유기산 완충용액의 불소농도가 상아질의 재광화에 미치는 영향
THE EFFECTS OF THE FLUORIDE CONCENTRATION OF ACIDULATED BUFFER SOLUTIONS ON DENTINE REMINERALIZATION
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
상아질의 재광화에 적당한 불소농도에 대하여는 서로 다른 주장이 존재하므로 불소가 병소 내 무기질의 분포와 수산화인회석 결정성장에 미치는 영향을 분석하기 위하여 이 연구를 시행하였다. 치아절편을 유산완충 탈회용액에 넣어 인공 치아우식을 형성한 후, 재광화 효과를 관찰하기 위해 불소농도가 각각 1, 2, 4 ppm인 유산완충 재광화용액에서 7일간 유지시켰다. 우식 진행과 재광화 양상을 관찰하기 위하여 탈회 2일, 재광화 7일 등 총 9일간 편광현미경으로 관찰하였으며 수산화인회석결정의 변화를 관찰하기 위하여서는 정상상아질, 탈회 2일군, 재광화 7일군의 파절시편을 주사전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 모든 군에서 병소상부의 무기질침착과 병소하부의 무기질소실이 동시에 일어났다. 2. 불소농도가 증가하면 우식 병소의 무기질침착이 증가하였다. 3. 수산화인회석결정이 커졌다. 본 실험의 결과에 의하면, 상아질의 우식과 재광화 과정이 단순히 탈회 또는 재광화만이 독립적으로 일어나는 과정이 아니고 이 두 과정이 동시에 일어나는 동력학적인 과정이다. 또한 불소농도의 증가와 함께 재광화 양상도 증가하였고, 이러한 재광화 는 유기기질망 주위의 수산화인회석결정을 중심으로 진행되었다.
Abstract
The aim of this vitro-study is to evaluate the effects of fluoride on remineralization of artificial dentine caries. 10 sound permanent premolars, which were extracted for orthodontic reason within 1 week, were used for this study. Artificial dentine caries was created by using a partially saturated buffer solution for 2days with grounded thin specimens and fractured whole-body specimens. Remineralization solutions with three different fluoride concentration (1 ppm, 2 ppm and 4 ppm) were used on demineralized-specimens for 7 days. Polarizing microscope and scanning electron microscope were used for the evaluation of the mineral distribution profile and morphology of crystallites of hydroxyapatite. The results were as follows : 1. When treated with the fluoride solutions, the demineralized dentine specimens showed remineralization of the upper part and demineralization of the lower part of the lesion body simultaneously. 2. As the concentration of fluoride increased, the mineral precipitation in the caries dentine increased. The mineral precipitation mainly occurred in the surface layer in 1 and 2 ppm- specimens and in the whole lesion body in 4 ppm -specimens. 3. When treated with the fluoride solution, the hydroxyapatite crystals grew. This crystal growth was even observed in the lower part of the lesion body which had shown the loss of mineral.
참고문헌 (29)
- Featherstone JD, "A mechanism for dental caries based on chemical processes and diffusion phenomena during in-vitro caries simulation on human tooth enamel", Arch Oral Biol, 24, pp.101~112 (1979)
- Margolis HC, "Physicochemical perspectives on the cariostatic mechanisms of systemic and topical fluorides", J Dent Res, 69, pp.606~613 (1990)
- Dean HT, "Domestic water and dental caries. V. Additional studies of the relation of fluoride in domestic waters to dental caries experience in 4,425 white children aged 12 to 14 years, of 13 cities in 4 states", Publ.Hlth.Rep, 57, pp.1155~1179 (1942)
- Cate JM, "Comparison of the in vivo effect of a 0 and 1,500 ppmF MFP toothpaste on fluoride uptake, acid resistance and lesion remineralization", Caries Res, 20, pp.193~201 (1986)
- Arends J, "Remineralization of bovine dentine in vitro. The influence of the F content in solution on mineral distribution", Caries Res, 23, pp.309~314 (1989)
- Silverstone LM, "The effect of saliva and calcifying solutions upon the histological appearance of enamel caries", Caries Res, 2, pp.87~93 (1968)
- Varughese K, "Crystal growth of calcium apatites in dilute solutions containing fluoride", Calcif Tissue Int, 33, pp.431~439 (1981)
- 한원섭, "인공치아우식의 재광화에 미치는 불소의 영향", 대한치과보존학회지, 21, pp.161~173 (1996)
- Kawasaki K, "Relationship between mineral distributions in dentine lesions and subsequent remineralization in vitro", Caries Res, 34, pp.395~403 (2000)
- Levine RS, "Further studies on the remineralization of human carious dentine in vitro", Arch Oral Biol, 18, pp.1351~1356 (1973)
- Groeneveld A, "The mineral content of decalcified surface enamel. A combined microprobe- quantitative microradiography study", Caries Res, 8, pp.267~274 (1974)
- Aoba T, "X-ray diffraction study on remineralization using synthetic hydroxyapatite pellets", Caries Res, 12, pp.223~230 (1978)
- Darling AI, "Some observations on amelogenesis imperfecta and calcification of the dental enamel", Proc R Soc Med, 49, pp.759~765 (1956)
- Featherstone JD, "Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles", Caries Res, 17, pp.385~391 (1983)
- Haikel Y, "Scanning electron microscopy of the human enamel surface layer of incipient carious lesions", Caries Res, 17, pp.1~13 (1983)
- Vizioli MR, "Dichroism and birefringence in human carious dentine", Ann Histochim, 15, pp.131~140 (1970)
- Dietz W, "Influence of cementum on the demineralization and remineralization processes of root surface caries in vitro", Acta Odontol Scand, 60, pp.241~247 (2002)
- Arends J, "Dentine caries in vivo. Combined scanning electron microscopic and microradiographic investigation", Caries Res, 23, pp.36~41 (1989)
- Takuma S, "Demineralization and Remineralization of tooth substance- an ultrastructural basis for caries prevention", J Dent Res, pp.2146~2156 (1980)
- LeGeros RZ, "Formation and Stability of Synthetic Apatites. Calcium Phosphates in Oral Biology and Medicine", pp.87~107 (1991), Karger
- Holmen L, "A scanning electron microscopic study of surface changes during development of artificial caries", Caries Res, 19, pp.11~21 (1985)
- Hayashi Y, "High resolution electron microscopy of the interface between dental calculus and denture resin", Scanning Microsc, 9, pp.149~425 (1995)
- 박정원, "유기산 완충용액의 포화도가 법랑질 및 상아질의 재광화에 미치는 영향과 수산화인회석의 AFM 관찰", 대한치과보존학회지, 25, pp.459~473 (2000)
- Takuma S, "Some structural aspects of carious lesions in human dentine", Bull. Tokyo dent. Coll., 10, pp.173~181 (1969)
- LeGeros RZ, "Chemical and crystallographic events in the caries process", J Dent Res, 69, pp.567~574 (1990)
- Watson ML, "The development of the hamster lower incisor as observed by electron microscopy", Am J Anat, 95, pp.109~ (1954)
- Levine RS, "Remineralization of human carious dentine in vitro", Arch Oral Biol, 17, pp.1005~1008 (1972)
- Klont B, "Remineralization of bovine incisor root lesions in vitro: the role of the collagenous matrix", Caries Res, 25, pp.39~45 (1991)
- Scott DB, "Structural aspects of dental caries", J Dent Res, 53, pp.165~178 (1974)