불소와 칼슘의 법랑질 재광화 효과에 대한 생체외 연구
Effect of fluoride and calcium on enamel remineralization in vitro
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초록
침식된 치아 법랑질의 효과적인 재광화 방법에 관한 연구의 일환으로, 0.1~1.0% 구연산 용액에서 60분간 탈회시킨 사람 소구치 법랑질을 인공타액, 100ppm 및 1000ppm 불소가 첨가된 인공타액, 1000ppm 칼슘이 첨가된 인공타액, 100ppm 불소와 1000ppm 칼슘이 첨가된 인공타액에 각각 넣고 재광화시키면서, 3시간과 6시간 경과시에 표면미세경도를 측정하였다. 불소나 칼슘을 첨가하지 않은 인공타액 및 불소 100ppm을 첨가한 인공타액에서는 6시간 동안 유의한 재광화가 일어나지 않았으며, 불소 1000ppm을 첨가한 인공타액에서는 3시간 후에 유의한 재광화가 관찰되었고 3시간부터 6시간 사이에는 유의한 재광화가 일어나지 않았으며, 칼슘 1000ppm을 첨가한 인공타액 및 불소 100ppm과 칼슘 1000ppm을 첨가한 인공타액에서는 3시간 후와 6시간 후에 유의한 재광화가 관찰되었다(유의수준 P=0.05). 불소나 칼슘을 첨가하지 않은 인공타액 또는 불소 100ppm을 첨가한 인공타액에 비해 불소 100ppm과 칼슘 1000ppm을 첨가한 인공타액의 재광화효과가 유의하게 더 컸으며, 불소를 100ppm 또는 1000ppm으로 첨가한 것, 불소 농도를 100ppm에서 1000ppm으로 증가시킨 것, 칼슘을 1000ppm으로 첨가한 것은 재광화효과를 유의하게 증가시키지 않았다.
Abstract
The purpose of study was to observe the effect of fluoride and calcium on enamel remineralizaton in vitro. Human premolar enamel specimens were prepared by demineralization in 0.1~1.0% citric acid for 60 minutes. They were remineralized for 6 hours in one of the following solutions : (1) artificial saliva, (2) artificial saliva with 100ppmF, (3) artificial saliva with 1000ppmF, (4) artificial saliva with 1000ppmCa, and (5) artificial saliva with 100ppmF and 1000ppmCa. No significant remineralization was occurred in artificial saliva and artificial saliva with 100ppmF. Significant remineralization was observed in artificial saliva with 1000ppmF at 3 hours, and in artificial saliva with 1000ppmCa and artificial saliva with 100ppmF and 1000ppmCa at 3 and 6 hours(P<0.05). The remineralization effect of artificial saliva with 100ppmF and 1000ppmCa was greater than that of artificial saliva or artificial saliva with 100ppmF. Addition of F to 100ppm or 1000ppm, addition of Ca to 1000ppm, and increasing the concentration of F from 100ppm to 1000ppm did not significantly increase the remineralization.
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