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Porphyromonas gingivalis의 각종 표면성분이 싸이토카인 형성에 미치는 영향

The cytokine-inducing activities of surface components of the periodontopathogenic bacterium Porphyromonas gingivalis

김성조
대한치주과학회 (The Korean Academy Of Periodontology)|2005.09|Journal of Periodontal & Implant Science|제35권 제3호|pp. 799-811

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참고문헌

주제분류치의학

초록

Abstract

This study was carried out to examine the potency of the three surface compo- nents from Porphyromonas gingivalis to stimulate the murine macrophage cell line RAW264.7 to synthesize the pro-inflammatory cytokine tumor necrosis factor alpha(TNF-α) and nitric oxide (NO). Lipopolysaccharide(LPS), lipid A-associated proteins(LAP) and saline-extractable surface -associated material(SAM) were isolated from P. gingivalis 381. TNF-α release into culture supernatants was determined by two-site ELISA. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. Western blot analysis of iNOS and analysis of reverse transcription(RT)-PCR products were carried out. The surface components extracted from this bacterium were almost equally potent in stimulating release of TNF-α and NO by RAW264.7 cells. TNF-α that was being measured immunologically was due to activation of TNF-α gene transcription. The present study clearly shows that P. gingivalis surface components fully induced iNOS expression in RAW264.7 cells in the absence of other stimuli. The ability of P. gingivalis surface components to promote the production of TNF-α and NO may be important in the pathogenesis of inflammatory periodontal disease.

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관련 논문AI추천

Porphyromonas gingivalis바이오필름에 의한 숙주 면역반응의 교란김성조, 전우석, 최점일대한치주과학회 · Journal of Periodontal & Implant Science2002 · 제32권 제4호조골세포에서 Aggregatibacter actinomycetemcomitans 생균의 파골세포분화유도 cytokine 발현 유도능 및 침투능김경대, 김민영, 유윤정, 이양신, 차정헌, 최호길대한치주과학회 · Journal of Periodontal & Implant Science2007 · 제37권 제3호Porphyromonas gingivalis, Prevotella intermedia, 그리고 Prevotella nigrescens에서의 hemin 결합 단백질에 대한 연구김성조대한치주과학회 · Journal of Periodontal & Implant Science2006 · 제36권 제1호사람 치은 섬유아세포에서의 Tannerella forsythia 전세균, 막단백질, 당지질에 의한 염증성 사이토카인 발현구영, 구기태, 김정은, 김태일, 류인철, 이성훈, 이용무, 정종평, 최봉규대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제3호Porphyromonas gingivalis의 열충격단백 발현조절 환경인자에 관한 연구최점일대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제1호Prevotella intermedia와 Prevotella nigrescens의 세균내독소에 대한 연구 -화학적 분석 및 면역생물학적 활성 평가-김성조대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제2호

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