형광제자리부합법을 이용한 치은열구세포 내의 치주염 유발 세균의 관찰
Visualization of periodontopathic bacteria within crevicular epithelial cells with fluorescence in situ hybridization
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Purpose: Periodontal pathogens can invade the host tissue. Morphologic studies have revealed bacteria within the pocket epithelium, gingival connective tissues, alveolar bone, and oral epithelium. The objective of this study was to visualize and evaluate presence of Porphyromonas gingivalis and Tannerella forsythia in crevicular epithelial cells of periodontally healthy subjects and chronic periodontitis patients. Materials and Methods: A total of 666 crevicular epithelial cells in the samples obtained from 27 chronic periodontitis patients and 9 healthy volunteers were examined. Specific probes for P. gingivalis and T. forsythia and a universal probe for detection of all eubacteria targeting 16S rRNA for fluorescence in situ hybridization was used in conjunction with confocal laser scanning microscopy. Results: 98.99% of sulcular epithelial cells from healthy volunteers and 84.40% of pocket epithelial cells from periodontitis patients were found to harbor bacteria. P. gingivalis and T. forsythia were discovered more often in crevicular epithelial cells from periodontitis patients. Conclusion: P. gingivalis and T. forsythia can invade crevicular epithelial cells and intracellular bacteria may act as a source of bacteria for persistent infection. (J Korean Acad Periodontol 2008;38:691-698)
Abstract
Purpose: Periodontal pathogens can invade the host tissue. Morphologic studies have revealed bacteria within the pocket epithelium, gingival connective tissues, alveolar bone, and oral epithelium. The objective of this study was to visualize and evaluate presence of Porphyromonas gingivalis and Tannerella forsythia in crevicular epithelial cells of periodontally healthy subjects and chronic periodontitis patients. Materials and Methods: A total of 666 crevicular epithelial cells in the samples obtained from 27 chronic periodontitis patients and 9 healthy volunteers were examined. Specific probes for P. gingivalis and T. forsythia and a universal probe for detection of all eubacteria targeting 16S rRNA for fluorescence in situ hybridization was used in conjunction with confocal laser scanning microscopy. Results: 98.99% of sulcular epithelial cells from healthy volunteers and 84.40% of pocket epithelial cells from periodontitis patients were found to harbor bacteria. P. gingivalis and T. forsythia were discovered more often in crevicular epithelial cells from periodontitis patients. Conclusion: P. gingivalis and T. forsythia can invade crevicular epithelial cells and intracellular bacteria may act as a source of bacteria for persistent infection. (J Korean Acad Periodontol 2008;38:691-698)
참고문헌 (25)
- Noiri Y, "An immunohistochemical study on the localization of Porphyromonas gingivalis, Campylobacter rectus and Actinomyces viscosus in human periodontal pockets", J Periodontal Res, 32, pp.598~607 (1997)
- Rudney JD, "Intracellular Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in buccal epithelial cells collected from human subjects", Infection and Immunity, 69, pp.2700~2707 (2001)
- Fillery ED, "Identification of microorganisms in immunopathological mechanism on human gingivitis", J Dent Res, 61, pp.253~ (1982)
- Saglie FR, "Intragingival occurrence of Actinobacillus actinomycemtemcomitans and Bacteroides gingivalis in active destructive periodontal lesions", J Periodontol, 59, pp.259~ (1988)
- Carranza FA, Jr, "Scanning and transmission electron microscopic study of tissue-invading microorganisms in localized juvenile periodontitis", J Periodontol, 54, pp.598~ (1983)
- Sreenivasan PK, "Requirements for invasion of epithelial cells by Actinobacillus actimomycetemcomitans", Infect Immun, 61, pp.1239~ (1993)
- Han YW, "Interactions between periodontal bacteria and human oral epithelial cells: Fusobacterium nucleatum adheres to and invades epithelial cells", Infect Immun, 68, pp.3140~ (2000)
- Sandros J, "Porphyromonas gingivalis invades oral epithelial cells in vitro", J Periodontal Res, 28, pp.219~ (1993)
- Wang B, "Interaction of Treponema denticola with HEp-2 cells", J Dent Res, 72, pp.324~ (1993)
- Madianos PN, "Porphyromonas gingivalis FDC381 multiplies and persistis within the human oral epithelial cells in vitro", Infect Immun, 64, pp.660~664 (1996)
- Dale BA, "Periodontal epithelium: a newly recognized role in health and disease", Periodontol, 30, pp.70~78 (2002)
- Moter A, "Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms", J Microb Methods, 41, pp.85~112 (2000)
- Sakamoto M, "Molecular analysis of human oral microbiota", J Periodontal Res, 40, pp.277~285 (2005)
- Claridge JE 3rd, "Impact of 16S rRNA sequence analysis for identification of bacteria on clinical microbiology and infectious diseases", Clin Microbiol Rev, 17, pp.840~862 (2004)
- Földes-Papp Z, "Laser scanning confocal fluorescence microscopy: an overview", International Immunopharmacology, 3, pp.1715~1729 (2003)
- Michalet X, "The power and prospects of fluorescence microscopies and spectroscopies", Annu Rev Biophys Biomol Struct, 32, pp.161~182 (2003)
- Dibart S, "Identification of bacterial species on or in crevicular epithelial cells from healthy and periodontally diseased patients using DNA-DNA hybridization", Oral Microbiol Immunol, 13, pp.30~35 (1998)
- Colombo AV, "Identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions", J Med Microbiol, 55, pp.606~615 (2006)
- Colombo AV, "Identification of intracellular oral species within human crevicular epithelial cells from subjects with chronic periodontitis by fluorescence in situ hybridization", J Periodontal Res, 42, pp.236~243 (2007)
- Loy A, "probeBase - an online resource for rRNA-targeted oligonucleotide probes: new features 2007", Nucleic Acids Res, 35, pp.D800~D804 (2007)
- Lamont RJ, "In or out: the invasiveness of oral bacteria", Periodontol, 30, pp.61~69 (2002)
- Sandros J, "Cytokine responses of oral epithelial cells to Porphyromonas gingivalis infection", J Dent Res, 79, pp.1804~1814 (2000)
- Andrian E, "Porphrymonas gingivalis-epithelial cell interactions in periodontitis", 85, pp.392~403 (2006)
- Rudney JD, "Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis are components of a polymicrobial intracellular flora within human buccal cells", J Dent Res, 84, pp.59~63 (2005)
- Johnson JD, "Persistence of extracrevicular bacterial reservoirs after treatment of aggressive periodontitis", J Periodontol, 79, pp.2305~2312 (2008)
