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Prevotella intermedia와 Prevotella nigrescens의 세균내독소에 대한 연구 -화학적 분석 및 면역생물학적 활성 평가-

Chemical and Immunobiological Characterization of Lipopolysaccharides from Prevotella intermedia and Prevotella nigrescens

김성조
대한치주과학회 (The Korean Academy Of Periodontology)|2004.06|Journal of Periodontal & Implant Science|제34권 제2호|pp. 461-474

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참고문헌

주제분류치의학

초록

Abstract

The purpose of this study was to assess some biological activities of lipopolysaccharides (LPSs) from P. intermedia and P. nigrescens. LPS was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. TNF-α production was determined by enzyme-linked immunosorbent assay. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. LPS from P. intermedia demonstrated higher KDO content than those from two stains of P. nigrescens. LPSs from P. intermedia and P. nigrescens were mitogenic for spleen cells of BALB/C mouse. The present study clearly shows that LPSs from P. intermedia and P. nigrescens fully induced iNOS expression and NO production in RAW264.7 cells in the absence of other stimuli. Moreover, LPSs from P. intermedia and P. nigrescens clearly induced TNF-α production in RAW264.7 cells. The biological activities of LPS from P. intermedia was found to be comparable to those of P. nigrescens LPS. The ability of LPSs from P. intermedia and P. nigrescens to promote the production of NO and TNF-α may be important in the pathogenesis of inflammatory periodontal disease.

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관련 논문AI추천

Prevotella nigrescens의 추출물, 지질다당질 및 세균 배양액에 대한 독성의 특성김신무, 김은숙, 조지현대한임상검사과학회 · 대한임상검사과학회지2003 · 제35권 제1호Porphyromonas gingivalis, Prevotella intermedia, 그리고 Prevotella nigrescens에서의 hemin 결합 단백질에 대한 연구김성조대한치주과학회 · Journal of Periodontal & Implant Science2006 · 제36권 제1호Porphyromonas gingivalis의 각종 표면성분이 싸이토카인 형성에 미치는 영향김성조대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제3호Prevotella nigrescens에서의 Hemin조절 세포막 단백질의 순수분리 및 특성분석김경미, 김성조, 최점일대한치주과학회 · Journal of Periodontal & Implant Science2002 · 제32권 제2호Prevotella nigrescens가 배양된 세포에 미치는 영향김은숙, 유용욱, 이수종, 임미경, 한경수, 한진순대한치과보존학회 · Restorative Dentistry and Endodontics2002 · 제27권 제2호Comparison of inflammatory cytokine-inducing activity of lipopolysaccharides from major periodontal bacteria강인철, 김소희대한구강생물학회 · International Journal of Oral Biology2019 · 제44권 제4호

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