of primary tumours, and CA9 staining of stromal cells, were independent risk factors for SLN metastasis in the multivariate analysis. In 33 patients (10.5%) without the three risk factors, no patient had SLN metastasis. In 80 patients without lymphatic invasion of primary tumours or CA9 staining of stromal cells, only four patients (5%) had positive SLN. Conclusion CA9 staining of stromal cells is an independent risk factor for SLN metastasis as well as lymphatic invasion and a low Ki-67 labelling index of primary tumours in patients with early breast cancer. IHC staining of primary tumours for CA12 was not associated with SLN metas- tasis.
Abstract
Purpose The aim of study was to test by immunohistochemical (IHC) staining whether carbonic anhydrase (CA) 9 and 12 have an effect on sentinel lymph node (SLN) metastasis in early breast cancer and to find clinicopathologic factors associated with SLN metastasis. Materials and Methods Between June 2003 and June 2011, medical records of 470 patients diagnosed with breast cancer with pT1-2, pN0-2, and M0 were reviewed. Of these 470, 314 patients who under- went SLN biopsy±axillary dissection were subjects of this study. Using tissue microarray, IHC staining for CA9 and CA12 was performed. Clinicopathologic factors such as patient age, tumour size, lymphatic invasion, hormone receptor status, and the Ki-67 labeling index were analysed together. Results The mean age of all patients was 51.7 years. The mean number of harvested SLN was 3.62, and 212 patients (67.5%) had negative SLN. Lymphatic invasion, the Ki-67 labelling index of primary tumours, and CA9 staining of stromal cells, were independent risk factors for SLN metastasis in the multivariate analysis. In 33 patients (10.5%) without the three risk factors, no patient had SLN metastasis. In 80 patients without lymphatic invasion of primary tumours or CA9 staining of stromal cells, only four patients (5%) had positive SLN. Conclusion CA9 staining of stromal cells is an independent risk factor for SLN metastasis as well as lymphatic invasion and a low Ki-67 labelling index of primary tumours in patients with early breast cancer. IHC staining of primary tumours for CA12 was not associated with SLN metas- tasis.
참고문헌 (25)
- Sugie T, "Comparison of the indocyanine green fluorescence and blue dye methods in detection of sentinel lymph nodes in early-stage breast cancer", Ann Surg Oncol, 20, pp.2213~2218 (2013)
- Veronesi U, "Twenty-year follow-up of a randomized study comparing breast-conserving surgery with radical mastectomy for early breast cancer", N Engl J Med, 347, pp.1227~1232 (2002)
- Ugras S, "Estrogen receptor, progesterone receptor, and HER2 status predict lymphovascular invasion and lymph node involvement", Ann Surg Oncol, 21, pp.3780~3786 (2014)
- 이종훈, "Predictors of Axillary Lymph Node Metastases (ALNM) in a Korean Population with T1-2 Breast Carcinoma: Triple Negative Breast Cancer has a High Incidence of ALNM Irrespective of the Tumor Size", Cancer Research and Treatment, 42(1), pp.30~36 (2010), 대한암학회
- Brahimi-Horn MC, "Hypoxia and cancer", J Mol Med(Berl), 85, pp.1301~1307 (2007)
- Lundgren K, "Hypoxia and breast cancer : prognostic and therapeutic implications", Cell Mol Life Sci, 64, pp.3233~3247 (2007)
- Milani M, "Targeting tumour hypoxia in breast cancer", Eur J Cancer, 44, pp.2766~2773 (2008)
- Shin HJ, "Carbonic anhydrase IX(CA9)modulates tumor-associated cell migration and invasion", J Cell Sci, 124(Pt 7), pp.1077~1087 (2011)
- Wykoff CC, "Expression of the hypoxia-inducible and tumorassociated carbonic anhydrases in ductal carcinoma in situ of the breast", Am J Pathol, 158, pp.1011~1019 (2001)
- McShane LM, "Reporting recommendations for tumor marker prognostic studies(remark)", Exp Oncol, 28, pp.99~105 (2006)
- Hammond ME, "American Society of Clinical Oncology", College of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer, 2010, pp.6, 195~197
- Wolff AC, "American Society of Clinical Oncology", Col-lege of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer, 2007, pp.25, 118~145
- Chia SK, "Prognostic significance of a novel hypoxia-regulated marker, carbonic anhydrase IX, in invasive breast carcinoma", J Clin Oncol, 19, pp.3660~3668 (2001)
- Aomatsu N, "Carbonic anhydrase 9 is associated with chemosensitivity and prognosis in breast cancer patients treated with taxane and anthracycline", BMC Cancer, 14, pp.400~ (2014)
- Schutze D, "Relevance of cellular and serum carbonic anhydrase IX in primary breast cancer", J Cancer Res Clin Oncol, 139, pp.747~754 (2013)
- Brockton NT, "High stromal carbonic anhydrase IX expression is associated with nodal metastasis and decreased survival in patients with surgically-treated oral cavity squamous cell carcinoma", Oral Oncol, 48, pp.615~622 (2012)
- Fiaschi T, "Carbonic anhydrase IX from cancer-associated fibroblasts drives epithelial-mesenchymal transition in prostate carcinoma cells", Cell Cycle, 12, pp.1791~1801 (2013)
- Hsieh MJ, "Carbonic anhydrase XII promotes invasion and migration ability of MDA-MB-231breast cancer cells through the p38 MAPK signaling pathway", Eur J Cell Biol, 89, pp.598~606 (2010)
- Barnett DH, "Estrogen receptor regulation of carbonic anhydrase XII through a distal enhancer in breast cancer", Cancer Res, 68, pp.3505~3515 (2008)
- Watson PH, "Carbonic anhydrase XII is a marker of good prognosis in invasive breast carcinoma", Br J Cancer, 88, pp.1065~1070 (2003)
- Brenin DR, "Factors correlating with lymph node metastases in patients with T1 breast cancer", Ann Surg Oncol, 8, pp.432~437 (2001)
- de Azambuja E, "Ki-67 as prognostic marker in early breast cancer : a meta-analysis of published studies involving 12, 155 patients", Br J Cancer, 96, pp.1504~1513 (2007)
- Bader AA, "T1 breast cancer : identification of patients at low risk of axillary lymph node metastases", Breast Cancer Res Treat, 76, pp.11~17 (2002)
- Koyama Y, "The association between sentinel lymph node metastasis and Ki-67 labeling index", Adv Breast Cancer Res, 2, pp.60~65 (2013)
- Terasaki-Fukuzawa Y, "Decreased nm23 expression, but not Ki-67labeling index, is significantly correlated with lymph node metastasis of breast invasive ductal carcinoma", Int J Mol Med, 9, pp.25~29 (2002)