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속단의 dichloromethane 분획물이 마우스 두개골 세포의 분화에 미치는 영향

The Effects of Dichloromethane fraction of Phlomodis Radix(DFPR) on differentiation of Mouse Calvarial Cell

유윤정|김윤철|유형근|최성호|김종관|윤정호|정의원|김동진
대한치주과학회 (The Korean Academy Of Periodontology)|2004.12|Journal of Periodontal & Implant Science|제34권 제4호|pp. 791-805

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참고문헌

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초록

Abstract

The purpose of this study was to evaluate the effects of DFPR on differentiation of mouse calvarial cell in vitro, to examine the possibility for periodontal regeneration. 10μg/ml of DFPR was used as experimental concentration. osteogenic medium only was assigned as control, Experimental 1 was supplemented with 10nM dexamethasone, Experimental 2 with 10μg/ml DFPR and Experimental 3 with 10nM dexamethasone + 10μg/ml DFPR. cellular activity was evaluated by MTT method at 8, 12, 16 days, expression of mRNA of ALP, osteopontin, osteocalcin, collagen type-1 was detected by RT-PCR method at 4, 8, 12, 16 days of culture . extent of mineralization was observed by Von Kossa staining at 16 day of culture. The results are as follows 1)Any acceleration of differentiation was not observed at expression of differentiation marker, 2) Decrease in expression of extracelluar matrix and in bone nodule formation was observed The results suggested that DFPR have negative effect on the rate of differentiation on rat calvarial cell, decrease extracelluar matrix formation ,decrease bone nodule formation. Ongoing studies are necessary in order to determine effect of DFPR on periodontal regeneration.

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