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논문KCI 등재

형광제자리부합법을 이용한 치은열구세포 내의 치주염 유발 세균의 관찰

Visualization of periodontopathic bacteria within crevicular epithelial cells with fluorescence in situ hybridization

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참고문헌

주제분류치의학

초록

Purpose: Periodontal pathogens can invade the host tissue. Morphologic studies have revealed bacteria within the pocket epithelium, gingival connective tissues, alveolar bone, and oral epithelium. The objective of this study was to visualize and evaluate presence of Porphyromonas gingivalis and Tannerella forsythia in crevicular epithelial cells of periodontally healthy subjects and chronic periodontitis patients. Materials and Methods: A total of 666 crevicular epithelial cells in the samples obtained from 27 chronic periodontitis patients and 9 healthy volunteers were examined. Specific probes for P. gingivalis and T. forsythia and a universal probe for detection of all eubacteria targeting 16S rRNA for fluorescence in situ hybridization was used in conjunction with confocal laser scanning microscopy. Results: 98.99% of sulcular epithelial cells from healthy volunteers and 84.40% of pocket epithelial cells from periodontitis patients were found to harbor bacteria. P. gingivalis and T. forsythia were discovered more often in crevicular epithelial cells from periodontitis patients. Conclusion: P. gingivalis and T. forsythia can invade crevicular epithelial cells and intracellular bacteria may act as a source of bacteria for persistent infection. (J Korean Acad Periodontol 2008;38:691-698)

Abstract

Purpose: Periodontal pathogens can invade the host tissue. Morphologic studies have revealed bacteria within the pocket epithelium, gingival connective tissues, alveolar bone, and oral epithelium. The objective of this study was to visualize and evaluate presence of Porphyromonas gingivalis and Tannerella forsythia in crevicular epithelial cells of periodontally healthy subjects and chronic periodontitis patients. Materials and Methods: A total of 666 crevicular epithelial cells in the samples obtained from 27 chronic periodontitis patients and 9 healthy volunteers were examined. Specific probes for P. gingivalis and T. forsythia and a universal probe for detection of all eubacteria targeting 16S rRNA for fluorescence in situ hybridization was used in conjunction with confocal laser scanning microscopy. Results: 98.99% of sulcular epithelial cells from healthy volunteers and 84.40% of pocket epithelial cells from periodontitis patients were found to harbor bacteria. P. gingivalis and T. forsythia were discovered more often in crevicular epithelial cells from periodontitis patients. Conclusion: P. gingivalis and T. forsythia can invade crevicular epithelial cells and intracellular bacteria may act as a source of bacteria for persistent infection. (J Korean Acad Periodontol 2008;38:691-698)

참고문헌 (25)

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관련 논문AI추천

DNA 형광 염색을 이용한 치은열구상피부착 세균에 관한 연구계승범, 신승윤, 양승민, 이상현대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제4호한국인 만성 치주염 환자에서 치주질환 원인균의 동정김두일, 박정은, 윤정호, 이대실, 이승일, 조규성, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제2호사람 치은 섬유아세포에서의 Tannerella forsythia 전세균, 막단백질, 당지질에 의한 염증성 사이토카인 발현구영, 구기태, 김정은, 김태일, 류인철, 이성훈, 이용무, 정종평, 최봉규대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제3호Quantitative Detection of Periodontopathogens in Gingival Crevicular Fluid Using Real-time PCR한경순, Seo Young Kim대한통합치과학회 · 대한통합치과학회지2025 · 제14권 제1호한국 성인형 치주염 환자의 치주낭 내 세균분포에 관한 암시야 현미경적 연구남기윤, 박정민, 엄흥식, 이인경, 장범석대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제2호치주질환이 없는 청년의 치은연상 및 치은연하 치면세균막에 존재하는 치주질환 관련 4종 세균의 분포 비교국중기, 김병옥, 김수관, 김지연, 김화숙, 유소영, 장현선대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제2호

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