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REGULATION OF PULPAL MICROCIRCULATION BY CALCITONIN GENE-RELATED PEPTIDE

Regulation of pulpal microcirculation by calcitonin gene-related peptide

진명욱|김영경|김성교
대한치과보존학회 (Korean Academy Of Conservative Dentistry)|2005.11|Restorative Dentistry and Endodontics|제30권 제6호|pp. 470-476

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참고문헌

키워드

치수blood flow혈류신경성 염증neurogenic inflammationcalcitonin gene related peptide (CGRP)교감신경 자극laser Doppler flowmeter.dental pulpsympathetic nerve stimulation
주제분류치의학

초록

본 연구에서는 감각성 neuropeptide인 CGRP의 치수혈류 조절에 관해 교감신경과의 유기적 관계를 연구함으로써 CGRP의 치수혈류 조절기전을 밝히고자 하였다. 열두 마리의 전신마취된 고양이에서 실험하였으며 CGRP를 혈관을 통해 전신적 또는 국소적으로 투여하였다. 견치에서 치수혈류의 변화를 측정하고 paired t-test로 통계분석 하였으며 95% 수준에서 유의성을 검증하였다. CGRP(0.3 μg/kg)를 전신정맥으로 주사시, 전신혈압에 현저한 영향을 나타내면서 치수혈류는 평균 68.85%의 일차적인 증가와 감소를 보였고 이차적으로 다시 평균 161.8% 증가하였다가 감소하였다. CGRP를 저용량(0.03 μg/kg)으로 국소적으로 투여시, 치수혈류는 평균 2.92%의 미약한 증가를 나타내었다. 교감신경을 전기자극시 (10 Hz, 4 V, 1.5 ms), 전신혈압은 영향을 받지 않으면서 치수혈류가 유의하게 평균 57.88% 감소하였다. 교감신경 자극으로 치수혈류가 저하되어 있는 동안 주입한 CGRP는 저하된 치수혈류를 유의하게 회복시켰다. CGRP의 이 치수혈류 증가 효과는 CGRP8-37 에 의해 효과적으로 차단되었다.

Abstract

The purpose of this study was to investigate the function of calcitonin gene-related peptide (CGRP) in regulatory mechanism of pulpal microcirculation with the aim of elucidating neurogenic inflammation. Experiments were performed on twelve cats under general anesthesia. CGRP was administered through the femoral vein to see the systemic influence and through the external carotid artery to see the local effect. Sympathetic nerve to the dental pulp was stimulated electrically and pulpal blood flow (PBF) was measured with a laser Doppler flowmeter on the canine teeth to the drug administration. The paired variables of control and experimental data were compared by paired t-test and differences with p < 0.05 were considered statistically significant.Systemic administration of CGRP (0.3 μg/kg) exerted decreases in systemic blood pressure and caused changes in PBF with an initial increase followed by decrease and a more marked second increase and decrease. Close intra-arterial (i.a.) injection of CGRP (0.03 μg/kg) resulted in slight PBF increase. The effect of CGRP resulted in no significant increase in PBF in the presence of CGRP8-37. The electrical stimulation of the sympathetic nerve alone resulted in PBF decreases. The i.a. administration of CGRP following the electrical stimulation of the sympathetic nerve compensated the decreased PBF. Therefore, CGRP effectively blocked the sympathetic nerve stimulation-induced PBF decrease.Results of the present study have provided evidences that even though the local vasodilatory function of CGRP are weak, CGRP is effectively involved in blocking the vasoconstriction caused by sympathetic nerve stimulation in the feline dental pulp.

참고문헌 (23)

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관련 논문AI추천

Effects of endodontic treatment on salivary levels of CGRP and substance P: a pilot studyArslan Hakan, Köseoğlu Serhat, Yıldız Ezgi Doğanay대한치과보존학회 · Restorative Dentistry and Endodontics2020 · 제45권 제3호Interleukin-8 and MCP(Monocyte Chemoattractant Protein)-1 expression by the Human Dental Pulps in cultures stimulated with Substance P박상혁, 신한주, 최기운대한치과보존학회 · Restorative Dentistry and Endodontics2005 · 제30권 제3호Involvement of TRPA1 in the cinnamaldehyde-induced pulpal blood flow change in the feline dental pulp김도경, 김성교, 이문환대한치과보존학회 · Restorative Dentistry and Endodontics2016 · 제41권 제3호기계적으로 자극 받은 치아에서 국소마취가 치수혈류에 미치는 영향김성교, 박성호, 안동국, 추완식대한치과보존학회 · Restorative Dentistry and Endodontics2006 · 제31권 제4호흰쥐 치수에서 Er:YAG laser에 의한 노출손상에 따른 조직학적 변화 및 CGRP 단백질의 발현김규탁, 김상봉, 김흥중, 박종태, 양재호, 이난영, 이상호대한소아치과학회 · 대한소아치과학회지2008 · 제35권 제1호Local application of NK1 receptor antagonists and pulpal blood flow in cat김성교, 김영경, 안동국, 유현미, 이호정, 추완식대한치과보존학회 · Restorative Dentistry and Endodontics2004 · 제29권 제3호

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