Overexpression of α1 -protease Inhibitor and G alectin-1 in Radiation-induced Early Phase of Pulmonary Fibrosis
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Purpose: Radiation-induced pulmonary fibrosis (RIF) isa significant complication of radiotherapy for lung cancer. Despite the large number of studies, the molecularmechanisms of RIF are poorly understood. Therefore, thecomplex protein expression pattern in RIF was characterizedby identifying the proteins with an altered expressionlevel after thorax irradiation using two- dimensionalelectrophoresis (2-DE) and mass spectrometry. Materials and Methods: A mouse model of RIF wasused to examine the alteration of the lung proteomebecause of availability of murine data related to humancases and the abundance of murine fibrotic lung samples. A mouse model of RIF was induced in radiosensitiveC57BL/6 mice. Twenty-one weeks after 25 Gy irradiation,hematoxylin-eosin staining and hydroxyprolineassay confirmed the early-phase pulmonary fibrosis. Results: Lung samples from the irradiated and agematchedcontrol mice were used to generate 16 highquality 2-DE gels containing approximately 1,000 spots. Of the 31 significantly up- or down-regulated proteinspots, 17 were identified by MALDI-TOF/MS. Conclusions: Two important upregulated proteins werefound, the α1-protease inhibitor and galectin-1, whichmight be used as potential markers for the early phaseof RIF. (Cancer Res Treat. 2006;38:92-98)
Abstract
Purpose: Radiation-induced pulmonary fibrosis (RIF) isa significant complication of radiotherapy for lung cancer. Despite the large number of studies, the molecularmechanisms of RIF are poorly understood. Therefore, thecomplex protein expression pattern in RIF was characterizedby identifying the proteins with an altered expressionlevel after thorax irradiation using two- dimensionalelectrophoresis (2-DE) and mass spectrometry. Materials and Methods: A mouse model of RIF wasused to examine the alteration of the lung proteomebecause of availability of murine data related to humancases and the abundance of murine fibrotic lung samples. A mouse model of RIF was induced in radiosensitiveC57BL/6 mice. Twenty-one weeks after 25 Gy irradiation,hematoxylin-eosin staining and hydroxyprolineassay confirmed the early-phase pulmonary fibrosis. Results: Lung samples from the irradiated and agematchedcontrol mice were used to generate 16 highquality 2-DE gels containing approximately 1,000 spots. Of the 31 significantly up- or down-regulated proteinspots, 17 were identified by MALDI-TOF/MS. Conclusions: Two important upregulated proteins werefound, the α1-protease inhibitor and galectin-1, whichmight be used as potential markers for the early phaseof RIF. (Cancer Res Treat. 2006;38:92-98)
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